Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,749,448	T→C	C73R (TGC→CGC)	sgbU →	putative L‑xylulose 5‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,749,448	0	T	C	100.0%	207.5 / NA	57	C73R (TGC→CGC)	sgbU	putative L‑xylulose 5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/29);  total (28/29)

GCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATG  >  NC_000913/3749307‑3749583                                                                                                                                              |                                                                                                                                        gCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCT‑‑‑‑CGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATgcgc                                                                                                                                       >  2:136979/1‑140 (MQ=255)   aaaaaGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCT‑‑‑‑CGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCt                                                                                                                                     <  1:136979/140‑1 (MQ=255)      aaGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGcc                                                                                                                                      >  2:287387/1‑140 (MQ=255)         cTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGt                                                                                                                                   <  1:585452/140‑1 (MQ=255)           gCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTcc                                                                                                                                 >  2:1294566/1‑140 (MQ=255)                  tGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACAtc                                                                                                                          >  1:584362/1‑140 (MQ=255)                       ttGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGc                                                                                                                     <  2:546661/140‑1 (MQ=255)                        tGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCt                                                                                                                    <  1:755225/140‑1 (MQ=255)                         gTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCtt                                                                                                                   >  2:852172/1‑140 (MQ=255)                          tCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCttt                                                                                                                  <  2:35127/140‑1 (MQ=255)                                  tCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTgg                                                                                                          <  1:852172/140‑1 (MQ=255)                                   cGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGt                                                                                                         <  1:29542/140‑1 (MQ=255)                                    ggTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTa                                                                                                        >  2:735909/1‑140 (MQ=255)                                      tGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGc                                                                                                      <  1:735909/140‑1 (MQ=255)                                      tGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGc                                                                                                      <  1:959666/140‑1 (MQ=255)                                      tGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGc                                                                                                      <  1:419943/140‑1 (MQ=255)                                      tGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGc                                                                                                      <  1:365499/140‑1 (MQ=255)                                             aaCCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACg                                                                                               >  2:434526/1‑140 (MQ=255)                                                cgacgaACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAgg                                                                                            <  2:127997/140‑1 (MQ=255)                                                cgacgaACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCATCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAgg                                                                                            <  1:795831/140‑1 (MQ=255)                                                 gacgaACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGc                                                                                           <  1:434526/140‑1 (MQ=255)                                                  acgaACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCa                                                                                          >  2:487368/1‑140 (MQ=255)                                                  acgaACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCa                                                                                          >  1:559233/1‑140 (MQ=255)                                                  acgaACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCa                                                                                          >  2:24478/1‑140 (MQ=255)                                                          ctctCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGa                                                                                  >  1:1306487/1‑140 (MQ=255)                                                                 gTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGgcg                                                                           <  2:243611/140‑1 (MQ=255)                                                                  tCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGgcgc                                                                          >  1:45971/1‑140 (MQ=255)                                                                     tGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCggg                                                                       <  1:287387/140‑1 (MQ=255)                                                                       aCTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGaa                                                                     >  2:170878/1‑140 (MQ=255)                                                                         tGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAAt                                                                   >  2:398463/1‑140 (MQ=255)                                                                           gAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCa                                                                 >  2:1395801/1‑140 (MQ=255)                                                                           gAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCa                                                                 <  2:500409/140‑1 (MQ=255)                                                                                       aaGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGa                                                     <  2:20108/140‑1 (MQ=255)                                                                                                gCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTgg                                            >  2:368784/1‑140 (MQ=255)                                                                                                 cTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGgc                                           >  2:195186/1‑140 (MQ=255)                                                                                                 cTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGgc                                           >  2:1025670/1‑140 (MQ=255)                                                                                                    gTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGgcgcg                                        <  2:584362/140‑1 (MQ=255)                                                                                                         cgcgATGATCGAAACAGGTGTTGGGATCCCGTAGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGAtc                                   >  2:349262/1‑140 (MQ=255)                                                                                                           cgATGATCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGAtctc                                 <  1:1395801/140‑1 (MQ=255)                                                                                                              tgatCGAAACAGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGc                              >  2:187366/1‑140 (MQ=255)                                                                                                                        aGGTGTTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCa                    >  2:497931/1‑140 (MQ=255)                                                                                                                          gtgtTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATc                  >  2:633023/1‑140 (MQ=255)                                                                                                                          gtgtTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATc                  >  1:892550/1‑140 (MQ=255)                                                                                                                            gtTGGGATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCa                >  2:1103732/1‑140 (MQ=255)                                                                                                                               gggATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCt             >  2:1332976/1‑140 (MQ=255)                                                                                                                                ggATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTg            <  2:650762/140‑1 (MQ=255)                                                                                                                                 gATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTgg           <  2:1306487/140‑1 (MQ=255)                                                                                                                                 gATCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTgg           <  1:195186/140‑1 (MQ=255)                                                                                                                                  aTCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGc          <  1:1332976/140‑1 (MQ=255)                                                                                                                                  aTCCCGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGc          <  1:654141/140‑1 (MQ=255)                                                                                                                                    cccGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAg        >  2:980735/1‑140 (MQ=255)                                                                                                                                     ccGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGGGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCt             <  2:1304083/134‑1 (MQ=255)                                                                                                                                     ccGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGGGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCt             >  1:1304083/1‑134 (MQ=255)                                                                                                                                     ccGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAgg       >  1:821947/1‑140 (MQ=255)                                                                                                                                      cGTCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGt      >  2:397528/1‑140 (MQ=255)                                                                                                                                        tCGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTa    >  1:1181814/1‑140 (MQ=255)                                                                                                                                         cGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTAt   <  2:1181814/140‑1 (MQ=255)                                                                                                                                         cGATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTAt   <  2:892550/140‑1 (MQ=255)                                                                                                                                          gATGCGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATg  <  1:349262/140‑1 (MQ=255)                                                                                                                                              |                                                                                                                                        GCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAGGACTTCGCTGGTTGCCGCGATGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAGGCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCGATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATG  >  NC_000913/3749307‑3749583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑