Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	911,302	T→C	T283T (ACA→ACG)	hcr ←	NADH oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	911,302	0	T	C	100.0%	198.4 / NA	55	T283T (ACA→ACG)	hcr	NADH oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/22);  total (33/22)

GGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTT  >  NC_000913/911169‑911433                                                                                                                                      |                                                                                                                                    gggCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTa                                                                                                                               >  1:275618/1‑140 (MQ=255)  ggCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTAc                                                                                                                              >  2:1047550/1‑140 (MQ=255)  ggCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTAc                                                                                                                              >  2:841378/1‑140 (MQ=255)            gATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCa                                                                                                                    <  2:1372524/140‑1 (MQ=255)             aTTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCaa                                                                                                                   >  1:681417/1‑140 (MQ=255)                   cGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACAccc                                                                                                             >  1:713078/1‑140 (MQ=255)                    gagaACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCg                                                                                                            >  1:469786/1‑140 (MQ=255)                        aCCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACg                                                                                                        <  2:497294/140‑1 (MQ=255)                          cAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACggc                                                                                                      <  2:469786/140‑1 (MQ=255)                            aaTCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACggcag                                                                                                    <  2:713078/140‑1 (MQ=255)                                  ccTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCg                                                                                              >  2:631867/1‑140 (MQ=255)                                       ggATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAAc                                                                                         <  2:450985/140‑1 (MQ=255)                                              aGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACg                                                                                  >  1:508960/1‑140 (MQ=255)                                                   cAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTAtt                                                                             >  1:1251728/1‑140 (MQ=255)                                                      gCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGTAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCt                                                                          >  2:1295655/1‑140 (MQ=255)                                                       ccAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCtt                                                                         >  2:511549/1‑140 (MQ=255)                                                              gTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAgcg                                                                  <  2:275618/140‑1 (MQ=255)                                                                aaCCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCGCTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGcc                                                                >  2:1230773/1‑140 (MQ=255)                                                                aaCCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCa                                                                     <  1:309496/135‑1 (MQ=255)                                                                aaCCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCa                                                                     >  2:309496/1‑135 (MQ=255)                                                                aaCCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGcc                                                                >  1:849662/1‑140 (MQ=255)                                                                     tCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAg                                                           >  2:273281/1‑140 (MQ=255)                                                                       aGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTCCAGCAGCCGCAAACACCCACACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAgcgc                                                                 <  2:1246815/132‑1 (MQ=255)                                                                       aGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAgcgc                                                                 >  1:1246815/1‑132 (MQ=255)                                                                          gATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCg                                                      <  2:426908/140‑1 (MQ=255)                                                                          gATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCg                                                      <  2:849662/140‑1 (MQ=255)                                                                                      ggTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCg                                          >  1:308365/1‑140 (MQ=255)                                                                                       gTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCgg                                         <  1:631867/140‑1 (MQ=255)                                                                                       gTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCgg                                         >  1:1179547/1‑140 (MQ=255)                                                                                        tCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCggg                                        <  1:1230773/140‑1 (MQ=255)                                                                                        tCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCggg                                        <  1:830441/140‑1 (MQ=255)                                                                                           gCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCTCTTGCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCg                                     <  2:845668/140‑1 (MQ=255)                                                                                                 ttGTGCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAATAGCGTGGTGCCAACCGGGGCGTAAAAt                               >  2:826858/1‑140 (MQ=255)                                                                                                  tgtgCTGCTCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAAtt                              >  2:1089986/1‑140 (MQ=255)                                                                                                         ctCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGc                       >  2:343729/1‑140 (MQ=255)                                                                                                         ctCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGc                       <  1:1143113/140‑1 (MQ=255)                                                                                                         ctCACCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGc                       <  1:964528/140‑1 (MQ=255)                                                                                                            aCCGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCgg                    >  2:774472/1‑140 (MQ=255)                                                                                                              cGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGt                         >  2:343056/1‑133 (MQ=255)                                                                                                              cGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGt                         <  1:343056/133‑1 (MQ=255)                                                                                                              cGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGc                       >  1:1078914/1‑135 (MQ=255)                                                                                                              cGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGc                       <  2:1078914/135‑1 (MQ=255)                                                                                                              cGTATATTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGtt                  >  2:29073/1‑140 (MQ=255)                                                                                                               gTATACTCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTg                 <  2:1237881/140‑1 (MQ=255)                                                                                                                    ttCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGtt            <  1:273281/140‑1 (MQ=255)                                                                                                                    ttCACCGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGtt            <  2:586096/140‑1 (MQ=255)                                                                                                                        ccGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGt             <  2:1182589/135‑1 (MQ=255)                                                                                                                        ccGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGt             >  1:1182589/1‑135 (MQ=255)                                                                                                                        ccGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGt        >  1:650888/1‑140 (MQ=255)                                                                                                                        ccGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGt        <  2:508960/140‑1 (MQ=255)                                                                                                                        ccGGAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGt        >  1:193609/1‑140 (MQ=255)                                                                                                                          ggAAACCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGa      >  1:752567/1‑140 (MQ=255)                                                                                                                            aaaCCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAAt    >  2:527587/1‑140 (MQ=255)                                                                                                                            aaaCCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAAt    >  1:759755/1‑140 (MQ=255)                                                                                                                              aCCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAAttt  >  1:1257972/1‑140 (MQ=255)                                                                                                                              aCCACTTTCGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAAttt  >  1:983174/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                    GGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCATTGTGCTGCTCACCGTATATTCACCGGAAACCACTTTTGTCTTACAGCAGCCGCAAACACCCGCACGGCAGGCAGCGACAACCGGAACGTTATTGCTTTCCAGCGCCTCCAGTAGCGTGGTGCCAACCGGGGCGTAAAATTCTCGTGCCGGTTGCAGTTTGGTGAATTT  >  NC_000913/911169‑911433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑