Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,484,372	T→C	intergenic (‑92/+68)	holC ← / ← pepA	DNA polymerase III subunit chi/aminopeptidase A/I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,484,372	0	T	C	100.0%	240.0 / NA	66	intergenic (‑92/+68)	holC/pepA	DNA polymerase III subunit chi/aminopeptidase A/I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/21);  total (45/21)

CGACGGTGGTGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGT  >  NC_000913/4484235‑4484508                                                                                                                                          |                                                                                                                                         cGACGGTGGTGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTc                                                                                                                                        >  2:1119694/1‑140 (MQ=255) cGACGGTGGTGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTc                                                                                                                                        >  1:67832/1‑140 (MQ=255)     ggtggtGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTg                                                                                                                                    <  1:775791/140‑1 (MQ=255)         gtgtCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCtgt                                                                                                                                <  1:1119694/140‑1 (MQ=255)           gtCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTgtgt                                                                                                                              <  1:1216617/140‑1 (MQ=255)               ttGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGt                                                                                                                          >  2:995336/1‑140 (MQ=255)                  tCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTg                                                                                                                                    <  2:1198299/127‑1 (MQ=255)                  tCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTg                                                                                                                                    >  1:1198299/1‑127 (MQ=255)                  tCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTg                                                                                                                                    >  1:55041/1‑127 (MQ=255)                  tCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTg                                                                                                                                    <  2:55041/127‑1 (MQ=255)                    cAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGAt                                                                                                                     >  2:1364882/1‑140 (MQ=255)                      gAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAgg                                                                                                                           >  2:1274738/1‑132 (MQ=255)                      gAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAgg                                                                                                                           <  1:1274738/132‑1 (MQ=255)                            aGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGtt                                                                                                             >  1:924469/1‑140 (MQ=255)                                cGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCAcg                                                                                                         <  1:1312904/140‑1 (MQ=255)                                 gTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCAcgc                                                                                                        >  1:336701/1‑140 (MQ=255)                                   cgcgTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCAcgccg                                                                                                      >  2:290557/1‑140 (MQ=255)                                   cgcgTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCAcgccg                                                                                                      >  1:804478/1‑140 (MQ=255)                                   cgcgTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCAcgccg                                                                                                      >  1:762630/1‑140 (MQ=255)                                   cgcgTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCAcgccg                                                                                                      >  1:748567/1‑140 (MQ=255)                                                       cttcttGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCtt                                                                                  >  2:1211209/1‑140 (MQ=255)                                                       cttcttGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCtt                                                                                  >  2:417097/1‑140 (MQ=255)                                                           ttGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCt                                                                              >  1:331087/1‑140 (MQ=255)                                                             gtggtgGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGa                                                                            <  1:1211209/140‑1 (MQ=255)                                                                            aCGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCg                                                             <  1:1364882/140‑1 (MQ=255)                                                                            aCGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCg                                                             <  1:851981/140‑1 (MQ=255)                                                                             cGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGc                                                            >  1:157030/1‑140 (MQ=255)                                                                             cGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGc                                                            >  2:1337831/1‑140 (MQ=255)                                                                             cGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGc                                                            >  2:621978/1‑140 (MQ=255)                                                                             cGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGc                                                            >  1:338545/1‑140 (MQ=255)                                                                             cGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGc                                                            >  1:456638/1‑140 (MQ=255)                                                                             cGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGc                                                            >  1:312891/1‑140 (MQ=255)                                                                                aTCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGt                                                         <  1:483888/140‑1 (MQ=255)                                                                                  cAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTa                                                       >  1:576119/1‑140 (MQ=255)                                                                                        taCACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCa                                                 >  1:671719/1‑140 (MQ=255)                                                                                        taCACCACAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCa                                                 >  2:366429/1‑140 (MQ=255)                                                                                           acCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAgcg                                              <  2:456638/140‑1 (MQ=255)                                                                                             cGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGcgcg                                            >  1:520844/1‑140 (MQ=255)                                                                                             cGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGcgcg                                            >  2:1307504/1‑140 (MQ=255)                                                                                              gCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAgc                                               >  2:797602/1‑136 (MQ=255)                                                                                              gCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAgc                                               <  1:797602/136‑1 (MQ=255)                                                                                              gCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCgg                                           >  1:873432/1‑140 (MQ=255)                                                                                               cAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGt                                          >  2:60572/1‑140 (MQ=255)                                                                                               cAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGt                                          >  1:764290/1‑140 (MQ=255)                                                                                               cAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGt                                          <  2:150031/140‑1 (MQ=255)                                                                                               cAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGt                                          >  2:818122/1‑140 (MQ=255)                                                                                               cAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGt                                          <  1:417097/140‑1 (MQ=255)                                                                                                  cgcCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTa                                       >  1:948481/1‑140 (MQ=255)                                                                                                  cgcCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTa                                       >  2:948297/1‑140 (MQ=255)                                                                                                    ccTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAAc                                     >  2:1192760/1‑140 (MQ=255)                                                                                                       gATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAgg                                  <  1:397373/140‑1 (MQ=255)                                                                                                           cGACGCTGTTGTGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACt                              <  1:290557/140‑1 (MQ=255)                                                                                                                   ttgtGTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGc                      >  2:828013/1‑140 (MQ=255)                                                                                                                    tgtgTCTTATCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCa                     <  2:157030/140‑1 (MQ=255)                                                                                                                             tCAGGCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCg            >  1:761508/1‑140 (MQ=255)                                                                                                                                 gCCTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGgacg        <  1:60572/140‑1 (MQ=255)                                                                                                                                  ccTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAAc                   >  2:862224/1‑128 (MQ=255)                                                                                                                                  ccTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAAc                   <  1:862224/128‑1 (MQ=255)                                                                                                                                  ccTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGgacga       >  1:405325/1‑140 (MQ=255)                                                                                                                                  ccTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGgacga       >  2:712764/1‑140 (MQ=255)                                                                                                                                  ccTACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGgacga       >  1:1294384/1‑140 (MQ=255)                                                                                                                                    tACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGAcc     >  2:575924/1‑140 (MQ=255)                                                                                                                                    tACGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGAcc     >  2:163333/1‑140 (MQ=255)                                                                                                                                      cGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCgg   >  1:212168/1‑140 (MQ=255)                                                                                                                                      cGAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCgg   >  1:1067129/1‑140 (MQ=255)                                                                                                                                       gAGCTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGt  <  2:336701/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CGACGGTGGTGTCATTGTCCAGAAGGTAGAACGTCGCGTTTTTCATAAATGGGGCTTCTTGTGGTGGATTTAAATACGCATCAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGT  >  NC_000913/4484235‑4484508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑