Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,507,873	A→G	T365A (ACC→GCC)	rlhA →	23S rRNA 5‑hydroxycytidine C2501 synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,507,873	0	A	G	100.0%	235.7 / NA	65	T365A (ACC→GCC)	rlhA	23S rRNA 5‑hydroxycytidine C2501 synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/29);  total (36/29)

GGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGT  >  NC_000913/1507740‑1508007                                                                                                                                      |                                                                                                                                       ggTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGgag                                                                                                                                  <  2:775130/140‑1 (MQ=255) ggTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGgag                                                                                                                                  <  2:342793/140‑1 (MQ=255) ggTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGgag                                                                                                                                  <  1:1159203/140‑1 (MQ=255) ggTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGgag                                                                                                                                  <  1:1304774/140‑1 (MQ=255)         aaGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAg                                                                                                                          >  2:1295791/1‑140 (MQ=255)         aaGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAg                                                                                                                          >  1:272500/1‑140 (MQ=255)            tagtGAAAGTGGCGAAAGATCATCTCGTTGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTAc                                                                                                                       >  2:1375797/1‑140 (MQ=255)            tagtGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTAc                                                                                                                       <  2:294892/140‑1 (MQ=255)            tagtGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTAc                                                                                                                       <  1:469240/140‑1 (MQ=255)             agtGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTAcc                                                                                                                      <  2:1132750/140‑1 (MQ=255)             agtGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTAcc                                                                                                                      <  2:1151308/140‑1 (MQ=255)               tGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACcgc                                                                                                                    >  1:898244/1‑140 (MQ=255)                  aaGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTc                                                                                                                 <  1:806369/140‑1 (MQ=255)                      ggCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGc                                                                                                             >  1:1103529/1‑140 (MQ=255)                       gCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGTCGGAGAAAATCAGTACCGCGTCTGGcc                                                                                                            >  2:875905/1‑140 (MQ=255)                       gCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGcc                                                                                                            <  1:1333056/140‑1 (MQ=255)                       gCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGcc                                                                                                            >  1:48005/1‑140 (MQ=255)                       gCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGcc                                                                                                            >  2:1049246/1‑140 (MQ=255)                                catcTCGTTGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAAt                                                                                                   <  1:1375797/140‑1 (MQ=255)                                 atcTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGaa                                                                                                     <  1:1069630/137‑1 (MQ=255)                                 atcTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGaa                                                                                                     >  2:1069630/1‑137 (MQ=255)                                 atcTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATg                                                                                                  >  1:451959/1‑140 (MQ=255)                                     cGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCcag                                                                                              >  2:1110682/1‑140 (MQ=255)                                             ccGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGTCCAATGAAATACCAGCAGATTTg                                                                                      >  2:1092857/1‑140 (MQ=255)                                             ccGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTg                                                                                      >  2:1061508/1‑140 (MQ=255)                                                     gAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAAt                                                                              <  2:171808/140‑1 (MQ=255)                                                      aGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAAtt                                                                             >  1:645173/1‑140 (MQ=255)                                                        ccACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCg                                                                           >  1:104161/1‑140 (MQ=255)                                                         cACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGt                                                                          <  1:456903/140‑1 (MQ=255)                                                           cTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTcc                                                                        <  1:1173220/140‑1 (MQ=255)                                                            tGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCca                                                                       >  2:740270/1‑140 (MQ=255)                                                            tGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCca                                                                       >  2:17536/1‑140 (MQ=255)                                                              gCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTc                                                                         >  1:971017/1‑136 (MQ=255)                                                              gCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTc                                                                         <  2:971017/136‑1 (MQ=255)                                                              gCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCcaca                                                                     <  1:77549/140‑1 (MQ=255)                                                                 aaTGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCa                                                                  >  2:834777/1‑140 (MQ=255)                                                                  aTGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCAc                                                                 <  1:905211/140‑1 (MQ=255)                                                                     gCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCa                                                              >  2:387499/1‑140 (MQ=255)                                                                       gATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGTCCAATGAAATACCAGCAGATTTGCACAAAATTCGTCCACATCACCCACt                                                            <  1:1092857/140‑1 (MQ=255)                                                                       gATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACt                                                            <  1:1049246/140‑1 (MQ=255)                                                                       gATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACt                                                            <  2:353469/140‑1 (MQ=255)                                                                        aTGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTa                                                           >  2:233723/1‑140 (MQ=255)                                                                        aTGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTa                                                           >  1:961797/1‑140 (MQ=255)                                                                        aTGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTa                                                           >  2:117982/1‑140 (MQ=255)                                                                        aTGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTa                                                           <  2:1103529/140‑1 (MQ=255)                                                                              tGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGt                                                     >  2:434008/1‑140 (MQ=255)                                                                               gAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTa                                                    >  1:1007729/1‑140 (MQ=255)                                                                               gAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTa                                                    <  2:48005/140‑1 (MQ=255)                                                                                  cGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATc                                                 <  1:1295791/140‑1 (MQ=255)                                                                                     gtTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTg                                              >  2:100506/1‑140 (MQ=255)                                                                                      ttGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTa                                                           >  2:770282/1‑126 (MQ=255)                                                                                      ttGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTa                                                           <  1:770282/126‑1 (MQ=255)                                                                                          ttAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCAt                                         >  2:150024/1‑140 (MQ=255)                                                                                          ttAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCAt                                         >  1:841290/1‑140 (MQ=255)                                                                                              aCGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACt                                     >  2:866633/1‑140 (MQ=255)                                                                                               cGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTg                                    <  2:67034/140‑1 (MQ=255)                                                                                                   aaGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTg                                    <  2:702235/136‑1 (MQ=255)                                                                                                   aaGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTg                                    >  1:702235/1‑136 (MQ=255)                                                                                                           ggTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACt                        <  2:272500/140‑1 (MQ=255)                                                                                                           ggTTTTCGTGCCAATACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACt                        <  1:233723/140‑1 (MQ=255)                                                                                                                         tACGGTCGAGAAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCa          >  2:868292/1‑140 (MQ=255)                                                                                                                                gagaAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACg   >  1:1261321/1‑140 (MQ=255)                                                                                                                                 agaAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGt  >  1:91029/1‑140 (MQ=255)                                                                                                                                 agaAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGt  >  2:1141815/1‑140 (MQ=255)                                                                                                                                 agaAAGCCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGt  >  2:993303/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       GGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGT  >  NC_000913/1507740‑1508007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑