Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,968,246	A→G	M111V (ATG→GTG)	rfe →	UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,968,246	0	A	G	100.0%	266.9 / NA	73	M111V (ATG→GTG)	rfe	UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/34);  total (39/34)

TACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTT  >  NC_000913/3968117‑3968380                                                                                                                                  |                                                                                                                                       tACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGtt                                                                                                                              >  1:1035443/1‑140 (MQ=255)    tataTTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCgg                                                                                                                           <  1:1022525/140‑1 (MQ=255)      tatTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGgtg                                                                                                                                >  1:660451/1‑133 (MQ=255)      tatTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGgtg                                                                                                                                <  2:660451/133‑1 (MQ=255)      tatTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCa                                                                                                                         <  1:789066/140‑1 (MQ=255)      tatTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCa                                                                                                                         <  2:216674/140‑1 (MQ=255)          ccGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCt                                                                                                                     >  1:1330519/1‑140 (MQ=255)           cGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCtt                                                                                                                    >  1:1051263/1‑140 (MQ=255)           cGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCtt                                                                                                                    >  1:134083/1‑140 (MQ=255)             catgcatCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTa                                                                                                                  >  2:1036389/1‑140 (MQ=255)             catgcatCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTa                                                                                                                  <  1:1036389/140‑1 (MQ=255)              atgcatCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTAt                                                                                                                 >  1:1030228/1‑140 (MQ=255)              atgcatCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTAt                                                                                                                 >  1:1080727/1‑140 (MQ=255)              atgcatCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTAt                                                                                                                 >  1:283386/1‑140 (MQ=255)              atgcatCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTAt                                                                                                                 >  1:46351/1‑140 (MQ=255)                  atCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCa                                                                                                             <  1:960587/140‑1 (MQ=255)                  atCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCa                                                                                                             >  2:712177/1‑140 (MQ=255)                  atCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCa                                                                                                             <  2:1199565/140‑1 (MQ=255)                  atCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCa                                                                                                             <  2:902681/140‑1 (MQ=255)                  atCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCa                                                                                                             >  2:28282/1‑140 (MQ=255)                     tctctATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTa                                                                                                          >  1:1262611/1‑140 (MQ=255)                       tctATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGc                                                                                                        <  1:1291086/140‑1 (MQ=255)                       tctATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGc                                                                                                        <  2:169155/140‑1 (MQ=255)                        ctATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGcc                                                                                                       <  2:134083/140‑1 (MQ=255)                        ctATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGcc                                                                                                       <  2:1330519/140‑1 (MQ=255)                        ctATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGcc                                                                                                       <  1:17236/140‑1 (MQ=255)                        ctATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGcc                                                                                                       <  2:1117514/140‑1 (MQ=255)                            ctcGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCa                                                                                                             <  1:446842/130‑1 (MQ=255)                            ctcGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCa                                                                                                             >  2:446842/1‑130 (MQ=255)                              cGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCAACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGtt                                                                                                 >  2:67312/1‑140 (MQ=255)                               gCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTta                                                                                                >  1:842990/1‑140 (MQ=255)                                cTTGTGCTGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTtat                                                                                               >  2:1255593/1‑140 (MQ=255)                                cTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGcc                                                                                                       <  1:291733/132‑1 (MQ=255)                                cTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGcc                                                                                                       >  2:291733/1‑132 (MQ=255)                                cTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGcc                                                                                                       >  1:543482/1‑132 (MQ=255)                                cTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGcc                                                                                                       <  2:543482/132‑1 (MQ=255)                                cTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTtat                                                                                               >  1:97432/1‑140 (MQ=255)                                   gtgCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATc                                                                                            >  2:596686/1‑140 (MQ=255)                                     gCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAGATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCtt                                                                                          >  1:984316/1‑140 (MQ=255)                                     gCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCtt                                                                                          >  2:1016266/1‑140 (MQ=255)                                     gCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCtt                                                                                          >  2:601231/1‑140 (MQ=255)                                     gCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCtt                                                                                          <  1:28282/140‑1 (MQ=255)                                       cGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAGATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTg                                                                                        <  2:984316/140‑1 (MQ=255)                                       cGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTg                                                                                        <  1:1016266/140‑1 (MQ=255)                                       cGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTg                                                                                        <  1:1233225/140‑1 (MQ=255)                                        ggTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTgg                                                                                       <  2:384214/140‑1 (MQ=255)                                         gtgtGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGc                                                                                      <  2:46351/140‑1 (MQ=255)                                           gtgCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTc                                                                                    <  1:712177/140‑1 (MQ=255)                                           gtgCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGACGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTc                                                                                    <  2:176564/140‑1 (MQ=255)                                             gCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCt                                                                                  >  1:194313/1‑140 (MQ=255)                                                    ttCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGAt                                                                           <  2:194313/140‑1 (MQ=255)                                                    ttCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGAt                                                                           >  1:1176062/1‑140 (MQ=255)                                                      cATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATgg                                                                         >  1:1170871/1‑140 (MQ=255)                                                            cgcgcTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCg                                                                   <  2:1080727/140‑1 (MQ=255)                                                              cgcTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATGTCTTTGGCTCCTGGGAGATGGTGCTCGGa                                                                 <  2:109088/140‑1 (MQ=255)                                                                                       cGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTAt                                        >  1:189249/1‑140 (MQ=255)                                                                                       cGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTAt                                        >  1:585454/1‑140 (MQ=255)                                                                                       cGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTAt                                        >  1:251635/1‑140 (MQ=255)                                                                                           aaaaTCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGc                                    >  1:400411/1‑140 (MQ=255)                                                                                                 cGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGAc                                             >  2:537898/1‑125 (MQ=255)                                                                                                 cGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGAc                                             <  1:537898/125‑1 (MQ=255)                                                                                                 cGTGCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTACCGTCTg                              >  1:898358/1‑140 (MQ=255)                                                                                                    gCCACCATACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGggc                           <  2:1176062/140‑1 (MQ=255)                                                                                                      caccaTACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGggcgg                         <  2:251635/140‑1 (MQ=255)                                                                                                      caccaTACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGggcgg                         <  1:601231/140‑1 (MQ=255)                                                                                                        ccaTACAGGCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGcc                       >  2:352331/1‑140 (MQ=255)                                                                                                                gCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCa                      >  2:719630/1‑133 (MQ=255)                                                                                                                gCCGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCa                      <  1:719630/133‑1 (MQ=255)                                                                                                                 ccGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCg              <  2:842990/140‑1 (MQ=255)                                                                                                                  cGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGt             >  2:608825/1‑140 (MQ=255)                                                                                                                  cGCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGt             >  2:21910/1‑140 (MQ=255)                                                                                                                   gCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGtt            >  2:494724/1‑140 (MQ=255)                                                                                                                   gCTGTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGtt            >  2:25437/1‑140 (MQ=255)                                                                                                                      gTTGGCATTGTTGTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCaa         <  2:1035443/140‑1 (MQ=255)                                                                                                                             ttgttgTGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGtt  <  1:25437/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       TACTATATTCCGCATGCATCTCTCTATCTCGCTTGTGCCGGTGTGCTTGTTTTCATTGGCGCGCTGGATGACCGTTTTGATATCAGCGTAAAAATCCGTGCCACCATACAGGCCGCTGTTGGCATTGTTATGATGGTGTTCGGCAAGCTTTATCTCAGTAGCCTGGGTTATATCTTTGGCTCCTGGGAGATGGTGCTCGGACCGTTTGGTTACTTCCTGACGCTATTTGCCGTCTGGGCGGCCATTAATGCGTTCAACATGGTT  >  NC_000913/3968117‑3968380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑