Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,023,933	A→G	H127R (CAC→CGC)	tatD →	3' ‑> 5' ssDNA/RNA exonuclease TatD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,023,933	0	A	G	100.0%	220.4 / NA	60	H127R (CAC→CGC)	tatD	3' ‑> 5' ssDNA/RNA exonuclease TatD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/38);  total (22/38)

GCGCAGCCAGAAGTGGTGGCGATTGGTGAATGTGGTCTCGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCACTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATAT  >  NC_000913/4023797‑4024065                                                                                                                                         |                                                                                                                                     gcgcAGCCAGAAGTGGTGGCGATTGGTGAATGTGGTCTCGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTg                                                                                                                                   <  1:411972/140‑1 (MQ=255)    cAGCCAGAAGTGGTGGCGATTGGTGAATGTGGTCTCGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTcg                                                                                                                                <  1:115816/140‑1 (MQ=255)      gCCAGAAGTGGTGGCGATTGGTGAATGTGGTCTCGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTcgcg                                                                                                                              <  2:1004406/140‑1 (MQ=255)                 tggCGATTGGTGAATGTGGTCTCGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAg                                                                                                                   <  2:217168/140‑1 (MQ=255)                 tggCGATTGGTGAATGTGGTCTCGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAg                                                                                                                   <  1:722900/140‑1 (MQ=255)                 tggCGATTGGTGAATGTGGTCTCGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAg                                                                                                                   <  2:1218771/140‑1 (MQ=255)                 tggCGATTGGTGAATGTGGTCTCGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAg                                                                                                                   >  1:682410/1‑140 (MQ=255)                 tggCGATTGGTGAATGTGGTCTCGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAg                                                                                                                   <  2:202036/140‑1 (MQ=255)                 tggCGATTGGTGAATGTGGTCTCGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAg                                                                                                                   <  1:130086/140‑1 (MQ=255)                 tggCGATTGGTGAATGTGGTCTCGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAg                                                                                                                   <  1:1285465/140‑1 (MQ=255)                 tggCGATTGGTGAATGTGGTCTCGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAg                                                                                                                   <  1:918296/140‑1 (MQ=255)                            gAATGTGGTCTCGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGAc                                                                                                        <  2:231431/140‑1 (MQ=255)                                  ggTCTCGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCt                                                                                                  >  2:199122/1‑140 (MQ=255)                                  ggTCTCGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCt                                                                                                  >  1:40788/1‑140 (MQ=255)                                   gTCTCGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTg                                                                                                 >  2:429690/1‑140 (MQ=255)                                   gTCTCGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTg                                                                                                 >  2:372764/1‑140 (MQ=255)                                    tctcGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTgg                                                                                                >  1:1219121/1‑140 (MQ=255)                                    tctcGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTgg                                                                                                >  2:1301198/1‑140 (MQ=255)                                        gACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGcc                                                                                            <  2:1125052/140‑1 (MQ=255)                                        gACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGcc                                                                                            >  1:560126/1‑140 (MQ=255)                                         aCTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCg                                                                                           <  1:799914/140‑1 (MQ=255)                                         aCTTTAACCGCAACTTCTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCg                                                                                           >  1:1171144/1‑140 (MQ=255)                                           tttAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGGAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTg                                                                                         <  2:612655/140‑1 (MQ=255)                                           tttAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTg                                                                                         <  2:1061702/140‑1 (MQ=255)                                           tttAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCACCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTg                                                                                         <  2:1219121/140‑1 (MQ=255)                                             tAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACAt                                                                                                      <  2:1002907/125‑1 (MQ=255)                                             tAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACAt                                                                                                      >  1:1002907/1‑125 (MQ=255)                                                ccGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTgg                                                                                    <  2:935834/140‑1 (MQ=255)                                                  gCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGAt                                                                                  <  2:13895/140‑1 (MQ=255)                                                     aCTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTg                                                                                         <  2:1201876/130‑1 (MQ=255)                                                     aCTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTg                                                                                         >  1:1201876/1‑130 (MQ=255)                                                     aCTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATaaa                                                                               <  2:989777/140‑1 (MQ=255)                                                              cGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTggg                                                                      >  1:95941/1‑139 (MQ=255)                                                               gCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTg                                                                     <  1:1214396/140‑1 (MQ=255)                                                                  ggAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCgg                                                                  <  1:423536/140‑1 (MQ=255)                                                                            gAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTg                                                        <  1:1301198/140‑1 (MQ=255)                                                                              aCGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCt                                                      <  1:1002668/140‑1 (MQ=255)                                                                                 cgcTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTa                                                   >  1:874906/1‑140 (MQ=255)                                                                                      ttgttgCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGc                                              >  1:964265/1‑140 (MQ=255)                                                                                      ttgttgCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGc                                              >  1:383628/1‑140 (MQ=255)                                                                                                 cTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAaga                                   >  2:775378/1‑140 (MQ=255)                                                                                                 cTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAaga                                   >  2:554869/1‑140 (MQ=255)                                                                                                       aTTGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCa                             <  1:231567/140‑1 (MQ=255)                                                                                                         tGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAag                                    >  1:238610/1‑131 (MQ=255)                                                                                                         tGCCGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAag                                    <  2:238610/131‑1 (MQ=255)                                                                                                           ccGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGgcg                         >  1:927451/1‑140 (MQ=255)                                                                                                            cGCAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGgcgt                        >  1:837075/1‑140 (MQ=255)                                                                                                              cAGATTTAAACATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGgcgtgc                      <  1:554869/140‑1 (MQ=255)                                                                                                                      aaCATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGTACACGCGAAGAGATGCAGGCGTGCGTGGCGCa              <  2:1171144/140‑1 (MQ=255)                                                                                                                      aaCATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCa              <  2:40788/140‑1 (MQ=255)                                                                                                                      aaCATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCa              <  1:1290522/140‑1 (MQ=255)                                                                                                                      aaCATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCa              <  1:65378/140‑1 (MQ=255)                                                                                                                       aCATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCAt             <  2:874906/140‑1 (MQ=255)                                                                                                                       aCATGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCAt             <  2:964265/140‑1 (MQ=255)                                                                                                                          tGCCGGTATTTATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGa          <  1:429690/140‑1 (MQ=255)                                                                                                                                  tttATGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTatat  <  2:682410/140‑1 (MQ=255)                                                                                                                                     aTGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCa              >  1:698789/1‑125 (MQ=255)                                                                                                                                     aTGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCa              <  2:698789/125‑1 (MQ=255)                                                                                                                                      tGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCa              <  2:1211564/124‑1 (MQ=255)                                                                                                                                      tGCGCTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCa              >  1:1211564/1‑124 (MQ=255)                                                                                                                                         |                                                                                                                                     GCGCAGCCAGAAGTGGTGGCGATTGGTGAATGTGGTCTCGACTTTAACCGCAACTTTTCGACGCCGGAAGAGCAGGAACGCGCTTTTGTTGCCCAGCTACGCATTGCCGCAGATTTAAACATGCCGGTATTTATGCACTGTCGCGATGCCCACGAGCGGTTTATGACATTGCTGGAGCCGTGGCTGGATAAACTGCCTGGTGCGGTTCTTCATTGCTTTACCGGCACACGCGAAGAGATGCAGGCGTGCGTGGCGCATGGAATTTATAT  >  NC_000913/4023797‑4024065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑