Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,918,437	T→C	Y474C (TAC→TGC)	atpA ←	ATP synthase F1 complex subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,918,437	0	T	C	100.0%	263.1 / NA	72	Y474C (TAC→TGC)	atpA	ATP synthase F1 complex subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/27);  total (45/27)

AAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCGGCGCATACTGTTTCTGTTTCAG  >  NC_000913/3918303‑3918567                                                                                                                                       |                                                                                                                                   aaGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCATCGGAGCGTGATCACGGTCGACGCAAGcc                                                                                                                               <  1:1317619/140‑1 (MQ=255)  aGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCca                                                                                                                              >  2:201199/1‑140 (MQ=255)  aGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCca                                                                                                                              >  2:244053/1‑140 (MQ=255)  aGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCca                                                                                                                              >  1:718088/1‑140 (MQ=255)  aGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCca                                                                                                                              >  1:394539/1‑140 (MQ=255)  aGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCca                                                                                                                              >  1:360082/1‑140 (MQ=255)        cAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCagag                                                                                                                        <  2:373844/140‑1 (MQ=255)               tACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTc                                                                                                                 >  2:768926/1‑140 (MQ=255)                   aGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAg                                                                                                             <  1:548023/140‑1 (MQ=255)                       ttGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGc                                                                                                         <  2:654136/140‑1 (MQ=255)                          ggTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCagag                                                                                                                        >  1:705261/1‑122 (MQ=255)                          ggTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCagag                                                                                                                        <  2:705261/122‑1 (MQ=255)                               cTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTc                                                                                                 >  1:456973/1‑140 (MQ=255)                               cTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAACTGCCAATTTTc                                                                                                 >  2:925312/1‑140 (MQ=255)                                tttGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCg                                                                                                <  2:357970/140‑1 (MQ=255)                                 ttGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGa                                                                                               <  1:201199/140‑1 (MQ=255)                                    aaGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAg                                                                                            >  1:487765/1‑140 (MQ=255)                                      ggAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGtt                                                                                          >  2:1195426/1‑140 (MQ=255)                                           cGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACa                                                                                     <  2:535943/140‑1 (MQ=255)                                                aTGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGc                                                                                <  1:925312/140‑1 (MQ=255)                                                  gCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCa                                                                              <  2:814580/140‑1 (MQ=255)                                                  gCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCa                                                                              >  2:264318/1‑140 (MQ=255)                                                    cTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAgg                                                                            <  1:1291494/140‑1 (MQ=255)                                                    cTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAgg                                                                            >  1:781808/1‑140 (MQ=255)                                                     tttCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGt                                                                           >  2:295653/1‑140 (MQ=255)                                                       tCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTaa                                                                         >  1:611101/1‑140 (MQ=255)                                                       tCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTaa                                                                         <  1:1195426/140‑1 (MQ=255)                                                        cAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAAc                                                                        >  2:151403/1‑140 (MQ=255)                                                          gCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCa                                                                      >  2:28334/1‑140 (MQ=255)                                                             tGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGt                                                                   >  2:1107905/1‑140 (MQ=255)                                                              gCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGtt                                                                  >  2:1155222/1‑140 (MQ=255)                                                              gCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGtt                                                                  <  1:757020/140‑1 (MQ=255)                                                                cTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTct                                                                >  1:341725/1‑140 (MQ=255)                                                                   cGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTctgct                                                             <  1:347455/140‑1 (MQ=255)                                                                        tcgtcgTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTTGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGaa                                                        <  2:476072/140‑1 (MQ=255)                                                                        tcgtcgTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGaa                                                        <  2:1101235/140‑1 (MQ=255)                                                                        tcgtcgTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGaa                                                        <  1:911284/140‑1 (MQ=255)                                                                        tcgtcgTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCACCAGGTAACCACGTTCTGCTGCGaa                                                        <  1:244053/140‑1 (MQ=255)                                                                         cgtcgtTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAAc                                                       >  2:1168907/1‑140 (MQ=255)                                                                                        cGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGActgc                                        >  2:344524/1‑140 (MQ=255)                                                                                        cGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGActgc                                        >  2:1132494/1‑140 (MQ=255)                                                                                        cGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGActgc                                        >  2:1203159/1‑140 (MQ=255)                                                                                        cGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGActgc                                        >  1:1140895/1‑140 (MQ=255)                                                                                               gTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCaa                                 >  2:448135/1‑140 (MQ=255)                                                                                               gTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCaa                                 >  2:1280145/1‑140 (MQ=255)                                                                                                 tGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACg                               <  1:295653/140‑1 (MQ=255)                                                                                                     ctctTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACa                           <  2:1140895/140‑1 (MQ=255)                                                                                                     ctctTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACa                           >  2:1170087/1‑140 (MQ=255)                                                                                                     ctctTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACa                           >  1:1079210/1‑140 (MQ=255)                                                                                                     ctctTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACa                           >  1:942626/1‑140 (MQ=255)                                                                                                     ctctTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACa                           <  1:448135/140‑1 (MQ=255)                                                                                                       ctTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATc                         <  1:1170087/140‑1 (MQ=255)                                                                                                       ctTGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATc                         <  1:554971/140‑1 (MQ=255)                                                                                                         tGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCgg                       >  2:1054719/1‑140 (MQ=255)                                                                                                         tGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCgg                       >  1:301112/1‑140 (MQ=255)                                                                                                         tGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCgg                       <  2:394539/140‑1 (MQ=255)                                                                                                         tGCATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCgg                       >  2:67337/1‑140 (MQ=255)                                                                                                           cATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCGgcg                     <  2:360082/140‑1 (MQ=255)                                                                                                           cATCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCGgcg                     <  2:611101/140‑1 (MQ=255)                                                                                                             tCAACGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCGGCGCa                   >  2:805508/1‑140 (MQ=255)                                                                                                               aaCGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCGGCGCATa                 <  2:456973/140‑1 (MQ=255)                                                                                                                aCGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCGGCGCATAc                >  2:944470/1‑140 (MQ=255)                                                                                                                aCGGAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCGGCGCATAc                >  2:378164/1‑140 (MQ=255)                                                                                                                  ggAGCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCGGCGCATActg              >  1:948453/1‑140 (MQ=255)                                                                                                                    aGCGTGATCACGGTCGACGCAAGCCGGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCGGCGCATActgtt            >  2:473958/1‑140 (MQ=255)                                                                                                                     gCGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCGGCGCATActgttt           >  1:1384376/1‑140 (MQ=255)                                                                                                                      cGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCGGCGCATActgtt            >  2:493317/1‑138 (MQ=255)                                                                                                                      cGTGATCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCGGCGCATActgtt            <  1:493317/138‑1 (MQ=255)                                                                                                                          aTCACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCGGCGCATActgtttctgtt      >  1:839154/1‑140 (MQ=255)                                                                                                                            cACGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCGGCGCATACtgtttctgtttc    >  1:660413/1‑140 (MQ=255)                                                                                                                             aCGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCCGCGCAACGGACATCGGCGCATACTGTTTCTGTTTca   >  2:1333609/1‑140 (MQ=255)                                                                                                                              cGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCGGCGCATACTGTTTCTGTTTcag  >  2:809475/1‑140 (MQ=255)                                                                                                                              cGGTCGACGCAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCGGCGCATACTGTTTCTGTTTcag  >  2:1157498/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                   AAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAGCGGCTTCGAAGCTGCCAATTTTCGACAGTTCAACATCCGCCAGGTAACCACGTTCTGCTGCGAACAGAACCAGAGACTGCTGCGCAACGGACATCGGCGCATACTGTTTCTGTTTCAG  >  NC_000913/3918303‑3918567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑