BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,330,680	A→G	D60G (GAC→GGC)	proP →	osmolyte:H(+) symporter ProP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,330,680	0	A	G	100.0%	407.0 / NA	113	D60G (GAC→GGC)	proP	osmolyte:H(+) symporter ProP
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (60/53); total (60/53)

Only 100 of 113 total aligned reads displayed.

ACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCC‑GG‑GGGCTGACCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATCCTCGCTATCACTAT  >  NC_000913/4330547‑4330809

aCCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCa                                                                                                                              >  1:781356/1‑140 (MQ=255)

aCCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCa                                                                                                                              <  1:775932/140‑1 (MQ=255)

aCCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCa                                                                                                                              >  1:1370909/1‑140 (MQ=255)

aCCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCa                                                                                                                              >  2:213647/1‑140 (MQ=255)

      attGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGc                                                                                                                        >  2:1318230/1‑140 (MQ=255)

       ttGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCa                                                                                                                       >  2:1253856/1‑140 (MQ=255)

       ttGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCa                                                                                                                       >  2:501581/1‑140 (MQ=255)

        tgatgaCGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAg                                                                                                                      >  2:1314922/1‑140 (MQ=255)

          atgaCGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGAt                                                                                                                    <  1:266970/140‑1 (MQ=255)

               ggTAAACTGCGTAAAGCCGTTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTtg                                                                                                               <  1:1281115/140‑1 (MQ=255)

               ggTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTtg                                                                                                               >  1:808177/1‑140 (MQ=255)

               ggTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTtg                                                                                                               >  1:177273/1‑140 (MQ=255)

                 tAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTtgct                                                                                                             >  2:1022873/1‑140 (MQ=255)

                  aaaCTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTtgctg                                                                                                            <  2:835474/140‑1 (MQ=255)

                  aaaCTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTtgctg                                                                                                            <  1:1262951/140‑1 (MQ=255)

                  aaaCTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTtgctg                                                                                                            <  1:124033/140‑1 (MQ=255)

                        cGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTg                                                                                                      >  1:809399/1‑140 (MQ=255)

                        cGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTg                                                                                                      >  2:204221/1‑140 (MQ=255)

                         gTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGTAGATGGTTGCTGCACTTGc                                                                                                     >  1:705509/1‑140 (MQ=255)

                          tAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGcc                                                                                                    >  1:1095646/1‑140 (MQ=255)

                              gCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACtt                                                                                                <  1:371242/140‑1 (MQ=255)

                                  ttACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTtctc                                                                                            >  1:180460/1‑140 (MQ=255)

                                  ttACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTAT‑GTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTcc                                                                                           >  1:595957/1‑140 (MQ=255)

                                     ccGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGt                                                                                         <  2:395637/140‑1 (MQ=255)

                                         agcaTCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTccc                                                                                     >  2:1136600/1‑140 (MQ=255)

                                                     tAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGa                                                                         >  2:367771/1‑140 (MQ=255)

                                                     tAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGa                                                                         <  1:1054555/140‑1 (MQ=255)

                                                     tAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGa                                                                         <  2:1095646/140‑1 (MQ=255)

                                                     tAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGa                                                                         >  2:632018/1‑140 (MQ=255)

                                                       aTGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGAcc                                                                       >  1:1079483/1‑140 (MQ=255)

                                                        tGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCg                                                                      >  1:333658/1‑140 (MQ=255)

                                                        tGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCg                                                                      >  1:801394/1‑140 (MQ=255)

                                                        tGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCg                                                                      >  2:66257/1‑140 (MQ=255)

                                                        tGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCg                                                                      >  2:1284426/1‑140 (MQ=255)

                                                        tGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCg                                                                      >  1:1136767/1‑140 (MQ=255)

                                                        tGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCg                                                                      >  2:1046302/1‑140 (MQ=255)

                                                        tGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCAACTTTCTCCGTTCCCTTTCTGATTCGACCg                                                                      >  2:646083/1‑140 (MQ=255)

                                                            atggaatggTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGTAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTg                                                                  <  2:705509/140‑1 (MQ=255)

                                                             tggaatggTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTgg                                                                 >  2:252804/1‑140 (MQ=255)

                                                             tggaatggTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTgg                                                                 >  1:91142/1‑140 (MQ=255)

                                                             tggaatggTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTgg                                                                 <  1:5440/140‑1 (MQ=255)

                                                             tggaatggTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTgg                                                                 >  2:1165895/1‑140 (MQ=255)

                                                             tggaatggTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTgg                                                                 >  1:1330711/1‑140 (MQ=255)

                                                              ggaatggTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGc                                                                <  2:974750/140‑1 (MQ=255)

                                                                  tggTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGAc                                                                        <  2:1045350/128‑1 (MQ=255)

                                                                  tggTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGAc                                                                        >  1:1045350/1‑128 (MQ=255)

                                                                  tggTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGa                                                             >  2:1176828/1‑139 (MQ=255)

                                                                  tggTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGa                                                             <  1:1248466/139‑1 (MQ=255)

                                                                  tggTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGa                                                             <  1:1176828/139‑1 (MQ=255)

                                                                  tggTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGAc                                                            >  2:186438/1‑140 (MQ=255)

                                                                  tggTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGAc                                                            >  2:182780/1‑140 (MQ=255)

                                                                  tggTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGAc                                                            >  2:501089/1‑140 (MQ=255)

                                                                  tggTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCC‑GGAAGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGa                                                             >  2:1248466/1‑139 (MQ=255)

                                                                   ggTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGAct                                                           <  2:808177/140‑1 (MQ=255)

                                                                    gTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGActc                                                          >  2:1055525/1‑140 (MQ=255)

                                                                    gTTCGATTTTGGCGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGActc                                                          >  2:580691/1‑140 (MQ=255)

                                                                     ttCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGAcc                                                                       >  1:791329/1‑126 (MQ=255)

                                                                     ttCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGAcc                                                                       <  2:791329/126‑1 (MQ=255)

                                                                     ttCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGActct                                                         >  1:197728/1‑140 (MQ=255)

                                                                     ttCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGActct                                                         <  2:1370909/140‑1 (MQ=255)

                                                                     ttCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGActct                                                         <  1:1318230/140‑1 (MQ=255)

                                                                     ttCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTCCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCg                                                               <  1:1112157/134‑1 (MQ=255)

                                                                     ttCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTCCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCg                                                               >  2:1112157/1‑134 (MQ=255)

                                                                      tCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACtctt                                                        <  2:592691/140‑1 (MQ=255)

                                                                      tCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACtctt                                                        <  2:91142/140‑1 (MQ=255)

                                                                       cGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACtcttc                                                       >  2:370630/1‑140 (MQ=255)

                                                                       cGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTT‑‑TCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTcttctt                                                     <  2:752970/140‑1 (MQ=255)

                                                                        gATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACtcttct                                                      <  2:1136767/140‑1 (MQ=255)

                                                                        gATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACtcttct                                                      <  1:988568/140‑1 (MQ=255)

                                                                         aTTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTcttctt                                                     >  2:89509/1‑140 (MQ=255)

                                                                                  tttATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGtt                                            <  1:632018/140‑1 (MQ=255)

                                                                                    tATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTgg                                          >  1:1210074/1‑140 (MQ=255)

                                                                                    tATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTgg                                          <  2:197728/140‑1 (MQ=255)

                                                                                       ggTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCg                                       <  1:89509/140‑1 (MQ=255)

                                                                                       ggTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCg                                       <  1:330172/140‑1 (MQ=255)

                                                                                       ggTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCg                                       <  1:367771/140‑1 (MQ=255)

                                                                                         ttttGTTGCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGAt                                     >  1:1019381/1‑140 (MQ=255)

                                                                                             gttcCTTACG‑AATAAGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAata                                <  1:845208/140‑1 (MQ=255)

                                                                                              ttgCTTACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAata                                <  1:1265414/140‑1 (MQ=255)

                                                                                                  ttACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGt                            <  2:140060/140‑1 (MQ=255)

                                                                                                   tACGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTc                           >  1:1218586/1‑140 (MQ=255)

                                                                                                    aCGCATTAGGTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCg                          <  1:501581/140‑1 (MQ=255)

                                                                                                            ggTAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCagaaga                  >  1:1225523/1‑140 (MQ=255)

                                                                                                              tAAAGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATc                <  1:646083/140‑1 (MQ=255)

                                                                                                               aaaGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATcc               <  1:1022873/140‑1 (MQ=255)

                                                                                                                aaGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATCCt              <  1:805099/140‑1 (MQ=255)

                                                                                                                aaGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATCCt              <  2:1141684/140‑1 (MQ=255)

                                                                                                                 aGTTTTTTTCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATCCTc             >  2:1370234/1‑140 (MQ=255)

                                                                                                                   tttttttCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATCCTCGc           <  1:1314922/140‑1 (MQ=255)

                                                                                                                     ttttcCCG‑GG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATCCTCGCTAt        <  2:726515/140‑1 (MQ=255)

                                                                                                                     ttttcCCG‑GG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATCCTCGCTAt        >  2:352756/1‑140 (MQ=255)

                                                                                                                     ttttcCCG‑GG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATCCTCGCTAt        <  2:333658/140‑1 (MQ=255)

                                                                                                                      tttcCCG‑GG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATCCTCGCTATc       <  2:900497/140‑1 (MQ=255)

                                                                                                                       tttCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATCCTCGCTATc       >  1:676350/1‑140 (MQ=255)

                                                                                                                        ttCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATCCTCGCTATCa      <  1:580691/140‑1 (MQ=255)

                                                                                                                        ttCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATCCTCGCTATCa      <  2:801394/140‑1 (MQ=255)

                                                                                                                        ttCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATCCTCGCTATCa      <  2:809399/140‑1 (MQ=255)

                                                                                                                        ttCCCGGG‑GGGCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATCCTCGCTATCa      <  1:204221/140‑1 (MQ=255)

                                                                                                                           ccggg‑gggCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATCCTCGCTATCACTa   <  1:182780/137‑1 (MQ=255)

                                                                                                                            cggg‑gggCTGGCCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATCCTCGCTATCACTAt  <  2:1163214/138‑1 (MQ=255)

ACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAGCATCACTGGGTAATGCAATGGAATGGTTCGATTTTGGTGTTTATGGTTTTGTTGCTTACGCATTAGGTAAAGTTTTTTTCCC‑GG‑GGGCTGACCCCAGCGTGCAGATGGTTGCTGCACTTGCCACTTTCTCCGTTCCCTTTCTGATTCGACCGCTTGGCGGACTCTTCTTTGGTATGTTGGGCGATAAATATGGTCGCCAGAAGATCCTCGCTATCACTAT  >  NC_000913/4330547‑4330809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`