Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,361,250	C→T	A44V (GCG→GTG)	puuD →	gamma‑glutamyl‑gamma‑aminobutyrate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,361,250	0	C	T	100.0%	320.8 / NA	89	A44V (GCG→GTG)	puuD	gamma‑glutamyl‑gamma‑aminobutyrate hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/44);  total (45/44)

TGAGCATGGAAAATATAATGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGCGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCC  >  NC_000913/1361115‑1361383                                                                                                                                        |                                                                                                                                      tGAGCATGGAAAATATAATGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTa                                                                                                                                   >  2:491990/1‑140 (MQ=255) tGAGCATGGAAAATATAATGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTa                                                                                                                                   >  1:930962/1‑140 (MQ=255)   aGCATGGAAAATATAATGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTAcc                                                                                                                                 >  2:561203/1‑140 (MQ=255)   aGCATGGAAAATATAATGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTAcc                                                                                                                                 >  1:42314/1‑140 (MQ=255)         gAAAATATAATGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATgc                                                                                                                           >  2:266611/1‑140 (MQ=255)           aaaTATAATGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATgcgc                                                                                                                         <  2:199709/140‑1 (MQ=255)              tataATGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTgg                                                                                                                      <  2:385360/140‑1 (MQ=255)              tataATGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTgg                                                                                                                      <  2:907351/140‑1 (MQ=255)               ataATGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGc                                                                                                                     <  2:158287/140‑1 (MQ=255)                  aTGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGa                                                                                                                  >  2:499885/1‑140 (MQ=255)                  aTGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGa                                                                                                                  <  2:167840/140‑1 (MQ=255)                  aTGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGa                                                                                                                  >  1:258792/1‑140 (MQ=255)                   tGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGaa                                                                                                                 <  2:95019/140‑1 (MQ=255)                   tGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGaa                                                                                                                 <  2:1103295/140‑1 (MQ=255)                   tGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGaa                                                                                                                 <  2:807623/140‑1 (MQ=255)                        aaTCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTgg                                                                                                                      >  1:1041769/1‑130 (MQ=255)                        aaTCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTgg                                                                                                                      <  2:1041769/130‑1 (MQ=255)                        aaTCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTc                                                                                                            <  1:266611/140‑1 (MQ=255)                        aaTCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTc                                                                                                            >  1:1361628/1‑140 (MQ=255)                          tCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCGCATGCGCTGGCGGAACCGTCAt                                                                                                          <  1:561203/140‑1 (MQ=255)                          tCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCAt                                                                                                          >  2:1004454/1‑140 (MQ=255)                                        tCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACtt                                                                                            >  1:1284211/1‑140 (MQ=255)                                           tAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTg                                                                                         >  2:755637/1‑140 (MQ=255)                                           tAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTg                                                                                         >  1:590172/1‑140 (MQ=255)                                            aaTGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGc                                                                                        <  2:329621/140‑1 (MQ=255)                                             aTGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGcc                                                                                       >  2:179923/1‑140 (MQ=255)                                                tgCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGaa                                                                                    >  2:1284498/1‑140 (MQ=255)                                                  cAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACtttt                                                                                          >  1:813287/1‑132 (MQ=255)                                                  cAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACtttt                                                                                          <  2:813287/132‑1 (MQ=255)                                                     gAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCg                                                                               <  2:42314/140‑1 (MQ=255)                                                      aaCAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGa                                                                              <  2:493288/140‑1 (MQ=255)                                                      aaCAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGa                                                                              <  2:1361628/140‑1 (MQ=255)                                                         aGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATgg                                                                           <  1:1004454/140‑1 (MQ=255)                                                          ggCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGc                                                                          >  1:1383283/1‑140 (MQ=255)                                                          ggCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGc                                                                          >  2:1333678/1‑140 (MQ=255)                                                          ggCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGc                                                                          >  1:1179003/1‑140 (MQ=255)                                                          ggCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGc                                                                          >  2:446127/1‑140 (MQ=255)                                                              tAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCAttt                                                                      <  2:768851/140‑1 (MQ=255)                                                                aGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGc                                                                          <  2:676784/134‑1 (MQ=255)                                                                aGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGc                                                                          >  1:676784/1‑134 (MQ=255)                                                                     cATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTcc                                                               >  2:1102320/1‑140 (MQ=255)                                                                     cATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTcc                                                               >  2:184389/1‑140 (MQ=255)                                                                       tGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTg                                                             <  2:80753/140‑1 (MQ=255)                                                                       tGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTg                                                             >  1:936230/1‑140 (MQ=255)                                                                       tGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTg                                                             <  1:361870/140‑1 (MQ=255)                                                                       tGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTg                                                             >  2:1177493/1‑140 (MQ=255)                                                                        gCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTgg                                                            <  2:1179003/140‑1 (MQ=255)                                                                         cGACCCAGACTCTGCAAGAAAAGTGCCTGAATGGCATCATCCATGCAGGAGGCTTGCCTATTGTGCTGCCCCATGCGATGGCGGAGCCGACATCACTTCAACAACTTTAGCCGAAACTGGATGGCATTTATCTTCCTGGt                                                           <  2:234390/140‑1 (MQ=255)                                                                         cGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGt                                                           <  2:611361/140‑1 (MQ=255)                                                                         cGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGt                                                           <  2:68417/140‑1 (MQ=255)                                                                         cGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGt                                                           <  1:184389/140‑1 (MQ=255)                                                                         cGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGt                                                           <  1:1333678/140‑1 (MQ=255)                                                                         cGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGt                                                           >  1:886795/1‑140 (MQ=255)                                                                         cGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGt                                                           <  2:258792/140‑1 (MQ=255)                                                                              cAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTcc                                                      <  2:1195867/140‑1 (MQ=255)                                                                                   tctGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCa                                                 <  1:755637/140‑1 (MQ=255)                                                                                     tGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAAt                                               >  1:618222/1‑140 (MQ=255)                                                                                     tGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAAt                                               >  2:916505/1‑140 (MQ=255)                                                                                     tGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAAt                                               >  1:883018/1‑140 (MQ=255)                                                                                      gCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGt                                                           >  1:561299/1‑127 (MQ=255)                                                                                      gCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGt                                                           >  2:85827/1‑127 (MQ=255)                                                                                      gCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGt                                                           <  2:561299/127‑1 (MQ=255)                                                                                      gCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGt                                                           <  1:85827/127‑1 (MQ=255)                                                                                      gCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTAGTAGTCCCAGCAAtg                                              >  2:940497/1‑140 (MQ=255)                                                                                      gCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCGATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACACGATGGCATTTATCTTCCTGGTAGTCCCAGCAAtg                                              >  2:962502/1‑140 (MQ=255)                                                                                         aGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGc                                           <  2:513323/140‑1 (MQ=255)                                                                                          gAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCa                                          <  1:920733/140‑1 (MQ=255)                                                                                          gAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCa                                          <  2:883018/140‑1 (MQ=255)                                                                                            aaaGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGc                                        <  1:916505/140‑1 (MQ=255)                                                                                            aaaGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGc                                        <  1:1284498/140‑1 (MQ=255)                                                                                            aaaGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGc                                        <  2:618222/140‑1 (MQ=255)                                                                                            aaaGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCACAGCAATGTGCAGc                                        <  1:491990/140‑1 (MQ=255)                                                                                            aaaGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTAGTAGTCCCAGCAATGTGCAGc                                        <  1:940497/140‑1 (MQ=255)                                                                                              aGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCg                                      <  1:1177493/140‑1 (MQ=255)                                                                                                 aCCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCAc                                   >  2:1128615/1‑140 (MQ=255)                                                                                                 aCCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCAc                                   >  1:967653/1‑140 (MQ=255)                                                                                                     gAATGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCtat                               >  2:450105/1‑140 (MQ=255)                                                                                                      aaTGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCtata                              >  1:883667/1‑140 (MQ=255)                                                                                                        tGCCATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATg                            >  2:713657/1‑140 (MQ=255)                                                                                                          ccATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGt                          >  1:809184/1‑140 (MQ=255)                                                                                                          ccATCATCCATGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGt                          <  1:962502/140‑1 (MQ=255)                                                                                                                    tGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCg                >  2:1136332/1‑140 (MQ=255)                                                                                                                    tGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCg                >  1:1353192/1‑140 (MQ=255)                                                                                                                    tGCAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCg                >  2:891567/1‑140 (MQ=255)                                                                                                                      cAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGAt              <  1:1315322/140‑1 (MQ=255)                                                                                                                      cAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGAt              <  2:1383283/140‑1 (MQ=255)                                                                                                                      cAGGCGGCTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGAt              <  1:309902/140‑1 (MQ=255)                                                                                                                         gcggcTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGATGAg           >  2:619610/1‑140 (MQ=255)                                                                                                                           ggcTTGCCTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGATGAGcc         >  1:311992/1‑140 (MQ=255)                                                                                                                                  cTATTGTGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGcc  >  2:661070/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      TGAGCATGGAAAATATAATGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGCGACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCATGCAGGCGGCTTGCCTATTGCGCTACCACATGCGCTGGCGGAACCGTCATTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTGGTAGTCCCAGCAATGTGCAGCCGCACCTATATGGTGAAAACGGCGATGAGCCTGACGCC  >  NC_000913/1361115‑1361383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑