BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,116,996	G→A	A76V (GCA→GTA)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,116,996	0	G	A	100.0%	399.0 / NA	110	A76V (GCA→GTA)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (54/56); total (54/56)

Only 100 of 110 total aligned reads displayed.

CTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACA  >  NC_000913/4116859‑4117131

ctAAACCGTCACGTTTTAAATGCTTGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACa                                                                                                                                       >  1:50656/1‑140 (MQ=255)

ctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACa                                                                                                                                       >  1:286543/1‑140 (MQ=255)

  aaaCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAAt                                                                                                                                     <  2:1023828/140‑1 (MQ=255)

   aaCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAAtt                                                                                                                                    >  2:1090636/1‑140 (MQ=255)

   aaCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACACTGGTTCGTAATACCGATAGCTACAAtt                                                                                                                                    >  2:965913/1‑140 (MQ=255)

       gTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATCGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGAt                                                                                                                                <  2:864743/140‑1 (MQ=255)

       gTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGAt                                                                                                                                <  1:1090636/140‑1 (MQ=255)

       gTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGAt                                                                                                                                <  2:830803/140‑1 (MQ=255)

       gTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGAt                                                                                                                                <  1:459097/140‑1 (MQ=255)

       gTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACACTGGTTCGTAATACCGATAGCTACAATTTGAt                                                                                                                                <  1:965913/140‑1 (MQ=255)

       gTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTCTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGAt                                                                                                                                <  2:263767/140‑1 (MQ=255)

        tCACGTTTTGAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATc                                                                                                                               >  2:920798/1‑140 (MQ=255)

        tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATc                                                                                                                               >  1:79163/1‑140 (MQ=255)

        tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATc                                                                                                                               >  1:618023/1‑140 (MQ=255)

        tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATc                                                                                                                               >  2:640183/1‑140 (MQ=255)

        tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATc                                                                                                                               <  1:398762/140‑1 (MQ=255)

        tCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATc                                                                                                                               >  2:1262115/1‑140 (MQ=255)

            gTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGaa                                                                                                                           <  2:896038/140‑1 (MQ=255)

            gTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGaa                                                                                                                           <  2:26950/140‑1 (MQ=255)

                tAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGa                                                                                                                       >  2:1230/1‑140 (MQ=255)

                   aTGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGatat                                                                                                                    <  2:1154815/140‑1 (MQ=255)

                   aTGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGatat                                                                                                                    <  1:1186284/140‑1 (MQ=255)

                      cTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCgg                                                                                                                 >  1:1180540/1‑140 (MQ=255)

                      cTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCgg                                                                                                                 >  1:911104/1‑140 (MQ=255)

                       tCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGc                                                                                                                >  2:751647/1‑140 (MQ=255)

                        cGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCt                                                                                                               <  1:334592/140‑1 (MQ=255)

                               ttCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCa                                                                                                        <  1:400946/140‑1 (MQ=255)

                                tCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCa                                                                                                        <  1:299781/139‑1 (MQ=255)

                                tCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCa                                                                                                        >  2:299781/1‑139 (MQ=255)

                                 cTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGc                                                                                                      <  1:801021/140‑1 (MQ=255)

                                  tGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCa                                                                                                     >  2:864560/1‑140 (MQ=255)

                                   gCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCAc                                                                                                    <  2:915461/140‑1 (MQ=255)

                                   gCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCAc                                                                                                    <  1:130658/140‑1 (MQ=255)

                                      gTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTc                                                                                                 <  2:544743/140‑1 (MQ=255)

                                        acgacgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTa                                                                                               <  1:108488/140‑1 (MQ=255)

                                         cgacgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAc                                                                                              <  2:911104/140‑1 (MQ=255)

                                           acgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCa                                                                                            <  1:1154755/140‑1 (MQ=255)

                                            cgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAc                                                                                              <  1:76349/137‑1 (MQ=255)

                                            cgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTAc                                                                                              >  2:76349/1‑137 (MQ=255)

                                               cACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGt                                                                                        <  1:864560/140‑1 (MQ=255)

                                                    ccAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTg                                                                                       >  2:1302928/1‑136 (MQ=255)

                                                    ccAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTg                                                                                       <  1:1302928/136‑1 (MQ=255)

                                                          aaTGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTggg                                                                             <  1:1166760/140‑1 (MQ=255)

                                                            tGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTg                                                                           <  2:1078969/140‑1 (MQ=255)

                                                            tGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTg                                                                           >  1:504618/1‑140 (MQ=255)

                                                                   atagatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGa                                                                    >  1:1091188/1‑140 (MQ=255)

                                                                         agGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCa                                                              >  1:656155/1‑140 (MQ=255)

                                                                         agGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCa                                                              >  1:114793/1‑140 (MQ=255)

                                                                         agGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCa                                                              >  1:32762/1‑140 (MQ=255)

                                                                         agGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTACTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCa                                                              >  2:1392724/1‑140 (MQ=255)

                                                                          ggCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAt                                                             >  1:769697/1‑140 (MQ=255)

                                                                           gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAtt                                                            <  1:1230/140‑1 (MQ=255)

                                                                           gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAtt                                                            >  1:847528/1‑140 (MQ=255)

                                                                           gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAtt                                                            >  1:152496/1‑140 (MQ=255)

                                                                           gCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCAtt                                                            >  1:475522/1‑140 (MQ=255)

                                                                               gCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGt                                                        >  2:989606/1‑140 (MQ=255)

                                                                               gCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCACATTTCCATTGGGt                                                        >  2:1319733/1‑140 (MQ=255)

                                                                                ccGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCATATTTCCATTGGGTc                                                       <  2:618023/140‑1 (MQ=255)

                                                                                ccGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                       >  2:830136/1‑140 (MQ=255)

                                                                                ccGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                       <  2:769697/140‑1 (MQ=255)

                                                                                ccGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                       <  2:656155/140‑1 (MQ=255)

                                                                                ccGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                       <  1:814716/140‑1 (MQ=255)

                                                                                ccGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                       <  1:989606/140‑1 (MQ=255)

                                                                                ccGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGATACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTc                                                       >  1:91025/1‑140 (MQ=255)

                                                                                 cGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCg                                                      >  2:109716/1‑140 (MQ=255)

                                                                                    tttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt                                                   <  1:154312/140‑1 (MQ=255)

                                                                                     ttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATATCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGtt                                                  <  1:687905/140‑1 (MQ=255)

                                                                                     ttCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGtt                                                  <  1:818070/140‑1 (MQ=255)

                                                                                         ttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCACATTTCCATTGGGTCGTGTTCTAc                                              <  1:1319733/140‑1 (MQ=255)

                                                                                          tttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAcc                                             <  1:1365754/140‑1 (MQ=255)

                                                                                          tttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAcc                                             >  2:1092893/1‑140 (MQ=255)

                                                                                              ccAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAAc                                         <  1:1374953/140‑1 (MQ=255)

                                                                                                aGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCt                                       >  2:1350250/1‑140 (MQ=255)

                                                                                                aGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCt                                       >  2:749084/1‑140 (MQ=255)

                                                                                                aGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCt                                       >  2:667712/1‑140 (MQ=255)

                                                                                                    aaTAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtt                                   <  1:480187/140‑1 (MQ=255)

                                                                                                    aaTAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGtt                                   <  1:335750/140‑1 (MQ=255)

                                                                                                              tCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAt                         >  2:1385409/1‑140 (MQ=255)

                                                                                                              tCACGCTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGAt                         >  2:1140064/1‑140 (MQ=255)

                                                                                                                   cTGGTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCt                    <  2:1091188/140‑1 (MQ=255)

                                                                                                                      gTTCGTAATACCGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCaa                 >  2:557395/1‑140 (MQ=255)

                                                                                                                                ccGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTg       >  2:991156/1‑140 (MQ=255)

                                                                                                                                ccGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTg       >  1:662546/1‑140 (MQ=255)

                                                                                                                                ccGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTg       >  1:501763/1‑140 (MQ=255)

                                                                                                                                ccGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTg       >  2:1029506/1‑140 (MQ=255)

                                                                                                                                ccGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTg       >  1:417428/1‑140 (MQ=255)

                                                                                                                                ccGATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTACTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTg       <  1:1392724/140‑1 (MQ=255)

                                                                                                                                  gATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCg     >  1:111806/1‑140 (MQ=255)

                                                                                                                                  gATAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCg     <  1:640183/140‑1 (MQ=255)

                                                                                                                                   aTAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCt                    >  1:340859/1‑124 (MQ=255)

                                                                                                                                   aTAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCt                    <  2:340859/124‑1 (MQ=255)

                                                                                                                                   aTAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa    <  2:152496/140‑1 (MQ=255)

                                                                                                                                   aTAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa    <  2:111806/140‑1 (MQ=255)

                                                                                                                                   aTAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa    <  2:937186/140‑1 (MQ=255)

                                                                                                                                   aTAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa    <  2:504618/140‑1 (MQ=255)

                                                                                                                                   aTAGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCCGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa    <  2:32762/140‑1 (MQ=255)

                                                                                                                                     aGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca  >  1:16843/1‑140 (MQ=255)

                                                                                                                                     aGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca  <  2:475522/140‑1 (MQ=255)

                                                                                                                                     aGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGac   <  2:268421/139‑1 (MQ=255)

                                                                                                                                     aGCTACAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGac   >  1:268421/1‑139 (MQ=255)

CTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACA  >  NC_000913/4116859‑4117131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`