Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	103,073	A→G	S281G (AGC→GGC)	ddlB →	D‑alanine‑‑D‑alanine ligase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	103,073	0	A	G	100.0%	206.9 / NA	58	S281G (AGC→GGC)	ddlB	D‑alanine‑‑D‑alanine ligase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/33);  total (25/33)

CAATTTGCAGGCATTAGTGCTGAAAGCATGGACGACGTTAGGTTGCAAAGGATGGGGACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACAGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGTTTCTTCTCGC  >  NC_000913/102937‑103208                                                                                                                                         |                                                                                                                                        cAATTTGCAGGCATTAGTGCTGAAAGCATGGACGACGTTAGGTTGCAAAGGATGGGGACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGcc                                                                                                                                      >  2:1257460/1‑140 (MQ=255)   aTTTGCAGGCATTAGTGCTGAAAGCATGGACGACGTTAGGTTGCAAAGGATGGGGACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTg                                                                                                                                    <  1:797857/140‑1 (MQ=255)               tAGTGCTGAAAGCATGGACGACGTTAGGTTGCAAAGGATGGGGACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATggcg                                                                                                                        <  2:1185066/140‑1 (MQ=255)                        aaGCATGGACGACGTTAGGTTGCAAAGGATGGGGACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTcag                                                                                                               <  2:1384092/140‑1 (MQ=255)                         aGCATGGACGACGTTAGGTTGCAAAGGATGGGGACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTcagg                                                                                                              <  1:731017/140‑1 (MQ=255)                            aTGGACGACGTTAGGTTGCAAAGGATGGGGACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTcaggcag                                                                                                           >  1:355292/1‑140 (MQ=255)                            aTGGACGACGTTAGGTTGCAAAGGATGGGGACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTcaggcag                                                                                                           >  2:162837/1‑140 (MQ=255)                                 cgacgTTAGGTTGCAAAGGATGGGGACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATg                                                                                                      <  1:1257460/140‑1 (MQ=255)                                         ggTTGCAAAGGATGGGGACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTGTGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTtctc                                                                                              <  2:254924/140‑1 (MQ=255)                                              cAAAGGATGGGGACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGt                                                                                         >  2:386526/1‑140 (MQ=255)                                              cAAAGGATGGGGACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGt                                                                                         <  2:257335/140‑1 (MQ=255)                                              cAAAGGATGGGGACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGt                                                                                         >  1:148846/1‑140 (MQ=255)                                              cAAAGGATGGGGACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGt                                                                                         <  1:386526/140‑1 (MQ=255)                                              cAAAGGATGGGGACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGt                                                                                         >  1:257335/1‑140 (MQ=255)                                               aaaGGATGGGGACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGtt                                                                                        >  2:732455/1‑140 (MQ=255)                                                  ggATGGGGACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGgt                                                                                     <  1:1198065/140‑1 (MQ=255)                                                   gATGGGGACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGgta                                                                                    >  1:567408/1‑140 (MQ=255)                                                       gggACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGCGCTTCTCGCAGTTGGTAGTAc                                                                                <  2:991849/140‑1 (MQ=255)                                                        ggACGTATTGACGTTATGCTGGATAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACg                                                                               <  2:938748/140‑1 (MQ=255)                                                             tATTGACGTTATGCTGGACAGCGATGGACAGTTTTATGTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTc                                                                          >  1:254401/1‑140 (MQ=255)                                                             tATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTc                                                                          >  2:634057/1‑140 (MQ=255)                                                             tATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTc                                                                          >  2:617966/1‑140 (MQ=255)                                                             tATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTc                                                                          >  2:853602/1‑140 (MQ=255)                                                                tGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTgg                                                                       <  2:256631/140‑1 (MQ=255)                                                                  aCGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGaa                                                                     <  1:175058/140‑1 (MQ=255)                                                                      tATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTgg                                                                 <  1:1164751/140‑1 (MQ=255)                                                                        tGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCg                                                               <  1:245623/140‑1 (MQ=255)                                                                        tGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCg                                                               >  1:409769/1‑140 (MQ=255)                                                                               cAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAat                                                        <  2:526121/140‑1 (MQ=255)                                                                               cAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAat                                                        >  2:130337/1‑140 (MQ=255)                                                                               cAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAat                                                        <  2:567408/140‑1 (MQ=255)                                                                               cAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAat                                                        <  1:162837/140‑1 (MQ=255)                                                                                  cGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATg                                                     >  2:88997/1‑140 (MQ=255)                                                                                  cGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATg                                                     >  1:1351139/1‑140 (MQ=255)                                                                                       gACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCa                                                <  1:744792/140‑1 (MQ=255)                                                                                         cAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAgg                                              <  1:1161815/140‑1 (MQ=255)                                                                                         cAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAgg                                              <  1:634057/140‑1 (MQ=255)                                                                                         cAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCAATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAgg                                              <  2:81358/140‑1 (MQ=255)                                                                                             tttATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGgctgc                                          <  1:88997/140‑1 (MQ=255)                                                                                              ttATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGgctgct                                         <  1:881198/140‑1 (MQ=255)                                                                                              ttATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGgctgct                                         <  2:181968/140‑1 (MQ=255)                                                                                                 tCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTg                                      >  2:1328163/1‑140 (MQ=255)                                                                                                  ctgctgGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGa                                     <  2:198830/140‑1 (MQ=255)                                                                                                  ctgctgGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGa                                     <  1:1328163/140‑1 (MQ=255)                                                                                                      tgGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGACCTGGCGGACTAATATGTCGCAGGCTGCTCTGAacac                                 <  2:1037430/140‑1 (MQ=255)                                                                                                      tgGAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAacac                                 >  1:1037430/1‑140 (MQ=255)                                                                                                        gAAGCCAATACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACAcgc                               >  1:1301112/1‑140 (MQ=255)                                                                                                               aTACCTCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAg                        <  1:42664/140‑1 (MQ=255)                                                                                                                    tCACCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCgaag                   >  2:1094857/1‑140 (MQ=255)                                                                                                                      aCCGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTGTGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCgaagaa                 >  1:844778/1‑140 (MQ=255)                                                                                                                       ccGGGTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGaagaag                <  2:409769/140‑1 (MQ=255)                                                                                                                          ggTATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAgg             >  2:202293/1‑140 (MQ=255)                                                                                                                            tATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGtt           >  1:389435/1‑140 (MQ=255)                                                                                                                            tATGACCAGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGtt           >  1:772751/1‑140 (MQ=255)                                                                                                                                   aGCCACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGTTTCTtctc    <  1:1094857/140‑1 (MQ=255)                                                                                                                                     ccACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGTTTCTTCTcgc  <  2:355292/140‑1 (MQ=255)                                                                                                                                     ccACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGTTTCTTCTcgc  >  1:117338/1‑140 (MQ=255)                                                                                                                                     ccACGGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGTTTCTTCTcgc  <  1:1119872/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CAATTTGCAGGCATTAGTGCTGAAAGCATGGACGACGTTAGGTTGCAAAGGATGGGGACGTATTGACGTTATGCTGGACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCACAGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGAATTCTGGAACTGGCGGACTAATATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGTTTCTTCTCGC  >  NC_000913/102937‑103208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑