Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,055,093 C→T G62G (GGG→GGA)  glnG ← DNA‑binding transcriptional dual regulator NtrC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,055,0930CT100.0% 330.1 / NA 92G62G (GGG→GGA) glnGDNA‑binding transcriptional dual regulator NtrC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (50/42);  total (50/42)

AACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCAC  >  NC_000913/4054962‑4055227
                                                                                                                                   |                                                                                                                                      
aaCGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCAt                                                                                                                                >  1:564469/1‑140 (MQ=255)
 aCGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCAtt                                                                                                                               >  1:890762/1‑140 (MQ=255)
 aCGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCAtt                                                                                                                               >  1:1044155/1‑140 (MQ=255)
   ggTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTcc                                                                                                                             <  1:402380/140‑1 (MQ=255)
   ggTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTcc                                                                                                                             <  2:30649/140‑1 (MQ=255)
     tttGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCg                                                                                                                           >  1:1000083/1‑140 (MQ=255)
     tttGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCg                                                                                                                           >  1:949348/1‑140 (MQ=255)
       tGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGc                                                                                                                         <  1:69797/140‑1 (MQ=255)
       tGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGc                                                                                                                         <  2:1000083/140‑1 (MQ=255)
            aGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACg                                                                                                                    <  2:287284/140‑1 (MQ=255)
            aGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACg                                                                                                                    <  1:1344733/140‑1 (MQ=255)
            aGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACg                                                                                                                    <  1:488840/140‑1 (MQ=255)
              aTAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGa                                                                                                                  >  1:913489/1‑140 (MQ=255)
              aTAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGa                                                                                                                  <  1:679141/140‑1 (MQ=255)
              aTAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGa                                                                                                                  <  2:612317/140‑1 (MQ=255)
              aTAATCAAACGCCCCTTGTTGATAGGCGCTGACGACAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGa                                                                                                                  <  2:10899/140‑1 (MQ=255)
                    aaaCGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTg                                                                                                            >  2:998209/1‑140 (MQ=255)
                        gCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACg                                                                                                                    >  2:1064351/1‑128 (MQ=255)
                        gCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACg                                                                                                                    <  1:1064351/128‑1 (MQ=255)
                        gCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAag                                                                                                        >  2:1026780/1‑140 (MQ=255)
                             ttgttgATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAgcagca                                                                                                   >  1:626680/1‑140 (MQ=255)
                                  gATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATcc                                                                                              >  2:551103/1‑140 (MQ=255)
                                    tAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCgg                                                                                            >  2:74262/1‑140 (MQ=255)
                                    tAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCgg                                                                                            >  2:951341/1‑140 (MQ=255)
                                    tAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCgg                                                                                            >  1:967202/1‑140 (MQ=255)
                                      ggCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAagcagc                                                                                                    >  1:82914/1‑130 (MQ=255)
                                      ggCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAagcagc                                                                                                    <  2:82914/130‑1 (MQ=255)
                                      ggCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCg                                                                                          >  1:283300/1‑140 (MQ=255)
                                       gcgcTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGt                                                                                         >  1:1068831/1‑140 (MQ=255)
                                        cgcTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGtt                                                                                        >  2:1133875/1‑140 (MQ=255)
                                          cTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGtttt                                                                                      >  1:290117/1‑140 (MQ=255)
                                               ggCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCg                                                                                 <  2:890762/140‑1 (MQ=255)
                                               ggCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCg                                                                                 <  2:1044155/140‑1 (MQ=255)
                                                 cagcaTCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGcc                                                                               >  2:195660/1‑140 (MQ=255)
                                                  agcaTCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCa                                                                              >  2:948034/1‑140 (MQ=255)
                                                  agcaTCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCa                                                                              >  1:362357/1‑140 (MQ=255)
                                                    caTCCAGATCGGAATGTGTGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAgc                                                                            <  2:442107/140‑1 (MQ=255)
                                                    caTCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAgc                                                                            >  1:639922/1‑140 (MQ=255)
                                                    caTCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAgc                                                                            <  1:128818/140‑1 (MQ=255)
                                                       ccAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGcc                                                                         <  1:976499/140‑1 (MQ=255)
                                                       ccAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGcc                                                                         <  1:775899/140‑1 (MQ=255)
                                                       ccAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGcc                                                                         <  2:949348/140‑1 (MQ=255)
                                                           aTCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCa                                                                     >  1:855024/1‑140 (MQ=255)
                                                                aaTGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTc                                                                       <  1:880536/133‑1 (MQ=255)
                                                                aaTGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTc                                                                       >  2:880536/1‑133 (MQ=255)
                                                                    tgCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTg                                                            >  1:1196428/1‑140 (MQ=255)
                                                                     gCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTgc                                                           >  1:860944/1‑140 (MQ=255)
                                                                     gCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTgc                                                           >  2:188416/1‑140 (MQ=255)
                                                                      cGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTgcg                                                          <  2:283300/140‑1 (MQ=255)
                                                                        gTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGcc                                                        >  1:59366/1‑140 (MQ=255)
                                                                          cATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGt                                                      >  2:1301019/1‑140 (MQ=255)
                                                                              atgatgACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGcc                                                        <  2:184544/134‑1 (MQ=255)
                                                                              atgatgACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGcc                                                        >  1:184544/1‑134 (MQ=255)
                                                                              atgatgACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTtctc                                                  <  2:991895/140‑1 (MQ=255)
                                                                              atgatgACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTtctc                                                  <  2:693224/140‑1 (MQ=255)
                                                                                gatgaCCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCaa                                                >  2:362179/1‑140 (MQ=255)
                                                                                   gaCCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAAcg                                             >  2:1077962/1‑140 (MQ=255)
                                                                                   gaCCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAAcg                                             >  1:1320060/1‑140 (MQ=255)
                                                                                   gaCCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAAcg                                             >  2:165297/1‑140 (MQ=255)
                                                                                   gaCCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAAcg                                             <  2:656700/140‑1 (MQ=255)
                                                                                   gaCCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAAcg                                             >  1:642438/1‑140 (MQ=255)
                                                                                   gaCCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAAcg                                             >  1:1102380/1‑140 (MQ=255)
                                                                                     ccGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAAcgtc                                           >  2:123248/1‑140 (MQ=255)
                                                                                     ccGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAAcgtc                                           >  2:496668/1‑140 (MQ=255)
                                                                                     ccGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAAcgtc                                           >  1:648318/1‑140 (MQ=255)
                                                                                       ggAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAAcgtcgt                                         >  2:1115694/1‑140 (MQ=255)
                                                                                       ggAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAAcgtcgt                                         >  1:556181/1‑140 (MQ=255)
                                                                                        gAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTa                                        <  1:528598/140‑1 (MQ=255)
                                                                                        gAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTa                                        <  2:967202/140‑1 (MQ=255)
                                                                                             aTTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGt                                   >  1:257663/1‑140 (MQ=255)
                                                                                             aTTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGt                                   <  1:551103/140‑1 (MQ=255)
                                                                                              ttGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGtt                                  <  1:1026780/140‑1 (MQ=255)
                                                                                              ttGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGtt                                  <  2:290117/140‑1 (MQ=255)
                                                                                               tGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTa                                        >  2:317818/1‑133 (MQ=255)
                                                                                               tGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTa                                        <  1:317818/133‑1 (MQ=255)
                                                                                                   tGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGtt                                  >  2:1261608/1‑135 (MQ=255)
                                                                                                   tGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGtt                                  <  1:1261608/135‑1 (MQ=255)
                                                                                                   tGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAAcc                             >  2:862003/1‑140 (MQ=255)
                                                                                                           tttAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCag                     >  2:129405/1‑140 (MQ=255)
                                                                                                             tAATCTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCagcg                   <  1:796991/140‑1 (MQ=255)
                                                                                                                 cTGCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCagcgagcg               <  1:496668/140‑1 (MQ=255)
                                                                                                                   gCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCa             <  2:257663/140‑1 (MQ=255)
                                                                                                                   gCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCa             <  2:556181/140‑1 (MQ=255)
                                                                                                                   gCTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCa             <  1:26225/140‑1 (MQ=255)
                                                                                                                    cTTGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCAc            <  1:1077962/140‑1 (MQ=255)
                                                                                                                     ttGAGCAGCGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAA‑‑‑AGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTc        >  1:235635/1‑140 (MQ=255)
                                                                                                                        agcagcGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTc        <  1:24393/140‑1 (MQ=255)
                                                                                                                          cagcGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCaa      <  1:1133875/140‑1 (MQ=255)
                                                                                                                           agcGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAg     <  1:951341/140‑1 (MQ=255)
                                                                                                                           agcGCCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAg     <  1:998209/140‑1 (MQ=255)
                                                                                                                             cgcCAGTCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCa   >  2:393106/1‑140 (MQ=255)
                                                                                                                              gcCAGTCCGTCCATTCCCGGCATATGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCAc  >  1:891238/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                                      
AACGGTTTGGGCAGATAATCAAACGCCCCTTGTTGATAGGCGCTGACGGCAGCATCCAGATCGGAATGTGCGGTCATAATGATGACCGGAAGCATTGGATGGCGCTGTTTAATCTGCTTGAGCAGCGCCAGCCCGTCCATTCCCGGCATACGGATATCTGAAAGCAGCACATCCGGCGTTTTGCTCGCCAGCGCCTCCAGCACTTCTGCGCCGTTCTCAAACGTCGTACAGGTTAAACCTGCCCCAGCGAGCGCACGTTCAAGCAC  >  NC_000913/4054962‑4055227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑