Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	842,550	C→T	D73D (GAC→GAT)	rlmF →	23S rRNA m(6)A1618 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	842,550	0	C	T	100.0%	237.4 / NA	67	D73D (GAC→GAT)	rlmF	23S rRNA m(6)A1618 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/40);  total (27/40)

CCTGAACTCAGGCAATTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTG  >  NC_000913/842413‑842678                                                                                                                                          |                                                                                                                                 ccTGAACTCAGGCAATTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATgg                                                                                                                                >  2:49740/1‑140 (MQ=255)     aaCTCAGGCAATTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGtttt                                                                                                                            >  2:1068748/1‑140 (MQ=255)     aaCTCAGGCAATTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGtttt                                                                                                                            >  1:1382432/1‑140 (MQ=255)     aaCTCAGGCAATTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGtttt                                                                                                                            <  1:1320873/140‑1 (MQ=255)     aaCTCAGGCAATTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGtttt                                                                                                                            <  1:1068748/140‑1 (MQ=255)      aCTCAGGCAATTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTtc                                                                                                                           >  2:125923/1‑140 (MQ=255)       cTCAGGCAATTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTtct                                                                                                                          <  2:1216390/140‑1 (MQ=255)          aGGCAATTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTg                                                                                                                       <  2:291664/140‑1 (MQ=255)          aGGCAATTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTg                                                                                                                       >  1:291664/1‑140 (MQ=255)          aGGCAATTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTg                                                                                                                       <  1:125923/140‑1 (MQ=255)            gCAATTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGcc                                                                                                                     >  1:639036/1‑140 (MQ=255)            gCAATTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGcc                                                                                                                     >  1:106197/1‑140 (MQ=255)               aTTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCccac                                                                                                                  >  2:1084898/1‑140 (MQ=255)               aTTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCccac                                                                                                                  <  2:437424/140‑1 (MQ=255)               aTTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCccac                                                                                                                  <  2:515279/140‑1 (MQ=255)               aTTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCccac                                                                                                                  <  2:700159/140‑1 (MQ=255)                  ccTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTg                                                                                                                       >  1:172218/1‑132 (MQ=255)                  ccTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTg                                                                                                                       <  2:172218/132‑1 (MQ=255)                     cACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCAGCTGGCGGTGAAGGCGCTCAATAAGGCGCTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTAc                                                                                                            >  1:172348/1‑140 (MQ=255)                         cTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTAccggg                                                                                                        <  1:754104/140‑1 (MQ=255)                           tACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTAccgggcc                                                                                                      >  2:714866/1‑140 (MQ=255)                            acacCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTAccgggccg                                                                                                     >  2:442636/1‑140 (MQ=255)                            acacCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTAccgggccg                                                                                                     >  1:808780/1‑140 (MQ=255)                             cacCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTAccgggccgg                                                                                                    <  2:43095/140‑1 (MQ=255)                             cacCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTAccgggccgg                                                                                                    <  1:868506/140‑1 (MQ=255)                              acCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTAccgggccggg                                                                                                   <  1:386243/140‑1 (MQ=255)                               cccGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACcgggccgggc                                                                                                  <  2:542309/140‑1 (MQ=255)                                  gccgGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGa                                                                                               >  2:1131824/1‑140 (MQ=255)                                  gccgGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGa                                                                                               <  2:399555/140‑1 (MQ=255)                                  gccgGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGa                                                                                               <  2:695260/140‑1 (MQ=255)                                  gccgGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGa                                                                                               >  2:822442/1‑140 (MQ=255)                                  gccgGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGa                                                                                               >  2:702344/1‑140 (MQ=255)                                  gccgGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGa                                                                                               >  1:1386197/1‑140 (MQ=255)                                  gccgGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGa                                                                                               <  2:1072237/140‑1 (MQ=255)                                    cgGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGAtt                                                                                             >  1:753535/1‑140 (MQ=255)                                     ggggAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATta                                                                                            <  2:106197/140‑1 (MQ=255)                                      gggAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATtat                                                                                           <  1:700577/140‑1 (MQ=255)                                       ggAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGAtt                                                                                             >  1:710557/1‑137 (MQ=255)                                       ggAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGAtt                                                                                             <  2:710557/137‑1 (MQ=255)                                         aGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATAtt                                                                                        <  2:639036/140‑1 (MQ=255)                                            aaaGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATtcat                                                                                     <  1:1131824/140‑1 (MQ=255)                                                   aGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTg                                                                              <  1:870082/140‑1 (MQ=255)                                                         tGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCAGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGAtt                                                                        >  1:1293283/1‑140 (MQ=255)                                                          gCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGAttt                                                                       >  2:429935/1‑140 (MQ=255)                                                               tCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCAGCTTGCCGATTTACTgg                                                                  >  2:1254579/1‑140 (MQ=255)                                                                          tGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGaagcaagc                                                       <  1:1254579/140‑1 (MQ=255)                                                                            aaGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCgg                                                     <  2:1056048/140‑1 (MQ=255)                                                                             aGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGa                                                    >  1:363758/1‑140 (MQ=255)                                                                              ggCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGaa                                                   <  2:1235450/140‑1 (MQ=255)                                                                                    cAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTc                                             <  2:253443/140‑1 (MQ=255)                                                                                         aGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCg                                        <  2:363758/140‑1 (MQ=255)                                                                                          ggCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGa                                       >  1:779109/1‑140 (MQ=255)                                                                                              ttGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGc                                   <  2:753535/140‑1 (MQ=255)                                                                                               tGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGcc                                  <  1:822442/140‑1 (MQ=255)                                                                                               tGCTGGCCCATTTTTACGCCGTAGCGAACAGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGcc                                  >  1:961150/1‑140 (MQ=255)                                                                                                gCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCa                                 <  1:429935/140‑1 (MQ=255)                                                                                                gCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCa                                 <  2:1382432/140‑1 (MQ=255)                                                                                                gCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCAGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCa                                 <  2:1293283/140‑1 (MQ=255)                                                                                                 cTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAg                                >  2:96545/1‑140 (MQ=255)                                                                                                           tttACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGat                      >  2:758118/1‑140 (MQ=255)                                                                                                             tACGCCGTAGCGAACTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGatat                    <  2:779109/140‑1 (MQ=255)                                                                                                                          aCTGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCg       <  1:758118/140‑1 (MQ=255)                                                                                                                            tGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGaa     <  1:342776/140‑1 (MQ=255)                                                                                                                            tGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGaa     <  2:733322/140‑1 (MQ=255)                                                                                                                            tGGGATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGaa     <  2:808780/140‑1 (MQ=255)                                                                                                                              ggATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGaa     >  2:532451/1‑138 (MQ=255)                                                                                                                              ggATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGaa     <  1:532451/138‑1 (MQ=255)                                                                                                                               gATATCCCCGATGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTg  <  2:172348/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                 CCTGAACTCAGGCAATTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGGCGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCCGACGGTTTTCTCTGCCCACCAGTACCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTG  >  NC_000913/842413‑842678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑