Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,492,842	G→A	A125T (GCC→ACC)	trg →	methyl‑accepting chemotaxis protein Trg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,492,842	0	G	A	100.0%	297.3 / NA	85	A125T (GCC→ACC)	trg	methyl‑accepting chemotaxis protein Trg
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/44);  total (41/44)

AGGCCGGGGCTGCGAGTCGTATTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAGCCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAGTGAGCAGATCCGACCGCTGGATAATG  >  NC_000913/1492705‑1492977                                                                                                                                          |                                                                                                                                        aGGCCGGGGCTGCGAGTCGTATTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAAcc                                                                                                                                       >  2:1338339/1‑140 (MQ=255) aGGCCGGGGCTGCGAGTCGTATTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAAcc                                                                                                                                       >  2:780176/1‑140 (MQ=255)   gCCGGGGCTGCGAGTCGTATTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCt                                                                                                                                     >  2:1395068/1‑140 (MQ=255)   gCCGGGGCTGCGAGTCGTATTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCt                                                                                                                                     >  1:865054/1‑140 (MQ=255)   gCCGGGGCTGCGAGTCGTATTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCt                                                                                                                                     >  1:1265008/1‑140 (MQ=255)   gCCGGGGCTGCGAGTCGTATTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCt                                                                                                                                     >  1:1340654/1‑140 (MQ=255)   gCCGGGGCTGCGAGTCGTATTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCt                                                                                                                                     <  2:566533/140‑1 (MQ=255)   gCCGGGGCTGCGAGTCGTATTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTAATGAAACCCt                                                                                                                                     >  1:960660/1‑140 (MQ=255)        ggCTGCGAGTCGTATTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGaca                                                                                                                                <  2:145306/140‑1 (MQ=255)        ggCTGCGAGTCGTATTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGaca                                                                                                                                <  1:610429/140‑1 (MQ=255)        ggCTGCGAGTCGTATTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGaca                                                                                                                                >  2:763194/1‑140 (MQ=255)        ggCTGCGAGTCGTATTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGaca                                                                                                                                <  2:703826/140‑1 (MQ=255)         gCTGCGAGTCGTATTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGacac                                                                                                                               <  1:1332658/140‑1 (MQ=255)         gCTGCGAGTCGTATTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGacac                                                                                                                               <  1:169447/140‑1 (MQ=255)         gCTGCGAGTCGTATTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGacac                                                                                                                               <  2:740751/140‑1 (MQ=255)             cGAGTCGTATTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGaa                                                                                                                           >  1:670307/1‑140 (MQ=255)                   gTATTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGATTTaatt                                                                                                                     <  2:694539/140‑3 (MQ=255)                     aTTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCa                                                                                                                   <  2:84216/140‑1 (MQ=255)                            aaaTGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCttt                                                                                                            <  1:245817/140‑1 (MQ=255)                            aaaTGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCttt                                                                                                            <  2:1340654/140‑1 (MQ=255)                              aTGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCa                                                                                                          >  2:533547/1‑140 (MQ=255)                              aTGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCa                                                                                                          >  2:536742/1‑140 (MQ=255)                                ggAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAgg                                                                                                        <  2:1265008/140‑1 (MQ=255)                                   aGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCtt                                                                                                     >  2:358966/1‑140 (MQ=255)                                       aTGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTatat                                                                                                 <  1:767024/140‑1 (MQ=255)                                         gAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCa                                                                                               <  2:717421/140‑1 (MQ=255)                                         gAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCa                                                                                               <  2:670307/140‑1 (MQ=255)                                            gCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACgg                                                                                            >  1:122664/1‑140 (MQ=255)                                            gCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACgg                                                                                            <  1:1395068/140‑1 (MQ=255)                                            gCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACgg                                                                                            <  2:289209/140‑1 (MQ=255)                                              gAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGt                                                                                          <  2:32866/140‑1 (MQ=255)                                               aaaTATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTa                                                                                         <  1:536742/140‑1 (MQ=255)                                                aaTATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTAt                                                                                        <  2:122664/140‑1 (MQ=255)                                                aaTATTGCGCAAGCCGAATCGCTGCTTAAAGAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTCTCAGGCTTATATCACGGGTAt                                                                                        >  2:813213/1‑140 (MQ=255)                                                  tatTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGc                                                                                      <  1:358966/140‑1 (MQ=255)                                                     tGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAAc                                                                                   <  1:780176/140‑1 (MQ=255)                                                     tGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAAc                                                                                   <  1:66786/140‑1 (MQ=255)                                                     tGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAAc                                                                                   <  1:1302376/140‑1 (MQ=255)                                                       cgcAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAAcc                                                                                  >  2:15488/1‑139 (MQ=255)                                                       cgcAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAAcc                                                                                  <  1:15488/139‑1 (MQ=255)                                                       cgcAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAAc                                                                                   >  1:1133635/1‑138 (MQ=255)                                                       cgcAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAAc                                                                                   <  2:1133635/138‑1 (MQ=255)                                                            gCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAAc                                                                                   >  1:1353997/1‑133 (MQ=255)                                                            gCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAAc                                                                                   <  2:1353997/133‑1 (MQ=255)                                                            gCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGtt                                                                            >  1:432459/1‑140 (MQ=255)                                                            gCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGtt                                                                            >  1:1370606/1‑140 (MQ=255)                                                                 aTCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAat                                                                       >  1:1191579/1‑140 (MQ=255)                                                                     gagaTTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGc                                                                   <  2:432459/140‑1 (MQ=255)                                                                      agaTTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGcc                                                                  >  2:332032/1‑140 (MQ=255)                                                                      agaTTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGcc                                                                  >  1:1104549/1‑140 (MQ=255)                                                                        aTTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCaa                                                                >  1:1233114/1‑140 (MQ=255)                                                                              cAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATgg                                                          <  1:649893/140‑1 (MQ=255)                                                                                  cGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATg                                                      >  1:643470/1‑140 (MQ=255)                                                                                  cGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATg                                                      >  2:661410/1‑140 (MQ=255)                                                                                        aaGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGaa                                                <  1:1366376/140‑1 (MQ=255)                                                                                         aGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAg                                               <  1:533547/140‑1 (MQ=255)                                                                                         aGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAg                                               <  2:1233114/140‑1 (MQ=255)                                                                                             tATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGAt                                           >  2:1006224/1‑140 (MQ=255)                                                                                               tCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTa                                         >  2:496606/1‑140 (MQ=255)                                                                                                    cTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTatcaat                                    >  2:29716/1‑140 (MQ=255)                                                                                                     ttATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTatcaatc                                   >  1:729144/1‑140 (MQ=255)                                                                                                     ttATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTatcaatc                                   >  2:832653/1‑140 (MQ=255)                                                                                                      tATCAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTatcaatca                                  >  1:501555/1‑140 (MQ=255)                                                                                                         cAGAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGa                               >  1:878831/1‑140 (MQ=255)                                                                                                           gAATCGACCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCACGaaa                             <  2:643470/140‑1 (MQ=255)                                                                                                            aaTCGACCGGTGAAGACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAg                            >  1:22462/1‑140 (MQ=255)                                                                                                                 aCCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATg                                >  2:538207/1‑131 (MQ=255)                                                                                                                 aCCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATg                                <  1:538207/131‑1 (MQ=255)                                                                                                                 aCCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAGTGAGc                       >  2:366811/1‑140 (MQ=255)                                                                                                                 aCCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAGTGAGc                       >  2:861929/1‑140 (MQ=255)                                                                                                                 aCCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAGTGAGc                       >  2:13967/1‑140 (MQ=255)                                                                                                                 aCCGGTGAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAGTGAGc                       >  1:281997/1‑140 (MQ=255)                                                                                                                       gAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAGTGAGCAGATcc                 <  1:1006224/140‑1 (MQ=255)                                                                                                                       gAAAACACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAGTGAGCAGATcc                 <  1:832653/140‑1 (MQ=255)                                                                                                                          aaCACCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAGTGAGCAGATCCGAc              <  2:865054/140‑1 (MQ=255)                                                                                                                            cacCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAGTGAGCAGATCCGACCg            <  1:763194/140‑1 (MQ=255)                                                                                                                            cacCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAGTGAGCAGATCCGACCg            <  1:1338339/140‑1 (MQ=255)                                                                                                                             acCTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAGTGAGCAGATCCGACCGc           >  2:812784/1‑140 (MQ=255)                                                                                                                              ccTGCTGATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAGTGAGCAGATCCGACCGCt          >  2:295961/1‑140 (MQ=255)                                                                                                                                  ctgATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAGTGAGCAGATCCGACCg            <  2:42154/134‑1 (MQ=255)                                                                                                                                  ctgATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAGTGAGCAGATCCGACCg            >  1:42154/1‑134 (MQ=255)                                                                                                                                  ctgATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAGTGAGCAGATCCGACCGCTGGAt      <  1:282975/140‑1 (MQ=255)                                                                                                                                  ctgATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAGTGAGCAGATCCGACCGCTGGAt      <  1:332032/140‑1 (MQ=255)                                                                                                                                  ctgATGAAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTCGAAGCGATTATCAATCATGAAAGTGAGCAGATCCGACCGCTGGAt      <  2:960660/140‑1 (MQ=255)                                                                                                                                     atgaAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAGTGAGCAGATCCGACCGCTGGATAAt   >  1:251079/1‑140 (MQ=255)                                                                                                                                      tgaAACCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAGTGAGCAGATCCGACCGCTGGATAATg  <  1:661410/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        AGGCCGGGGCTGCGAGTCGTATTGCGGAAATGGAAGCAATGAAGCGAAATATTGCGCAAGCCGAATCGGAGATTAAACAGTCGCAGCAAGGTTATCGTGCTTATCAGAATCGACCGGTGAAAACACCTGCTGATGAAGCCCTCGACACTGAATTAAATCAACGCTTTCAGGCTTATATCACGGGTATGCAACCTATGTTGAAATATGCCAAAAATGGCATGTTTGAAGCGATTATCAATCATGAAAGTGAGCAGATCCGACCGCTGGATAATG  >  NC_000913/1492705‑1492977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑