Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,047,025	C→T	R20R (CGC→CGT)	polA →	DNA polymerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,047,025	0	C	T	100.0%	333.6 / NA	94	R20R (CGC→CGT)	polA	DNA polymerase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (52/42);  total (52/42)

TGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGCGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCGGTGGTCTTTGACGCCAAG  >  NC_000913/4046896‑4047160                                                                                                                                  |                                                                                                                                        tGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCAcg                                                                                                                               >  2:1215228/1‑140 (MQ=255)       aCTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTc                                                                                                                         <  1:1190036/140‑1 (MQ=255)          cATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTcccc                                                                                                                      <  2:1314481/140‑1 (MQ=255)          cATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTcccc                                                                                                                      <  2:142568/140‑1 (MQ=255)          cATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTcccc                                                                                                                      <  1:498427/140‑1 (MQ=255)            tGTTTTCATCCTGTCTGTGGCATCCTTTACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCg                                                                                                                    <  1:1067553/140‑1 (MQ=255)            tGTTTTCATCCTGTCTGTGGCATCCTTTACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCg                                                                                                                    >  2:1173784/1‑140 (MQ=255)            tGTTTTCATCCTGTCTGTGGCATCCTTTACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCg                                                                                                                    <  2:103449/140‑1 (MQ=255)            tGTTTTCATCCTGTCTGTGGCATCCTTTACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCg                                                                                                                    <  1:634951/140‑1 (MQ=255)                        gtctgtGGCATCCTTTACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAgc                                                                                                        >  1:533025/1‑140 (MQ=255)                           tgtgGCATCCTTTACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCa                                                                                                     >  2:526802/1‑140 (MQ=255)                                  tCCTTTACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGc                                                                                              >  1:237410/1‑140 (MQ=255)                                   ccTTTACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGcc                                                                                             >  2:971723/1‑140 (MQ=255)                                   ccTTTACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGcc                                                                                             >  2:626416/1‑140 (MQ=255)                                     tttACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGa                                                                                           <  1:350220/140‑1 (MQ=255)                                     tttACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGa                                                                                           >  2:342060/1‑140 (MQ=255)                                      ttACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGAc                                                                                          <  1:798083/140‑1 (MQ=255)                                       tACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGAcc                                                                                         <  1:1332604/140‑1 (MQ=255)                                          ccATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGt                                                                                      <  2:1001996/140‑1 (MQ=255)                                              aaTCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGa                                                                                  >  1:597427/1‑140 (MQ=255)                                              aaTCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGa                                                                                  >  1:651032/1‑140 (MQ=255)                                              aaTCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGa                                                                                  >  2:304901/1‑140 (MQ=255)                                              aaTCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGa                                                                                  >  2:155345/1‑140 (MQ=255)                                              aaTCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGa                                                                                  >  2:600113/1‑140 (MQ=255)                                                    aTAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCg                                                                                        >  1:134708/1‑128 (MQ=255)                                                    aTAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCg                                                                                        <  2:134708/128‑1 (MQ=255)                                                    aTAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATg                                                                            <  1:1148774/140‑1 (MQ=255)                                                    aTAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATg                                                                            <  1:68842/140‑1 (MQ=255)                                                    aTAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATg                                                                            >  1:504894/1‑140 (MQ=255)                                                      aaaCAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGgt                                                                          <  2:522341/140‑1 (MQ=255)                                                        aCAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGgtgt                                                                        <  1:971723/140‑1 (MQ=255)                                                          aGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTcc                                                                      >  2:544214/1‑140 (MQ=255)                                                           ggCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCt                                                                     >  1:1270376/1‑140 (MQ=255)                                                            gCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATg                                                                            <  1:553191/132‑1 (MQ=255)                                                            gCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATg                                                                            >  2:553191/1‑132 (MQ=255)                                                            gCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTc                                                                    >  2:959808/1‑140 (MQ=255)                                                            gCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTc                                                                    >  1:1398623/1‑140 (MQ=255)                                                            gCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTc                                                                    >  1:941332/1‑140 (MQ=255)                                                             cACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCa                                                                   >  1:1163902/1‑140 (MQ=255)                                                              aCGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCaa                                                                  >  2:1078409/1‑140 (MQ=255)                                                                 gACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACAt                                                               <  1:304901/140‑1 (MQ=255)                                                                    aTTATGGTTCCGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACAtgct                                                            <  1:959808/140‑1 (MQ=255)                                                                     ttATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACAtgctg                                                           <  2:533025/140‑1 (MQ=255)                                                                     ttATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACAtgctg                                                           <  1:876060/140‑1 (MQ=255)                                                                       aTGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTgcg                                                         <  1:1173784/140‑1 (MQ=255)                                                                       aTGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTgcg                                                         <  1:888313/140‑1 (MQ=255)                                                                          gTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAg                                                      >  2:24934/1‑140 (MQ=255)                                                                          gTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAg                                                      >  1:1106380/1‑140 (MQ=255)                                                                          gTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAg                                                      <  1:1215228/140‑1 (MQ=255)                                                                           ttCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGt                                                     >  2:580267/1‑140 (MQ=255)                                                                            tCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACAtgctg                                                           >  1:686581/1‑133 (MQ=255)                                                                            tCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACAtgctg                                                           <  2:686581/133‑1 (MQ=255)                                                                               gATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGa                                                 <  2:780333/140‑1 (MQ=255)                                                                               gATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGa                                                 <  1:544214/140‑1 (MQ=255)                                                                                    cccAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATg                                            <  1:24934/140‑1 (MQ=255)                                                                                    cccAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATg                                            >  2:395471/1‑140 (MQ=255)                                                                                    cccAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATg                                            <  1:476755/140‑1 (MQ=255)                                                                                    cccAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATg                                            <  1:155345/140‑1 (MQ=255)                                                                                    cccAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATg                                            <  1:526802/140‑1 (MQ=255)                                                                                    cccAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATg                                            <  1:1078409/140‑1 (MQ=255)                                                                                     ccAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTTACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGc                                           >  1:1215101/1‑140 (MQ=255)                                                                                     ccAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAAAAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGc                                           >  1:920059/1‑140 (MQ=255)                                                                                        aaaTCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAat                                        >  1:1249254/1‑140 (MQ=255)                                                                                           tCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAAtata                                     <  1:395471/140‑1 (MQ=255)                                                                                                   tCCTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACg                             >  2:1174757/1‑140 (MQ=255)                                                                                                     cTTGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCa                           <  1:342060/140‑1 (MQ=255)                                                                                                       tGTAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATg                         >  2:671043/1‑140 (MQ=255)                                                                                                         tAGATGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCa                       >  2:147331/1‑140 (MQ=255)                                                                                                            aTGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGca                    <  2:504894/140‑2 (MQ=255)                                                                                                             tGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCgg                   <  2:1163902/140‑1 (MQ=255)                                                                                                             tGGTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCgg                   <  1:1048373/140‑1 (MQ=255)                                                                                                              ggTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCggt                  >  1:1349546/1‑140 (MQ=255)                                                                                                              ggTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCggt                  >  2:453370/1‑140 (MQ=255)                                                                                                              ggTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCggt                  >  1:598719/1‑140 (MQ=255)                                                                                                              ggTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCggt                  >  2:1356411/1‑140 (MQ=255)                                                                                                               gTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGcgt                          >  1:1397115/1‑129 (MQ=255)                                                                                                               gTTCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCAt                          <  2:1397115/131‑1 (MQ=255)                                                                                                                ttCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCggt                  >  2:1040238/1‑138 (MQ=255)                                                                                                                ttCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCggt                  <  1:1040238/138‑1 (MQ=255)                                                                                                                ttCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCg                    <  1:499473/136‑1 (MQ=255)                                                                                                                ttCATCTTATCTTTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCg                    >  2:499473/1‑136 (MQ=255)                                                                                                                     cttatcttTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCggt                  >  2:1192553/1‑133 (MQ=255)                                                                                                                     cttatcttTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCggt                  <  1:1192553/133‑1 (MQ=255)                                                                                                                      ttatcttTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCGGTGGTCTTTg          >  2:597263/1‑140 (MQ=255)                                                                                                                        atcttTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCGGTGGTCTTTGAc        >  1:474581/1‑140 (MQ=255)                                                                                                                          cttTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCGGTGGTCTTTGACGc      >  1:1306524/1‑140 (MQ=255)                                                                                                                          cttTATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGAGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCGGTGGTCTTTGACGc      >  1:218391/1‑140 (MQ=255)                                                                                                                            ttATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCGGTGGTCTTTGACGCCa    <  2:237410/140‑1 (MQ=255)                                                                                                                             tATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCGGTGGTCTTTGACGCCaa   >  2:1025307/1‑140 (MQ=255)                                                                                                                             tATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCGGTGGTCTTTGACGCCaa   >  2:737459/1‑140 (MQ=255)                                                                                                                             tATCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGCGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCGCTGGTCTTTGACGCCaa   >  2:806865/1‑140 (MQ=255)                                                                                                                              aTCGTGTATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCGGTGGTCTTTGACGCCAAg  >  2:1350923/1‑140 (MQ=255)                                                                                                                              aTCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCGGTGGTCTTTGACGCCAAg  >  1:745164/1‑140 (MQ=255)                                                                                                                              aTCGTGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCGGTGGTCTTTGACGCCAAg  >  2:74888/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                        TGAAAAACTCATGTTTTCATCCTGTCTGTGGCATCCTTTACCCATAATCTGATAAACAGGCACGGACATTATGGTTCAGATCCCCCAAAATCCACTTATCCTTGTAGATGGTTCATCTTATCTTTATCGCGCATATCACGCGTTTCCCCCGCTGACTAACAGCGCAGGCGAGCCGACCGGTGCGATGTATGGTGTCCTCAACATGCTGCGCAGTCTGATCATGCAATATAAACCGACGCATGCAGCGGTGGTCTTTGACGCCAAG  >  NC_000913/4046896‑4047160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑