Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 212,602 C→T A91V (GCC→GTC)  tilS → tRNA(Ile)‑lysidine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913212,6020CT98.1% 160.6 / NA 52A91V (GCC→GTC) tilStRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (26/25);  minor base G (1/0);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTATTCATGTGCATCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCG  >  NC_000913/212465‑212736
                                                                                                                                         |                                                                                                                                      
cgcTATTCATGTGCATCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTTAGGtcc                                                                                                                                      >  2:885023/1‑140 (MQ=255)
         tgtgCATCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCggc                                                                                                                             <  2:168771/140‑1 (MQ=255)
              aTCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCggcaggca                                                                                                                        <  1:694797/140‑1 (MQ=255)
               tCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGTACGCGTACAACTTTCGCAAGAAGGGCTGGGGATTGAGGTCCAGGCGCGGCCGGCAc                                                                                                                       >  2:178015/1‑140 (MQ=255)
               tCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCAc                                                                                                                       >  1:961571/1‑140 (MQ=255)
               tCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCAc                                                                                                                       >  2:490811/1‑140 (MQ=255)
               tCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGCGCCCCGGGTGGTCGAACGCGTACAACATGCGCAAAAAGGACTGGGCGTTGAGGTCCAAGCGCGGCAGGCAc                                                                                                                       >  2:755130/1‑140 (MQ=255)
                cACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACg                                                                                                                      >  2:1076661/1‑140 (MQ=255)
                   ggTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTa                                                                                                                   >  2:795363/1‑140 (MQ=255)
                     tttAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATc                                                                                                                 <  1:469584/140‑1 (MQ=255)
                       tAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAg                                                                                                               >  1:906077/1‑140 (MQ=255)
                          gTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCa                                                                                                            <  1:680921/140‑1 (MQ=255)
                           tGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCAt                                                                                                           <  2:37722/140‑1 (MQ=255)
                             ccAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCAttt                                                                                                         >  2:262504/1‑140 (MQ=255)
                               aaTGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGc                                                                                                       >  1:45865/1‑140 (MQ=255)
                                     gATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGGGGGCGAACGCGCACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCACGCGCGGCAGGCCCGTTGTTGGGGATTTGTCCGCAc                                                                                                 >  2:138547/1‑140 (MQ=255)
                                            gggTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCttgttg                                                                                          >  1:391741/1‑140 (MQ=255)
                                              gTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGcc                                                                                        <  1:138547/140‑1 (MQ=255)
                                              gTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGcc                                                                                        <  1:490811/140‑1 (MQ=255)
                                              gTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGcc                                                                                        <  1:820435/140‑1 (MQ=255)
                                              gTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGcc                                                                                        <  1:1076661/140‑1 (MQ=255)
                                                 aCGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGGATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCCTGTTGCCCgg                                                                                     >  2:562481/1‑140 (MQ=255)
                                                 aCGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCgg                                                                                     <  2:1170769/140‑1 (MQ=255)
                                                  cGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGGCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCCTGTTGCCCggg                                                                                    >  2:1162819/1‑139 (MQ=255)
                                                   gCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTg                                                                                   <  1:795363/140‑1 (MQ=255)
                                                   gCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTg                                                                                   <  1:595728/140‑1 (MQ=255)
                                                   gCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTg                                                                                   <  2:451333/140‑1 (MQ=255)
                                                   gCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTg                                                                                   <  1:1041214/140‑1 (MQ=255)
                                                   gCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTg                                                                                   <  1:262504/140‑1 (MQ=255)
                                                       tGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGt                                                                               >  1:106117/1‑140 (MQ=255)
                                                        gCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCCCACCTTGTTGCCCGGGGAAGTg                                                                              >  2:52654/1‑140 (MQ=255)
                                                                   tGCCAACAGTGGCATGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACcgc                                                                   >  2:821130/1‑140 (MQ=255)
                                                                        aCAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAAc                                                              <  2:106117/140‑1 (MQ=255)
                                                                           gTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACAtc                                                           >  1:802518/1‑140 (MQ=255)
                                                                               cAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTTCCCGCACCTTTTTGCCCGGGGGAGTGCTGGTCACCCCGCAACATCTcga                                                       >  2:344313/1‑140 (MQ=255)
                                                                                 ggTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTcgacg                                                     <  1:1198061/140‑1 (MQ=255)
                                                                                   tGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTTCCCGCCCCCTGTTGCCCGGGGGAGGGCTGGTCACCCCGCAACAACTCGACGAt                                                   >  2:556574/1‑140 (MQ=255)
                                                                                    gCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATc                                                  >  1:1237846/1‑140 (MQ=255)
                                                                                    gCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATc                                                  <  1:344313/140‑1 (MQ=255)
                                                                                    gCCGCTGGGGGTCGAACGCGTACAACTTGCGCAAGAAAGACTGGGCATTGAGGGCCAGGCGCGGCAGGCACCTTATCAGGCATTTGCCCCCCCCCTGTTGCCCCGTGGAGGGCTGGGCCCCCCGCCACAACTCGACGGTc                                                  >  2:1083620/1‑140 (MQ=255)
                                                                                      cgatggtggTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCaa                                                >  1:1088793/4‑140 (MQ=255)
                                                                                      cGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCaa                                                >  1:1230370/1‑140 (MQ=255)
                                                                                      cGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCaa                                                >  2:435819/1‑140 (MQ=255)
                                                                                                     gcgTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTctg                                 >  2:1145810/1‑140 (MQ=255)
                                                                                                      cgTACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTctgc                                >  2:1108743/1‑140 (MQ=255)
                                                                                                      catACAACTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTctgc                                >  2:819056/3‑140 (MQ=255)
                                                                                                            aCTTGCGCAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGgcgc                          <  2:391741/140‑1 (MQ=255)
                                                                                                                 cgcAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTaaaa                     >  1:1070167/1‑140 (MQ=255)
                                                                                                                 cgcAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTaaaa                     >  2:1347234/1‑140 (MQ=255)
                                                                                                                 cgcAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTaaaa                     >  1:268273/1‑140 (MQ=255)
                                                                                                                   cAAGAAGGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAAcg                   <  1:1347234/140‑1 (MQ=255)
                                                                                                                    aagaagGACTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTCAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAAcgc                  <  2:1230370/140‑1 (MQ=255)
                                                                                                                            cTGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTgg          <  1:1145810/140‑1 (MQ=255)
                                                                                                                             tGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGc         <  2:322840/140‑1 (MQ=255)
                                                                                                                             tGGGCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGc         <  1:328786/140‑1 (MQ=255)
                                                                                                                                gCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTgg          <  2:1050207/136‑1 (MQ=255)
                                                                                                                                gCATTGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTgg          >  1:1050207/1‑136 (MQ=255)
                                                                                                                                    tGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCg  >  2:1176235/1‑140 (MQ=255)
                                                                                                                                    tGAGGTCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCg  >  1:919634/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                      
CGCTATTCATGTGCATCACGGTTTAAGTGCCAATGCCGATGCCTGGGTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTACAACTTGCGCAAGAAGGACTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCG  >  NC_000913/212465‑212736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑