Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	177,839	C→T	S208S (TCG→TCA)	btuF ←	vitamin B12 ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	177,839	0	C	T	100.0%	291.5 / NA	84	S208S (TCG→TCA)	btuF	vitamin B12 ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/38);  total (46/38)

CGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTA  >  NC_000913/177703‑177971                                                                                                                                         |                                                                                                                                     cGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAg                                                                                                                                   <  1:44806/140‑1 (MQ=255) cGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAg                                                                                                                                   <  1:1202329/140‑1 (MQ=255) cGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAg                                                                                                                                   <  2:1049531/140‑1 (MQ=255) cGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAg                                                                                                                                   <  2:611731/140‑1 (MQ=255)  gAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGc                                                                                                                                  <  1:320106/140‑1 (MQ=255)  gAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGc                                                                                                                                  <  2:1320102/140‑1 (MQ=255)  gAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGc                                                                                                                                  <  1:614530/140‑1 (MQ=255)   aGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCg                                                                                                                                 >  1:1215818/1‑140 (MQ=255)   aGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCg                                                                                                                                 >  2:1286794/1‑140 (MQ=255)      ataataCGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGc                                                                                                                              >  2:1365377/1‑140 (MQ=255)               gggCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGACTTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTg                                                                                                                     >  1:955632/1‑140 (MQ=255)               gggCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTg                                                                                                                     >  1:1290849/1‑140 (MQ=255)               gggCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTg                                                                                                                     >  2:199920/1‑140 (MQ=255)                ggCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGt                                                                                                                    >  1:464014/1‑140 (MQ=255)                 gCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGtt                                                                                                                   <  2:550588/140‑1 (MQ=255)                  cTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTc                                                                                                                  >  2:260037/1‑140 (MQ=255)                    tGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTcgc                                                                                                                >  2:988556/1‑140 (MQ=255)                    tGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTcgc                                                                                                                >  1:1168913/1‑140 (MQ=255)                      cACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCgg                                                                                                              >  2:179174/1‑140 (MQ=255)                      cACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCgg                                                                                                              >  1:940866/1‑140 (MQ=255)                       aCGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGc                                                                                                             <  1:1078905/140‑1 (MQ=255)                           tCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAAc                                                                                                         >  2:1139334/1‑140 (MQ=255)                           tCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAAc                                                                                                         >  2:1396437/1‑140 (MQ=255)                             aaaCCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACtt                                                                                                       <  1:1365377/140‑1 (MQ=255)                              aaCCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTg                                                                                                      <  2:940866/140‑1 (MQ=255)                                    tCACTCGTGATAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCt                                                                                                            >  2:853996/1‑128 (MQ=255)                                    tCACTCGTGATAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCt                                                                                                            <  1:853996/128‑1 (MQ=255)                                    tCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGATCGTGCTAACACCTGTTCGCGGCt                                                                                                            >  2:965803/1‑128 (MQ=255)                                    tCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCt                                                                                                            <  1:965803/128‑1 (MQ=255)                                    tCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCa                                                                                                >  1:1320608/1‑140 (MQ=255)                                      aCTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGc                                                                                                     <  2:1218050/133‑1 (MQ=255)                                      aCTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGc                                                                                                     >  1:1218050/1‑133 (MQ=255)                                         cGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGaa                                                                                           >  2:307973/1‑140 (MQ=255)                                             agagGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGATAACTTGCGGCCAGGGAACCCg                                                                                       >  1:987792/1‑140 (MQ=255)                                              gagGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGaa                                                                                           >  1:539682/1‑135 (MQ=255)                                              gagGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGaa                                                                                           <  2:539682/135‑1 (MQ=255)                                              gagGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCgg                                                                                      <  2:1215818/140‑1 (MQ=255)                                              gagGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCgg                                                                                      <  2:1290849/140‑1 (MQ=255)                                               agGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGc                                                                                     <  1:1139334/140‑1 (MQ=255)                                               agGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGc                                                                                     <  2:1308925/140‑1 (MQ=255)                                                ggAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCt                                                                                    <  2:464014/140‑1 (MQ=255)                                                ggAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCt                                                                                    <  2:316810/140‑1 (MQ=255)                                                ggAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCt                                                                                    <  1:199920/140‑1 (MQ=255)                                                ggAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCt                                                                                    <  2:1364478/140‑1 (MQ=255)                                                     aaCGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCtt                                                                               <  2:540995/140‑1 (MQ=255)                                                         ggAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGc                                                                                     >  1:649800/1‑130 (MQ=255)                                                         ggAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGc                                                                                     <  2:649800/130‑1 (MQ=255)                                                         ggAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTaaa                                                                           >  1:1299795/1‑140 (MQ=255)                                                          gAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAg                                                                          >  2:969092/1‑140 (MQ=255)                                                           aaTTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGa                                                                         >  1:244387/1‑140 (MQ=255)                                                           aaTTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGa                                                                         >  2:55995/1‑140 (MQ=255)                                                            aTTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGAt                                                                        >  1:636290/1‑140 (MQ=255)                                                            aTTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGAt                                                                        >  2:673313/1‑140 (MQ=255)                                                                  aGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTtc                                                                  >  2:497243/1‑140 (MQ=255)                                                                       ttCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGc                                                             >  2:763379/1‑140 (MQ=255)                                                                              cAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAAc                                                      <  2:987792/140‑1 (MQ=255)                                                                                 tATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTc                                                   >  1:1334048/1‑140 (MQ=255)                                                                                 tATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTc                                                   <  2:1320608/140‑1 (MQ=255)                                                                                 tATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCAGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTc                                                   >  1:1379683/1‑140 (MQ=255)                                                                                     gTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGt                                               <  1:589885/140‑1 (MQ=255)                                                                                      tttGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTa                                              >  2:560287/1‑140 (MQ=255)                                                                                      tttGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTa                                              >  1:138749/1‑140 (MQ=255)                                                                                        tGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTAcc                                            <  2:138749/140‑1 (MQ=255)                                                                                        tGATTTTAGGAATTTGGCCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGACCCGGGCTGTCTTAAAAGATTTTTTCTCCGCCACAAACTTCGAGTAcc                                            <  2:287710/140‑1 (MQ=255)                                                                                           ttttAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTgg                                         >  1:1136051/1‑140 (MQ=255)                                                                                              tAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTc                                      >  1:1247599/1‑140 (MQ=255)                                                                                               aGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCt                                     <  1:988556/140‑1 (MQ=255)                                                                                                ggAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTg                                    >  2:847337/1‑140 (MQ=255)                                                                                                 gAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGa                                   >  2:732506/1‑140 (MQ=255)                                                                                                   aTTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAAt                                 <  2:1136051/140‑1 (MQ=255)                                                                                                      tGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGa                              <  2:636290/140‑1 (MQ=255)                                                                                                      gggtccggcccGCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTTTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTTTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGa                              <  2:357333/139‑1 (MQ=255)                                                                                                             gtccgCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCtttt                       >  1:665582/1‑140 (MQ=255)                                                                                                              tccgCCTGTAATGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTc                      >  2:608045/1‑140 (MQ=255)                                                                                                                        aTGACAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTaaa            <  1:55995/140‑1 (MQ=255)                                                                                                                           aCAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTc                      <  2:565373/127‑1 (MQ=255)                                                                                                                           aCAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTc                      >  1:565373/1‑127 (MQ=255)                                                                                                                           aCAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATgg         <  1:1286794/140‑1 (MQ=255)                                                                                                                            cAATCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGc        >  1:134949/1‑140 (MQ=255)                                                                                                                             aaTCGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCg       <  2:134949/140‑1 (MQ=255)                                                                                                                                cGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGAt    >  1:529563/1‑140 (MQ=255)                                                                                                                                cGCCTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGAt    <  1:307973/140‑1 (MQ=255)                                                                                                                                  ccTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTa  >  1:407108/1‑140 (MQ=255)                                                                                                                                  ccTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTa  >  2:1281842/1‑140 (MQ=255)                                                                                                                                  ccTGTGGTGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTa  >  2:168121/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     CGAGGATAATACGTGGGCTTGCACGTTCAAACCAGTCACTCGTGAGAGGAATAACGGGAATTTTGAGCTGTTCACCCCAGTATTGTTTGATTTTAGGAATTTGGTCCGGTCCGCCTGTAATGACAATCGCCTGTGGCGAGCGTGCTAACACCTGTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTA  >  NC_000913/177703‑177971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑