Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	896,954	T→C	L274P (CTA→CCA)	potH →	putrescine ABC transporter membrane subunit PotH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	896,954	0	T	C	100.0%	266.4 / NA	73	L274P (CTA→CCA)	potH	putrescine ABC transporter membrane subunit PotH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/32);  total (41/32)

TGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGAT  >  NC_000913/896826‑897085                                                                                                                                 |                                                                                                                                    tgtgATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGc                                                                                                                                >  2:1304003/1‑134 (MQ=255) tgtgATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGc                                                                                                                                <  1:1304003/134‑1 (MQ=255)  gtgATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGtt                                                                                                                         >  1:1112393/1‑140 (MQ=255)   tgATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGttt                                                                                                                        >  1:482716/1‑140 (MQ=255)   tgATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGttt                                                                                                                        <  1:178157/140‑1 (MQ=255)   tgATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGttt                                                                                                                        <  1:357867/140‑1 (MQ=255)     aTCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGttttt                                                                                                                      >  1:648886/1‑140 (MQ=255)      tCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTc                                                                                                                     >  2:520322/1‑140 (MQ=255)       cGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAATTTTTCa                                                                                                                    <  2:1107013/140‑1 (MQ=255)        gTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCaa                                                                                                                   >  1:1360092/1‑140 (MQ=255)          gCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATa                                                                                                                 >  1:1300163/1‑140 (MQ=255)          gCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCAGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGCGCCATGGCAAGAGTTTTTCAATa                                                                                                                 >  2:541953/1‑140 (MQ=255)                 aCTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGa                                                                                                          <  2:445127/140‑1 (MQ=255)                        gTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCg                                                                                                   <  2:482716/140‑1 (MQ=255)                               tattGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCt                                                                                            >  2:9043/1‑140 (MQ=255)                               tattGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCt                                                                                            <  2:825697/140‑1 (MQ=255)                                  tGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTcgg                                                                                         <  2:662654/140‑1 (MQ=255)                                         tCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGc                                                                                  <  2:373752/140‑1 (MQ=255)                                              gCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGatca                                                                             >  1:982297/1‑140 (MQ=255)                                                      ttATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtg                                                                     >  1:937932/1‑140 (MQ=255)                                                       tATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtgc                                                                    >  1:22726/1‑140 (MQ=255)                                                       tATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtgc                                                                    <  2:23717/140‑1 (MQ=255)                                                        aTCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtgct                                                                   <  1:312651/140‑1 (MQ=255)                                                        aTCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtgct                                                                   <  1:96766/140‑1 (MQ=255)                                                            cGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtgctgctg                                                               >  2:1204122/1‑140 (MQ=255)                                                                  tgGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATg                                                                        <  1:179614/125‑1 (MQ=255)                                                                  tgGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATg                                                                        >  2:179614/1‑125 (MQ=255)                                                                  tgGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAAtt                                                         >  2:851443/1‑140 (MQ=255)                                                                  ggggCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAAtt                                                         <  1:724449/139‑1 (MQ=255)                                                                   gggCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATtg                                                        <  1:851443/140‑1 (MQ=255)                                                                    ggCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtgct                                                                   <  2:295561/128‑1 (MQ=255)                                                                    ggCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTtgct                                                                   >  1:295561/1‑128 (MQ=255)                                                                    ggCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATtgt                                                       <  2:648886/140‑1 (MQ=255)                                                                      cGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGCTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGc                                                     <  2:1305075/140‑1 (MQ=255)                                                                                  cccGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGt                                         >  1:297259/1‑140 (MQ=255)                                                                                   ccGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGtt                                        >  2:366842/1‑140 (MQ=255)                                                                                   ccGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGtt                                        >  2:1328036/1‑140 (MQ=255)                                                                                    cGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTc                                       >  1:706722/1‑140 (MQ=255)                                                                                            cTCGGTGGCCCGGACAGTATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAaca                               >  1:793425/1‑140 (MQ=255)                                                                                            cTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAaca                               >  1:1027287/1‑140 (MQ=255)                                                                                            cTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAaca                               >  2:592448/1‑140 (MQ=255)                                                                                            cTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAaca                               >  1:501781/1‑140 (MQ=255)                                                                                            cTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAaca                               >  2:774208/1‑140 (MQ=255)                                                                                            cTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAaca                               >  2:898928/1‑140 (MQ=255)                                                                                            cTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAaca                               >  2:1383801/1‑140 (MQ=255)                                                                                            cTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAaca                               >  2:946481/1‑140 (MQ=255)                                                                                            cTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAaca                               >  1:915157/1‑140 (MQ=255)                                                                                            cTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAaca                               >  2:951991/1‑140 (MQ=255)                                                                                             tCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAacac                              <  1:1204122/140‑1 (MQ=255)                                                                                                     ccGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCaaaa                      <  2:706722/140‑1 (MQ=255)                                                                                                      cGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAac                                >  2:740/1‑129 (MQ=255)                                                                                                      cGGACAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAac                                <  1:740/129‑1 (MQ=255)                                                                                                          cAGCATCATGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCg                 <  1:592448/140‑1 (MQ=255)                                                                                                             catcatGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTgg              <  1:541953/140‑1 (MQ=255)                                                                                                             catcatGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTgg              <  2:1238809/140‑1 (MQ=255)                                                                                                             catcatGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTgg              <  1:774208/140‑1 (MQ=255)                                                                                                               tcatGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGga            >  2:420917/1‑140 (MQ=255)                                                                                                               tcatGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGga            <  1:814235/140‑1 (MQ=255)                                                                                                                catGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGgag           <  1:9043/140‑1 (MQ=255)                                                                                                                  tGATCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGaa         >  1:185707/1‑140 (MQ=255)                                                                                                                    aTCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAaca       <  1:1328036/140‑1 (MQ=255)                                                                                                                    aTCGGGCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAaca       <  2:1027287/140‑1 (MQ=255)                                                                                                                       gggCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTGATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACgg    >  1:410870/1‑140 (MQ=255)                                                                                                                       gggCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTgg              >  1:2610/1‑130 (MQ=255)                                                                                                                       gggCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTgg              <  2:2610/130‑1 (MQ=255)                                                                                                                       gggCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACgg    <  2:982297/140‑1 (MQ=255)                                                                                                                       gggCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAAAGTGGTTCCCCAAACACCAGCAAAAAAGCGTGGGAGAACACgg    >  2:697367/1‑140 (MQ=255)                                                                                                                       gggCGCGTGCCATGGCAAGAGGTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACgg    >  2:889857/1‑140 (MQ=255)                                                                                                                        ggCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGa   >  1:483918/1‑140 (MQ=255)                                                                                                                        ggCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGa   >  2:405749/1‑140 (MQ=255)                                                                                                                        ggCGCGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGa   >  2:569670/1‑140 (MQ=255)                                                                                                                         gcgcGTGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGAt  >  2:469788/1‑140 (MQ=255)                                                                                                                              tGCCATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTgg              <  2:367948/123‑1 (MQ=255)                                                                                                                                 |                                                                                                                                    TGTGATCGTGCCGCTGACTAAAGGTGGGATTATTGCCGGATCGATGCTGGTGTTTATCCCGGCTGTGGGCGAGTTTGTGATCCCGGAACTGCTCGGTGGCCCGGACAGCATCATGATCGGGCGCGTGCTATGGCAAGAGTTTTTCAATAACCGCGACTGGCCGGTGGCCTCGGCGGTAGCGATCATCATGTTGCTGCTGCTAATTGTGCCGATAATGTGGTTCCACAAACACCAGCAAAAAAGCGTGGGAGAACACGGAT  >  NC_000913/896826‑897085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑