Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,136,183	A→G	Y207C (TAC→TGC)	flgH →	flagellar L‑ring protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,136,183	0	A	G	100.0%	337.8 / NA	92	Y207C (TAC→TGC)	flgH	flagellar L‑ring protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/48);  total (44/48)

CATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTACTGGTCACGAC  >  NC_000913/1136047‑1136317                                                                                                                                         |                                                                                                                                       cATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGt                                                                                                                                     >  1:699574/1‑140 (MQ=255)     tggtggGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGc                                                                                                                                 <  1:897550/140‑1 (MQ=255)     tggtggGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGc                                                                                                                                 <  2:1391830/140‑1 (MQ=255)      ggtgggtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCa                                                                                                                                <  2:511838/140‑1 (MQ=255)      ggtgggtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCa                                                                                                                                <  2:1261652/140‑1 (MQ=255)      ggtgggtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCa                                                                                                                                <  1:826093/140‑1 (MQ=255)       gtgggtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCaa                                                                                                                               >  2:842173/1‑140 (MQ=255)        tgggtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAAt                                                                                                                              >  2:1307270/1‑140 (MQ=255)         gggtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATg                                                                                                                             <  1:126807/140‑1 (MQ=255)          ggtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATgg                                                                                                                            <  2:701529/140‑1 (MQ=255)          ggtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATgg                                                                                                                            <  2:259333/140‑1 (MQ=255)           gtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGc                                                                                                                           <  1:1384518/140‑1 (MQ=255)           gtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTCGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATg                                                                                                                             >  1:218197/1‑138 (MQ=255)           gtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTCGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATg                                                                                                                             <  2:218197/138‑1 (MQ=255)                aaaCAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACAt                                                                                                                      >  1:874814/1‑140 (MQ=255)                     gATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACg                                                                                                                 <  2:592856/140‑1 (MQ=255)                     gATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACg                                                                                                                 <  1:1352787/140‑1 (MQ=255)                     gATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACg                                                                                                                 <  2:1209228/140‑1 (MQ=255)                      aTTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGa                                                                                                                >  1:935104/1‑140 (MQ=255)                      aTTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGa                                                                                                                >  2:458582/1‑140 (MQ=255)                                     ggTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATggg                                                                                                 >  1:889237/1‑140 (MQ=255)                                     ggTACAGAATTTATTCGCTTCTCTGGAGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTATGCAATGGCTACATTAACGAAGCGCAAAATATggg                                                                                                 <  2:1237990/140‑1 (MQ=255)                                      gTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGc                                                                                                >  1:799165/1‑140 (MQ=255)                                       tACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCt                                                                                               <  2:646097/140‑1 (MQ=255)                                          cGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGt                                                                                            >  1:538246/1‑140 (MQ=255)                                          cGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGt                                                                                            >  2:439428/1‑140 (MQ=255)                                          cGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGt                                                                                            >  2:1139183/1‑140 (MQ=255)                                           gAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGtt                                                                                           >  1:900031/1‑140 (MQ=255)                                            aaTTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTg                                                                                          >  2:702999/1‑140 (MQ=255)                                             aTTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGc                                                                                         <  1:2938/140‑1 (MQ=255)                                             aTTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGc                                                                                         <  1:787530/140‑1 (MQ=255)                                                   tCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGtt                                                                                   <  1:974931/140‑1 (MQ=255)                                                    cGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGttt                                                                                  <  1:702999/140‑1 (MQ=255)                                                    cGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGttt                                                                                  <  2:699574/140‑1 (MQ=255)                                                    cGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGttt                                                                                  <  2:538246/140‑1 (MQ=255)                                                    cGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGttt                                                                                  <  2:799165/140‑1 (MQ=255)                                                      cTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTttct                                                                                >  1:57775/1‑140 (MQ=255)                                                           ctGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCtt                                                                           <  2:1342013/140‑1 (MQ=255)                                                                    ttAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCg                                                                  <  1:224003/140‑1 (MQ=255)                                                                    ttAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCg                                                                  >  2:891300/1‑140 (MQ=255)                                                                    ttAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCg                                                                  <  1:1139191/140‑1 (MQ=255)                                                                     tAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGc                                                                 <  2:889237/140‑1 (MQ=255)                                                                     tAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGc                                                                 <  2:900031/140‑1 (MQ=255)                                                                     tAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGc                                                                 <  1:439428/140‑1 (MQ=255)                                                                           aCGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGt                                                           <  2:935104/140‑1 (MQ=255)                                                                             gCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCtt                                                                           <  1:272446/122‑1 (MQ=255)                                                                             gCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCtt                                                                           >  2:272446/1‑122 (MQ=255)                                                                             gCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTaa                                                         >  2:744770/1‑140 (MQ=255)                                                                             gCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTaa                                                         >  2:1298779/1‑140 (MQ=255)                                                                             gCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTaa                                                         >  2:1064545/1‑140 (MQ=255)                                                                             gCACTATCAGCCGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCTCCAATGTaa                                                         >  2:1356406/1‑140 (MQ=255)                                                                              cACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGTCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAg                                                        >  1:571257/1‑140 (MQ=255)                                                                              cACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGc                                                                 >  1:404154/1‑131 (MQ=255)                                                                              cACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGc                                                                 <  2:404154/131‑1 (MQ=255)                                                                              cACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAg                                                        >  2:55586/1‑140 (MQ=255)                                                                              cACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAg                                                        <  1:707971/140‑1 (MQ=255)                                                                               aCTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGt                                                       <  2:57775/140‑1 (MQ=255)                                                                               aCTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGt                                                       <  1:55586/140‑1 (MQ=255)                                                                                  aTCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAg                                                    >  2:222163/1‑140 (MQ=255)                                                                                  aTCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAg                                                    >  2:290012/1‑140 (MQ=255)                                                                                   tCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAgg                                                   <  1:891300/140‑1 (MQ=255)                                                                                       cGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCtgt                                               >  2:155740/1‑140 (MQ=255)                                                                                         gcagcaATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTgtgg                                             >  1:1386315/1‑140 (MQ=255)                                                                                           agcaATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTgtggtg                                           >  1:720383/1‑140 (MQ=255)                                                                                           agcaATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTgtggtg                                           >  2:1277181/1‑140 (MQ=255)                                                                                                    gtaccgtCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAAttt                                  >  2:928244/1‑140 (MQ=255)                                                                                                    gtaccgtCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAAttt                                  >  2:639454/1‑140 (MQ=255)                                                                                                      accgtCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTtct                                >  2:108416/1‑140 (MQ=255)                                                                                                       ccgtCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTtctc                               <  1:809743/140‑1 (MQ=255)                                                                                                         gtCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTtctctc                             <  1:290012/140‑1 (MQ=255)                                                                                                          tCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTtctct                              <  2:714454/138‑1 (MQ=255)                                                                                                          tCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTtctct                              >  1:714454/1‑138 (MQ=255)                                                                                                          tCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTctctct                            >  1:1192130/1‑140 (MQ=255)                                                                                                               tCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCAtt                       >  2:548575/1‑140 (MQ=255)                                                                                                               tCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCAtt                       <  1:928244/140‑1 (MQ=255)                                                                                                               tCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCAtt                       <  1:1277181/140‑1 (MQ=255)                                                                                                               tCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCAtt                       <  1:1284300/140‑1 (MQ=255)                                                                                                               tCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCAtt                       <  2:720383/140‑1 (MQ=255)                                                                                                               tCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCAtt                       <  1:639454/140‑1 (MQ=255)                                                                                                                 aGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGCGAGGCTGTGGTGATTAAATTTCTCTCTGCATTaa                     <  1:1298779/140‑1 (MQ=255)                                                                                                                    tGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAAttc                  >  2:834480/1‑140 (MQ=255)                                                                                                                    tGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAAttc                  >  1:575316/1‑140 (MQ=255)                                                                                                                      gCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTtctct                              >  2:158281/1‑126 (MQ=255)                                                                                                                      gCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTtctct                              <  1:158281/126‑1 (MQ=255)                                                                                                                      gCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAAttctt                >  2:763678/1‑140 (MQ=255)                                                                                                                         gATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCAtt                       >  1:714626/1‑130 (MQ=255)                                                                                                                         gATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCAtt                       <  2:714626/130‑1 (MQ=255)                                                                                                                         gATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTa             <  1:460775/140‑1 (MQ=255)                                                                                                                             cgcgcATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAAt                    >  2:731576/1‑129 (MQ=255)                                                                                                                             cgcgcATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAAt                    <  1:731576/129‑1 (MQ=255)                                                                                                                             cgcgcATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTACTgg         >  1:312287/1‑140 (MQ=255)                                                                                                                             cgcgcATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTACTgg         >  1:911740/1‑140 (MQ=255)                                                                                                                                    tGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTACTGGTCacgac  >  1:55835/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTACTGGTCACGAC  >  NC_000913/1136047‑1136317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑