Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	986,142	A→G	L281L (TTA→CTA)	ompF ←	outer membrane porin F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	986,142	0	A	G	98.0%	352.1 / ‑2.5	100	L281L (TTA→CTA)	ompF	outer membrane porin F
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (52/46);  total (53/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

TGAAGTAGTAGGTTGCGCCCACTTCAAAGTAGTTCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAACAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTAGCCCA  >  NC_000913/986007‑986277                                                                                                                                        |                                                                                                                                        tgAAGTAGTAGGTTGCGCCCACTTCAAAGTAGTTCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGa                                                                                                                                     <  2:516513/140‑1 (MQ=255)                cgcCCACTTCAAAGTAGTTCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTttg                                                                                                                      >  1:1343683/1‑140 (MQ=255)                cgcCCACTTCAAAGTAGTTCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTttg                                                                                                                      >  2:1070541/1‑140 (MQ=255)                cgcCCACTTCAAAGTAGTTCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAACAGAACGTCTTGCGTTttg                                                                                                                      >  1:793509/1‑140 (MQ=255)                  cccACTTCAAAGTAGTTCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTttgtt                                                                                                                    >  2:485766/1‑140 (MQ=255)                       ttCAAAGTAGTTCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGa                                                                                                               <  1:829463/140‑1 (MQ=255)                       ttCAAAGTAGTTCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGa                                                                                                               <  1:572185/140‑1 (MQ=255)                       ttCAAAGTAGTTCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGa                                                                                                               <  2:843425/140‑1 (MQ=255)                       ttCAAAGTAGTTCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGa                                                                                                               <  2:1342585/140‑1 (MQ=255)                        tCAAAGTAGTTCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGaa                                                                                                              <  2:422567/140‑1 (MQ=255)                             gtagtTCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGc                                                                                                         >  1:82485/1‑140 (MQ=255)                                gtTCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTgg                                                                                                      >  1:1363160/1‑140 (MQ=255)                                 ttCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGc                                                                                                                 >  2:1362774/1‑128 (MQ=255)                                 ttCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGc                                                                                                                 <  1:1362774/128‑1 (MQ=255)                                 ttCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGgt                                                                                                     <  1:1012840/140‑1 (MQ=255)                                 ttCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGgt                                                                                                     >  2:29172/1‑140 (MQ=255)                                 ttCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGgt                                                                                                     >  1:690985/1‑140 (MQ=255)                                 ttCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGgt                                                                                                     >  2:459833/1‑140 (MQ=255)                                 ttCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGgt                                                                                                     >  2:969298/1‑140 (MQ=255)                                 ttCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGgt                                                                                                     >  1:1242638/1‑140 (MQ=255)                                       aGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGt                                                                                               <  1:1226470/140‑1 (MQ=255)                                       aGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGt                                                                                               <  1:1013176/140‑1 (MQ=255)                                       aGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGt                                                                                               <  1:1179148/140‑1 (MQ=255)                                       aGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAACAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGt                                                                                               <  2:793509/140‑1 (MQ=255)                                        gATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTa                                                                                              >  2:1395811/1‑140 (MQ=255)                                              cATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATtta                                                                                        >  2:742014/1‑140 (MQ=255)                                              cATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATtta                                                                                        >  2:852414/1‑140 (MQ=255)                                              cATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATtta                                                                                        >  1:119695/1‑140 (MQ=255)                                              cATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATtta                                                                                        >  2:1270689/1‑140 (MQ=255)                                                   ccGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGt                                                                                   >  1:172224/1‑140 (MQ=255)                                                   ccGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGt                                                                                   >  2:342198/1‑140 (MQ=255)                                                   ccGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGt                                                                                   >  2:437050/1‑140 (MQ=255)                                                   ccGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGt                                                                                   >  2:1327213/1‑140 (MQ=255)                                                    cGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTg                                                                                  >  2:12814/1‑140 (MQ=255)                                                     gATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGa                                                                                 <  2:930408/140‑1 (MQ=255)                                                      aTACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGAt                                                                                <  1:437050/140‑1 (MQ=255)                                                      aTACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGAt                                                                                <  2:1363160/140‑1 (MQ=255)                                                         ccTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCgg                                                                             >  1:125101/1‑140 (MQ=255)                                                            tCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGAt                                                                                >  1:180883/1‑134 (MQ=255)                                                            tCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGAt                                                                                <  2:180883/134‑1 (MQ=255)                                                            tCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGt                                                                          >  2:357183/1‑140 (MQ=255)                                                            tCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGt                                                                          <  1:1007393/140‑1 (MQ=255)                                                            tCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGt                                                                          >  1:782855/1‑140 (MQ=255)                                                            tCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGt                                                                          >  1:625901/1‑140 (MQ=255)                                                            tCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGt                                                                          <  1:376872/140‑1 (MQ=255)                                                              tACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAg                                                                        >  1:1037970/1‑140 (MQ=255)                                                                cGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCg                                                                      >  1:768562/1‑140 (MQ=255)                                                                    tttCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTAc                                                                  >  2:760808/1‑140 (MQ=255)                                                                     ttCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACg                                                                 <  2:118514/140‑1 (MQ=255)                                                                      tCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCTGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACgg                                                                >  1:1154109/1‑140 (MQ=255)                                                                      tCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACgg                                                                >  2:554723/1‑140 (MQ=255)                                                                      tCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACgg                                                                >  2:412240/1‑140 (MQ=255)                                                                      tCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACgg                                                                >  1:453785/1‑140 (MQ=255)                                                                       cGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACggg                                                               <  1:1070541/140‑1 (MQ=255)                                                                       cGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACggg                                                               >  1:1203327/1‑140 (MQ=255)                                                                       cGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACggg                                                               >  2:1397354/1‑140 (MQ=255)                                                                           ttAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTc                                                           >  1:426522/1‑140 (MQ=255)                                                                                   ggTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGt                                                   >  2:165180/1‑140 (MQ=255)                                                                                    gtgtAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGtt                                                  <  2:690985/140‑1 (MQ=255)                                                                                      gtAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCg                                                <  1:12814/140‑1 (MQ=255)                                                                                         aGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTg                                             <  1:554723/140‑1 (MQ=255)                                                                                         aGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTg                                             >  1:405408/1‑140 (MQ=255)                                                                                          gCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGc                                            <  1:1270689/140‑1 (MQ=255)                                                                                          gCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGc                                            <  1:1327213/140‑1 (MQ=255)                                                                                          gCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGc                                            <  2:238668/140‑1 (MQ=255)                                                                                          gCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGc                                            <  1:342198/140‑1 (MQ=255)                                                                                              tGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAgg                                        <  1:412240/140‑1 (MQ=255)                                                                                              tGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAgg                                        >  2:867355/1‑140 (MQ=255)                                                                                               ggacggacGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGt                                       >  2:812397/1‑140 (MQ=255)                                                                                               ggacggacGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGt                                       >  2:714997/1‑140 (MQ=255)                                                                                               ggacggacGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGt                                       >  1:255322/1‑140 (MQ=255)                                                                                               ggacggacGCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGt                                       >  2:455152/1‑140 (MQ=255)                                                                                                 acggacgCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAg                                     <  1:969298/140‑1 (MQ=255)                                                                                                     acgCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGAtgt                                 >  1:552347/1‑140 (MQ=255)                                                                                                     acgCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGAtgt                                 >  1:999956/1‑140 (MQ=255)                                                                                                      cgCAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGAtgtt                                >  1:598997/1‑140 (MQ=255)                                                                                                        cAGACCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGAtgttgt                              <  2:453785/140‑1 (MQ=255)                                                                                                           aCCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTcg                           <  1:1395811/140‑1 (MQ=255)                                                                                                           aCCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTcg                           <  1:357183/140‑1 (MQ=255)                                                                                                           aCCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTcg                           <  2:782855/140‑1 (MQ=255)                                                                                                           aCCGAAATCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTcg                           <  1:485766/140‑1 (MQ=255)                                                                                                                aaTCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGcgtcg                      <  2:1037970/140‑1 (MQ=255)                                                                                                                aaTCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGcgtcg                      <  2:255322/140‑1 (MQ=255)                                                                                                                  tCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTa                    <  2:768562/140‑1 (MQ=255)                                                                                                                  tCGAACTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTa                    <  2:564359/140‑1 (MQ=255)                                                                                                                       cTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCa               <  1:812397/140‑1 (MQ=255)                                                                                                                       cTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCa               <  1:29172/140‑1 (MQ=255)                                                                                                                       cTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCa               >  2:888923/1‑140 (MQ=255)                                                                                                                       cTGGTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCa               <  1:714997/140‑1 (MQ=255)                                                                                                                          gTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGAc            <  2:125101/140‑1 (MQ=255)                                                                                                                          gTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGAc            <  1:66121/140‑1 (MQ=255)                                                                                                                          gTATTGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGAc            >  2:880486/1‑140 (MQ=255)                                                                                                                             ttGCGCAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAg         <  1:459833/140‑1 (MQ=255)                                                                                                                               gtgcAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTa       >  2:1173989/3‑140 (MQ=255)                                                                                                                               gcgcAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTa       <  2:215168/140‑1 (MQ=255)                                                                                                                                cgcAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTAg      >  2:792106/1‑140 (MQ=255)                                                                                                                                  cAACTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTAGcc    >  1:562463/1‑140 (MQ=255)                                                                                                                                    aCTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTAGCCCa  <  2:172224/140‑1 (MQ=255)                                                                                                                                    aCTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTAGCCCa  >  2:724374/1‑140 (MQ=255)                                                                                                                                    aCTAGCAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTAGCCCa  <  2:999956/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TGAAGTAGTAGGTTGCGCCCACTTCAAAGTAGTTCACCAGATCAACATCACCGATACCTTCTACGTCTTTCGCTTTAGATTTGGTGTAAGCGATGGACGGACGCAGACCGAAATCGAACTGGTATTGCGCAACTAACAGAACGTCTTGCGTTTTGTTGGCGAAGCCGCTGGTGTTTGTAAATTTATTAGTGATCGGCGTAGCGTTACGGGTTTCACCGTAGTTCGCTGCCAGGTAGATGTTGTTCGCGTCGTACTTCAGACCAGTAGCCCA  >  NC_000913/986007‑986277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑