Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,974,520	T→C	G18G (GGT→GGC)	rffC →	dTDP‑4‑amino‑4,6‑dideoxy‑D‑galactose acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,974,520	0	T	C	100.0%	248.2 / NA	68	G18G (GGT→GGC)	rffC	dTDP‑4‑amino‑4,6‑dideoxy‑D‑galactose acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/29);  total (39/29)

CTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGA  >  NC_000913/3974384‑3974655                                                                                                                                         |                                                                                                                                        ctggctCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGtt                                                                                                                                      <  2:568904/140‑1 (MQ=255)        gATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAgcg                                                                                                                               <  2:166285/140‑1 (MQ=255)          tGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAgcg                                                                                                                               >  2:567580/1‑138 (MQ=255)          tGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAgcg                                                                                                                               <  1:567580/138‑1 (MQ=255)                gggTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTg                                                                                                                       <  1:659146/140‑1 (MQ=255)                gggTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTg                                                                                                                       <  1:583834/140‑1 (MQ=255)                     tgAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCAt                                                                                                                  <  1:734437/140‑1 (MQ=255)                        aGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTAc                                                                                                               <  2:150819/140‑1 (MQ=255)                        aGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTAc                                                                                                               >  1:670333/1‑140 (MQ=255)                            tgctgcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCt                                                                                                           >  1:139252/1‑140 (MQ=255)                            tgctgcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCt                                                                                                           >  1:731918/1‑140 (MQ=255)                            tgctgcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCt                                                                                                           >  2:795485/1‑140 (MQ=255)                            tgctgcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCt                                                                                                           >  1:454733/1‑140 (MQ=255)                            tgctgcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCt                                                                                                           >  2:829132/1‑140 (MQ=255)                            tgctgcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCt                                                                                                           >  2:1118492/1‑140 (MQ=255)                            tgctgcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCt                                                                                                           >  2:338069/1‑140 (MQ=255)                            tgctgcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCt                                                                                                           >  1:708031/1‑140 (MQ=255)                            tgctgcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCt                                                                                                           >  2:288390/1‑140 (MQ=255)                             gctgcCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTg                                                                                                          >  1:681308/1‑140 (MQ=255)                                     ttCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCg                                                                                                  <  2:835611/140‑1 (MQ=255)                                     ttCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCg                                                                                                  <  2:464664/140‑1 (MQ=255)                                              gAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACg                                                                                         <  2:454733/140‑1 (MQ=255)                                               aaaaCTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGc                                                                                        >  2:84736/1‑140 (MQ=255)                                               aaaaCTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGc                                                                                        >  2:142229/1‑140 (MQ=255)                                                      gCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGAcgc                                                                                 >  1:72654/1‑140 (MQ=255)                                                         aCGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGtt                                                                              <  1:569825/140‑1 (MQ=255)                                                          cGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTa                                                                             <  2:708031/140‑1 (MQ=255)                                                            gCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAgc                                                                           <  2:621849/140‑1 (MQ=255)                                                               aaTATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGcc                                                                        <  2:180435/140‑1 (MQ=255)                                                               aaTATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAAACGCGTTAGCGcc                                                                        <  2:1256713/140‑1 (MQ=255)                                                                  atCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTg                                                                     <  1:1043669/140‑1 (MQ=255)                                                                      gctgGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCa                                                                 >  2:1323328/1‑140 (MQ=255)                                                                           aGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCAc                                                                <  2:462181/136‑1 (MQ=255)                                                                           aGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCAc                                                                >  1:462181/1‑136 (MQ=255)                                                                           aGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGt                                                            >  2:794831/1‑140 (MQ=255)                                                                           aGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGt                                                            >  2:361706/1‑140 (MQ=255)                                                                           aGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGt                                                            >  1:1282533/1‑140 (MQ=255)                                                                            gTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTg                                                           <  1:76230/140‑1 (MQ=255)                                                                            gTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTg                                                           <  1:84736/140‑1 (MQ=255)                                                                             ttACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGc                                                          >  2:104261/1‑140 (MQ=255)                                                                               aCTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAg                                                        <  2:1165703/140‑1 (MQ=255)                                                                               aCTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAg                                                        >  2:179976/1‑140 (MQ=255)                                                                                 ttCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGc                                                      >  1:1198716/1‑140 (MQ=255)                                                                                    gTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCaa                                                   >  1:59218/1‑140 (MQ=255)                                                                                     tGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCaaa                                                  >  2:689484/1‑140 (MQ=255)                                                                                     tGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCaaa                                                  >  1:286641/1‑140 (MQ=255)                                                                                     tGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCaaa                                                  >  1:1214920/1‑140 (MQ=255)                                                                                      gCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCaaaa                                                 >  1:1199882/1‑140 (MQ=255)                                                                                          gTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGc                                             <  1:361706/140‑1 (MQ=255)                                                                                           tCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCg                                            <  1:931115/140‑1 (MQ=255)                                                                                           tCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCg                                            <  1:288390/140‑1 (MQ=255)                                                                                               cgcCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCAt                                        >  1:1019594/1‑140 (MQ=255)                                                                                                   aGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCaaa                                    <  2:532336/140‑1 (MQ=255)                                                                                                        tGAGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACgg                               <  2:853212/140‑1 (MQ=255)                                                                                                          aGCCACTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGCGCAGGCCAAAATTGCGGCATCAAATACgggg                             >  1:604308/1‑139 (MQ=255)                                                                                                               cTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACt                        >  2:1092176/1‑140 (MQ=255)                                                                                                               cTAACCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACt                        >  1:1058253/1‑140 (MQ=255)                                                                                                                 aaCCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTgg                      <  2:670333/140‑1 (MQ=255)                                                                                                                  aCCTGGGAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGa                     <  1:794831/140‑1 (MQ=255)                                                                                                                      gggAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGcc                 >  2:1219812/1‑140 (MQ=255)                                                                                                                       ggAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGccc                >  1:1020334/1‑140 (MQ=255)                                                                                                                       ggAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGccc                >  2:1118501/1‑140 (MQ=255)                                                                                                                       ggAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACACCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTg                            >  2:483490/1‑128 (MQ=255)                                                                                                                       ggAAAACGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACACCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTg                            <  1:483490/128‑1 (MQ=255)                                                                                                                            aCGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCaa           >  2:94104/1‑140 (MQ=255)                                                                                                                             cGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAAc          >  2:141999/1‑140 (MQ=255)                                                                                                                             cGCCTTCTTTGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAAc          >  1:758289/1‑140 (MQ=255)                                                                                                                                     ttGGCGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGa  <  1:795485/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCAATATCTGCTGGAGTTACTTCGTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGA  >  NC_000913/3974384‑3974655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑