Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,953,304	G→A	G274D (GGC→GAC)	ilvE →	branched‑chain‑amino‑acid aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,953,304	0	G	A	100.0%	318.8 / NA	90	G274D (GGC→GAC)	ilvE	branched‑chain‑amino‑acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/46);  total (44/46)

GCGAAAGAGCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCG  >  NC_000913/3953171‑3953440                                                                                                                                      |                                                                                                                                          gCGAAAGAGCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAgg                                                                                                                                     <  2:782380/140‑1 (MQ=255) gCGAAAGAGCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAgg                                                                                                                                     <  1:1188215/140‑1 (MQ=255) gCGAAAGAGCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAgg                                                                                                                                     <  2:390082/140‑1 (MQ=255)  cGAAAGAGCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGc                                                                                                                                    <  2:310329/140‑1 (MQ=255)       gagCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTtg                                                                                                                               >  2:549545/1‑140 (MQ=255)        agCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTtgt                                                                                                                              <  2:546667/140‑1 (MQ=255)          ctaggAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTgg                                                                                                                            <  1:756780/137‑1 (MQ=255)            gggAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCATGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGcc                                                                                                                          <  1:895936/140‑1 (MQ=255)            gggAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGcc                                                                                                                          <  1:853174/140‑1 (MQ=255)              gAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAAT‑ACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCgg                                                                                                                       >  2:778994/1‑140 (MQ=255)                   gAAGCACGCGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGACCGGTACGGCGGCAGAAATCACGCCAGTGCGCAACGAAGACGGGATTCAGGTTGACGAAGGCCGTTGTGGCCCGGCTaa                                                                                                                   >  2:479405/1‑139 (MQ=255)                       tACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCaaa                                                                                                               <  2:909598/140‑1 (MQ=255)                           tGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCAGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCaa                                                                                                                >  1:1044816/1‑135 (MQ=255)                           tGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCAGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCaa                                                                                                                <  2:1044816/135‑1 (MQ=255)                            gAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCAt                                                                                                          >  2:1343290/1‑140 (MQ=255)                            gAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCAt                                                                                                          <  1:208040/140‑1 (MQ=255)                             aGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCAtt                                                                                                         <  1:7896/140‑1 (MQ=255)                                 ggTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGc                                                                                                     >  1:616159/1‑140 (MQ=255)                                  gTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAg                                                                                                      <  1:973211/138‑1 (MQ=255)                                  gTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAg                                                                                                      >  2:973211/1‑138 (MQ=255)                                     cTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGc                                                                                                 >  2:751484/1‑140 (MQ=255)                                     cTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTGTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACAAAGGCCGGTGTGGCCCGGTTACCAAACGCATTCATCAAGc                                                                                                 >  2:1236115/1‑140 (MQ=255)                                       gTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCct                                                                                               >  1:1153663/1‑140 (MQ=255)                                        tCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCctt                                                                                              >  1:899196/1‑140 (MQ=255)                                        tCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCctt                                                                                              >  2:209686/1‑140 (MQ=255)                                          gcgcgAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCcttct                                                                                            <  2:452712/140‑1 (MQ=255)                                          gcgcgAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCcttct                                                                                            <  1:238206/140‑1 (MQ=255)                                            gcgAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCttcttc                                                                                          >  1:95043/1‑140 (MQ=255)                                            gcgAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCttcttc                                                                                          <  2:1153663/140‑1 (MQ=255)                                            gcgAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGGTACCAAACGCATTCAACCAGCCttcttc                                                                                          >  2:855008/1‑140 (MQ=255)                                             cgAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCg                                                                                         <  1:874563/140‑1 (MQ=255)                                             cgAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCg                                                                                         <  1:549545/140‑1 (MQ=255)                                               aaTCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGc                                                                                       <  2:159944/140‑1 (MQ=255)                                               aaTCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGc                                                                                       <  1:751484/140‑1 (MQ=255)                                                  cccTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCctc                                                                                    >  2:1237330/1‑140 (MQ=255)                                                          cTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGc                                                                                       >  1:550677/1‑129 (MQ=255)                                                          cTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGc                                                                                       <  2:550677/129‑1 (MQ=255)                                                           tGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGc                                                                           >  1:427663/1‑140 (MQ=255)                                                            ggCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCg                                                                          >  1:875851/1‑140 (MQ=255)                                                            ggCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCg                                                                          >  1:630652/1‑140 (MQ=255)                                                            ggCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCg                                                                          >  1:626135/1‑140 (MQ=255)                                                            ggCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCg                                                                          >  1:1142680/1‑140 (MQ=255)                                                            ggCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCg                                                                          >  1:309422/1‑140 (MQ=255)                                                            ggCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCg                                                                          >  2:181272/1‑140 (MQ=255)                                                              cGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGaa                                                                        >  1:508450/1‑140 (MQ=255)                                                              cGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGaa                                                                        >  1:353874/1‑140 (MQ=255)                                                               ggATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGaaa                                                                       <  1:1236115/140‑1 (MQ=255)                                                                 aTGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTTTTCGGCCTCTTCACTGGCGAAAcc                                                                     >  2:1334643/1‑140 (MQ=255)                                                                 aTGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAAcc                                                                     >  1:856923/1‑140 (MQ=255)                                                                 aTGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAAcc                                                                     >  2:16731/1‑140 (MQ=255)                                                                 aTGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAAcc                                                                     <  2:899196/140‑1 (MQ=255)                                                                  tGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCg                                                                    <  1:1192442/140‑1 (MQ=255)                                                                   gAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGGTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAAACGa                                                                   >  2:1392560/1‑140 (MQ=255)                                                                          ttATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑aaa                                                           <  1:1215122/140‑1 (MQ=255)                                                                          ttATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑aaa                                                           <  1:479405/140‑1 (MQ=255)                                                                          ttATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑aaa                                                           <  1:16731/140‑1 (MQ=255)                                                                              gTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATggg                                                       >  2:317579/1‑140 (MQ=255)                                                                               tCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATgggg                                                      <  2:427663/140‑1 (MQ=255)                                                                               tCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATgggg                                                      <  1:390261/140‑1 (MQ=255)                                                                                 cGGTACGGCGGCAGAAATCGCGCCAGTGCGCAGCGGAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGGTACCAAACCCAATCCAGAATGCTTCCTCGGCCTCTTCACAGGCGAAACCGAAGATAAAATGGGGc                                                     >  2:1396559/1‑140 (MQ=255)                                                                                   gTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATgggg                                                      >  1:709012/1‑136 (MQ=255)                                                                                   gTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATgggg                                                      <  2:709012/136‑1 (MQ=255)                                                                                   gTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTgg                                                  >  2:1390575/1‑140 (MQ=255)                                                                                    tACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATgggg                                                      <  2:1349262/135‑1 (MQ=255)                                                                                    tACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATgggg                                                      >  1:1349262/1‑135 (MQ=255)                                                                                    tACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGt                                                 <  1:1334643/140‑1 (MQ=255)                                                                                    tACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGt                                                 <  1:317579/140‑1 (MQ=255)                                                                                        gcggcAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCGTTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGa                                             <  1:1027743/140‑1 (MQ=255)                                                                                        gcggcAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGa                                             >  2:516467/1‑140 (MQ=255)                                                                                            cAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATgggg                                                      >  1:1058777/1‑127 (MQ=255)                                                                                            cAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATgggg                                                      <  2:1058777/127‑1 (MQ=255)                                                                                            cAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCaa                                         >  1:1103403/1‑140 (MQ=255)                                                                                              gAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTTACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGt                                       >  1:1352396/1‑140 (MQ=255)                                                                                                       ccAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCaata                              <  1:209686/140‑1 (MQ=255)                                                                                                       ccAGTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCaata                              <  2:749929/140‑1 (MQ=255)                                                                                                          gTGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCaataaat                           >  2:325006/1‑140 (MQ=255)                                                                                                           tGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCaataaata                          >  1:233014/1‑140 (MQ=255)                                                                                                           tGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCaataaata                          >  1:1163682/1‑140 (MQ=255)                                                                                                           tGCGCAGCGTAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCaataaata                          >  1:849043/1‑140 (MQ=255)                                                                                                                    tAGACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATg                 >  1:934264/1‑140 (MQ=255)                                                                                                                     aGACGGTATTCAGGTTGACGAAGGCCGTTGTGACCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATgg                >  2:1246946/1‑140 (MQ=255)                                                                                                                      gACGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATggg               <  1:1390575/140‑1 (MQ=255)                                                                                                                       aCGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGa              <  2:616159/140‑1 (MQ=255)                                                                                                                       aCGGTATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGa              <  1:778994/140‑1 (MQ=255)                                                                                                                           tATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGgc          <  2:309422/140‑1 (MQ=255)                                                                                                                           tATTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGgc          <  2:630652/140‑1 (MQ=255)                                                                                                                            aTTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGgca         <  2:1352396/140‑1 (MQ=255)                                                                                                                            aTTCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGgca         >  1:191691/1‑140 (MQ=255)                                                                                                                              tCAGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGgcacg       >  2:952721/1‑140 (MQ=255)                                                                                                                                aGGTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGgcacgca     <  1:1343290/140‑1 (MQ=255)                                                                                                                                 ggTTGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGgcacgcac    >  1:183193/1‑140 (MQ=255)                                                                                                                                   ttGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATggg               <  1:780425/127‑1 (MQ=255)                                                                                                                                   ttGACGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCg  <  1:1299262/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                          GCGAAAGAGCTGGGAATTGAAGTACGTGAGCAGGTGCTGTCGCGCGAATCCCTGTACCTGGCGGATGAAGTGTTTATGTCCGGTACGGCGGCAGAAATCACGCCAGTGCGCAGCGTAGACGGTATTCAGGTTGGCGAAGGCCGTTGTGGCCCGGTTACCAAACGCATTCAGCAAGCCTTCTTCGGCCTCTTCACTGGCGAAACCGAAGAT‑AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCG  >  NC_000913/3953171‑3953440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑