Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,565,777	A→G	V25A (GTC→GCC)	yjiM ←	putative dehydratase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,565,777	0	A	G	100.0%	332.5 / NA	91	V25A (GTC→GCC)	yjiM	putative dehydratase subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/55);  total (36/55)

TCTCCGCTTCTTCAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGACGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAG  >  NC_000913/4565640‑4565913                                                                                                                                          |                                                                                                                                          tctcCGCTTCTTCAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCg                                                                                                                                         >  1:788659/1‑140 (MQ=255) tctcCGCTTCTTCAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCg                                                                                                                                         >  2:70993/1‑140 (MQ=255)   tcCGCTTCTTCAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGt                                                                                                                                       >  2:439496/1‑140 (MQ=255)      gCTTCTTCAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTgag                                                                                                                                    >  2:1007598/1‑140 (MQ=255)      gCTTCTTCAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTgag                                                                                                                                    <  2:952648/140‑1 (MQ=255)       cttcttCAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTgaga                                                                                                                                   <  2:1104704/140‑1 (MQ=255)        ttcttcAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGaa                                                                                                                                  >  1:1278094/1‑140 (MQ=255)           ttcAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGc                                                                                                                               >  2:988418/1‑140 (MQ=255)            tcAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGcc                                                                                                                              <  1:1007598/140‑1 (MQ=255)               aTGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGt                                                                                                                           >  1:798827/1‑140 (MQ=255)               aTGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGt                                                                                                                           <  2:640289/140‑1 (MQ=255)                    ttCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGc                                                                                                                      >  2:62500/1‑140 (MQ=255)                    ttCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGc                                                                                                                      <  2:549699/140‑1 (MQ=255)                       atcaGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAg                                                                                                                   <  2:779740/140‑1 (MQ=255)                       atcaGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAg                                                                                                                   <  1:810867/140‑1 (MQ=255)                       atcaGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAg                                                                                                                   <  1:1084025/140‑1 (MQ=255)                            agGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCa                                                                                                              >  1:1027111/1‑140 (MQ=255)                            agGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCa                                                                                                              <  2:951972/140‑1 (MQ=255)                            agGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGAGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGGGAGAAAGCCTGTCTGGCGAGCTTCa                                                                                                              >  2:591315/1‑140 (MQ=255)                             ggTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCag                                                                                                             >  1:1068116/1‑140 (MQ=255)                             ggTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCag                                                                                                             >  1:474195/1‑140 (MQ=255)                              gTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCaga                                                                                                            <  1:947114/140‑1 (MQ=255)                              gTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCaga                                                                                                            >  2:1209471/1‑140 (MQ=255)                              gTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCaga                                                                                                            >  1:646768/1‑140 (MQ=255)                              gTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCaga                                                                                                            >  1:1084594/1‑140 (MQ=255)                               tGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCagag                                                                                                           <  2:539923/140‑1 (MQ=255)                                 gAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGaa                                                                                                         <  2:795214/140‑1 (MQ=255)                                 gAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGaa                                                                                                         >  1:694683/1‑140 (MQ=255)                                 gAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGaa                                                                                                         >  2:906076/1‑140 (MQ=255)                                 gAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGaa                                                                                                         <  2:656845/140‑1 (MQ=255)                                      gagCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGAt                                                                                                    >  2:134364/1‑140 (MQ=255)                                      gagCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGAt                                                                                                    >  2:970399/1‑140 (MQ=255)                                      gagCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGAt                                                                                                    >  1:775477/1‑140 (MQ=255)                                       agCGAAACCACAACCGCACCGACTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATc                                                                                                   >  2:993564/1‑140 (MQ=255)                                        gCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCg                                                                                                  >  1:345307/1‑140 (MQ=255)                                        gCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCg                                                                                                  >  2:102050/1‑140 (MQ=255)                                        gCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCg                                                                                                  >  2:1001155/1‑140 (MQ=255)                                        gCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCg                                                                                                  >  1:1020783/1‑140 (MQ=255)                                        gCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCg                                                                                                  >  2:977660/1‑140 (MQ=255)                                         cGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGa                                                                                                 >  2:621048/1‑140 (MQ=255)                                         cGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGa                                                                                                 >  2:865855/1‑140 (MQ=255)                                           aaaCCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGaaa                                                                                               <  2:694683/140‑1 (MQ=255)                                            aaCCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGaaaa                                                                                              >  1:489849/1‑140 (MQ=255)                                              ccACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATg                                                                                            <  1:906076/140‑1 (MQ=255)                                              ccACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATg                                                                                            <  1:1243487/140‑1 (MQ=255)                                                   aCCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCggg                                                                                       >  2:824410/1‑140 (MQ=255)                                                   aCCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCggg                                                                                       <  2:94908/140‑1 (MQ=255)                                                    ccGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGt                                                                                      <  1:977660/140‑1 (MQ=255)                                                     cGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTa                                                                                     <  1:11231/140‑1 (MQ=255)                                                       cACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGa                                                                                   <  1:70993/140‑1 (MQ=255)                                                           ggCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCgg                                                                               <  1:62500/140‑1 (MQ=255)                                                            gCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGt                                                                              <  2:244108/140‑1 (MQ=255)                                                               gCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGAc                                                                           <  2:1068116/140‑1 (MQ=255)                                                               gCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGAc                                                                           <  1:330011/140‑1 (MQ=255)                                                               gCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGAc                                                                           <  2:1020783/140‑1 (MQ=255)                                                                       gATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACa                                                                   <  2:798827/140‑1 (MQ=255)                                                                               gCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTttc                                                          <  2:688601/140‑1 (MQ=255)                                                                                cGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGGAGATCGGGGACAAGTGACAAGA‑tttttc                                                         >  1:138162/1‑138 (MQ=255)                                                                                     tAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTccc                                                    <  2:738075/140‑1 (MQ=255)                                                                                       aaGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCg                                                  <  2:611153/140‑1 (MQ=255)                                                                                       aaGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCg                                                  <  1:93270/140‑1 (MQ=255)                                                                                         ggTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTa                                                <  2:489849/140‑1 (MQ=255)                                                                                            gCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCa                                             <  1:1133992/140‑1 (MQ=255)                                                                                              aGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACg                                           <  2:775477/140‑1 (MQ=255)                                                                                                  aGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGgag                                       <  1:42742/140‑1 (MQ=255)                                                                                                  aGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGGAGATCGGGGGCAAAAGACATGA‑TTTTTCTTTCCCCCACCACggggg                                       >  2:42742/1‑138 (MQ=255)                                                                                                  aGTGCCAACCAGCGGAATGCCGAGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGGAGATCGGGGGCAAGGGACATGA‑TTTTTCTTCCCCCTACCACcgggg                                       >  2:89263/1‑138 (MQ=255)                                                                                                    tGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGGCGATTGGGGGCAAGGGGAAAGAGTTTTCTTTCCCCTACCCACGGggga                                      >  2:1098616/1‑140 (MQ=255)                                                                                                       cAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAAt                                  <  2:812489/140‑1 (MQ=255)                                                                                                       cAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACACGA‑TTTTCCTTCCCCGTACCACGGGAGATAAt                                  <  2:1278094/140‑1 (MQ=255)                                                                                                           cAGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGaaa                              <  1:667030/140‑1 (MQ=255)                                                                                                            aGCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGaaaa                             <  1:455250/140‑1 (MQ=255)                                                                                                             gCGGAATGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAag                            <  1:1098616/140‑1 (MQ=255)                                                                                                                 aaTGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGc                        <  1:824410/140‑1 (MQ=255)                                                                                                                 aaTGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGc                        <  1:1001155/140‑1 (MQ=255)                                                                                                                 aaTGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGc                        <  2:407643/140‑1 (MQ=255)                                                                                                                 aaTGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGc                        <  2:1027111/140‑1 (MQ=255)                                                                                                                 aaTGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGc                        <  1:716063/140‑1 (MQ=255)                                                                                                                 aaTGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGc                        <  1:865855/140‑1 (MQ=255)                                                                                                                  aTGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGcc                       <  1:465318/140‑1 (MQ=255)                                                                                                                  aTGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGcc                       <  2:345307/140‑1 (MQ=255)                                                                                                                   tGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCca                      <  1:621048/140‑1 (MQ=255)                                                                                                                   tGCCGCGCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCca                      <  1:993564/140‑1 (MQ=255)                                                                                                                      cgcgCTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACat                   <  1:134364/140‑1 (MQ=255)                                                                                                                        cgcTCCTTGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGGAAATTTGGGGGAAAGGACATGA‑TTTTTCTTCCCCCCCCCACGGGAGATAATGAAAAGAGGGCACcttt                 >  2:716063/1‑139 (MQ=255)                                                                                                                              ttGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCa           <  1:325711/140‑1 (MQ=255)                                                                                                                              ttGAGATCCATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCa           <  2:474195/140‑1 (MQ=255)                                                                                                                                     ccATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCtttt    <  2:646768/140‑1 (MQ=255)                                                                                                                                     ccATGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCtttt    <  2:1084594/140‑1 (MQ=255)                                                                                                                                       aTGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAg  <  1:518089/140‑1 (MQ=255)                                                                                                                                       aTGGCGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGGAGAGCGGTGAAAAGAGGCATGA‑TTTTTCTTCCCCCCTTCACCCGAGGTAATAAAAAAAGGGGACAGTTTTACCATtttttttt  >  2:73318/1‑138 (MQ=255)                                                                                                                                          |                                                                                                                                          TCTCCGCTTCTTCAATGGTTTCATCAGAGGTGGAACAGAGCGAAACCACAACCGCACCGGCTGCCATCGGGATCTCTTGCGGCATAAAGGTGCAGTAAGTGCCAACCAGCGGAATGCCGCGCTCCTTGAGATCCATGACGGTGAGAAAGCCTGTCTGGCGAGCTTCAGAGAACTGATCGAAAATGGCGGGTAGATCGGTGACAAGTGACATGA‑TTTTCCTTCCCCGTACCACGGGAGATAATGAAAAGAGGCCACATTATTACCATTCTTTTAG  >  NC_000913/4565640‑4565913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑