Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,318,251	A→G	R55R (CGT→CGC)	trpB ←	tryptophan synthase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,318,251	0	A	G	100.0%	142.9 / NA	40	R55R (CGT→CGC)	trpB	tryptophan synthase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/16);  total (24/16)

ATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTTGTGGCACGTACATG  >  NC_000913/1318114‑1318374                                                                                                                                          |                                                                                                                            aTCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCg                                                                                                                           >  1:556243/1‑140 (MQ=255)         cGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCAt                                                                                                                   >  2:456609/1‑140 (MQ=255)                     cTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTcagca                                                                                                       <  1:1073711/140‑1 (MQ=255)                     cTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTcagca                                                                                                       <  1:1148481/140‑1 (MQ=255)                      tGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTcagcag                                                                                                      >  1:317550/1‑140 (MQ=255)                       gCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAgg                                                                                                     <  2:395282/140‑1 (MQ=255)                          ccAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCg                                                                                                  <  2:805706/140‑1 (MQ=255)                                         ttttATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGa                                                                                   >  2:607405/1‑140 (MQ=255)                                             aTGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAAtt                                                                               >  1:1200982/1‑140 (MQ=255)                                              tGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTc                                                                              >  2:403744/1‑140 (MQ=255)                                              tGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTc                                                                              >  2:1342455/1‑140 (MQ=255)                                                  cgccgcCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGa                                                                          <  2:1192014/140‑1 (MQ=255)                                                   gccgccGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGAt                                                                         >  1:1131067/1‑140 (MQ=255)                                                   gccgccGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGAt                                                                         >  2:83523/1‑140 (MQ=255)                                                    ccgccgTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATc                                                                        <  2:1131067/140‑1 (MQ=255)                                                              gcaAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCa                                                              <  2:1200982/140‑1 (MQ=255)                                                                 aaTCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTg                                                           >  2:1104714/1‑140 (MQ=255)                                                                    cTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACa                                                        >  2:1201536/1‑140 (MQ=255)                                                                                   aCAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGc                                         <  1:518392/140‑1 (MQ=255)                                                                                   aCAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGc                                         <  1:794259/140‑1 (MQ=255)                                                                                      gCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGcttctt                                              <  2:139280/132‑1 (MQ=255)                                                                                      gCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGcttctt                                              >  1:139280/1‑132 (MQ=255)                                                                                      gCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTgg                                      >  1:108681/1‑140 (MQ=255)                                                                                      gCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTgg                                      >  1:961066/1‑140 (MQ=255)                                                                                        gtggtgTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGgcg                                    >  2:975867/1‑140 (MQ=255)                                                                                        gtggtgTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGgcg                                    <  2:317550/140‑1 (MQ=255)                                                                                        gtggtgTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGgcg                                    >  2:755049/1‑140 (MQ=255)                                                                                        gtggtgTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAAGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGgcg                                    >  2:952585/1‑140 (MQ=255)                                                                                         tggtgTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGgcgc                                   >  1:669409/1‑140 (MQ=255)                                                                                          ggtgTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCa                                  >  1:1246369/1‑140 (MQ=255)                                                                                          ggtgTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAAGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCa                                  <  1:952585/140‑1 (MQ=255)                                                                                                cGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAg                            >  1:282618/1‑140 (MQ=255)                                                                                                     cGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATc                       <  1:403744/140‑1 (MQ=255)                                                                                                          gTAATGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGAt                  <  1:1342455/140‑1 (MQ=255)                                                                                                              tGTTCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTtgt              <  2:628822/140‑1 (MQ=255)                                                                                                                ttCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTTGTgg            >  1:1246681/1‑140 (MQ=255)                                                                                                                ttCTGGCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTTGTgg            <  1:1104714/140‑1 (MQ=255)                                                                                                                     gCATTTGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTTGTGGCACGt       >  2:89597/1‑140 (MQ=255)                                                                                                                          tGGTCAGCGCGGTTGGGCGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTTGTGGCACGTACATg  >  1:215601/1‑140 (MQ=255)                                                                                                                          tGGTCAGCGCGGTTGCGCGCACGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAACTTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTTGTGGCACGTACATg  >  2:663082/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                            ATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCGCAGAGCAGGCATCAGGATTTGTGGCACGTACATG  >  NC_000913/1318114‑1318374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑