Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,945,042	T→C	E105E (GAA→GAG)	ruvB ←	Holliday junction branch migration complex subunit RuvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,945,042	0	T	C	100.0%	239.1 / NA	66	E105E (GAA→GAG)	ruvB	Holliday junction branch migration complex subunit RuvB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/23);  total (43/23)

TTTTAATGGAGCGTGCCGCCGGACCTTCACCAATCATGATATCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAA  >  NC_000913/1944909‑1945177                                                                                                                                      |                                                                                                                                        ttttAATGGAGCGTGCCGCCGGACCTTCACCAATCATGATATCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAgg                                                                                                                                   <  2:1111901/140‑1 (MQ=255)              tGCCGCCGGACCTTCACCAATCATGATATCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCg                                                                                                                      >  1:1114919/1‑140 (MQ=255)                               cAATCATGATATCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATcgcccgcc                                                                                                     <  1:477959/140‑1 (MQ=255)                                aaTCATGATATCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCt                                                                                                    <  2:1386237/140‑1 (MQ=255)                                 aTCATGATATCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATcgcccg                                                                                                       >  2:178102/1‑136 (MQ=255)                                 aTCATGATATCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATcgcccg                                                                                                       <  1:178102/136‑1 (MQ=255)                                 aTCATGATATCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCtt                                                                                                   >  2:1218681/1‑140 (MQ=255)                                         atCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCa                                                                                           <  2:1179347/140‑1 (MQ=255)                                         atCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCa                                                                                           >  1:35651/1‑140 (MQ=255)                                          tCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCAc                                                                                          <  1:1218681/140‑1 (MQ=255)                                           ccAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCAcc                                                                                         <  1:893539/140‑1 (MQ=255)                                            cAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCg                                                                                        >  2:1288266/1‑140 (MQ=255)                                                tGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGAcc                                                                                    >  2:387233/1‑140 (MQ=255)                                                  gTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAg                                                                                  <  2:1116292/140‑1 (MQ=255)                                                    aGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGaa                                                                                >  2:1214458/1‑140 (MQ=255)                                                     gTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAg                                                                               <  1:184828/140‑1 (MQ=255)                                                        ttCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCg                                                                            >  1:533302/1‑140 (MQ=255)                                                           cATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGc                                                                         >  1:527297/1‑140 (MQ=255)                                                             ttGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGt                                                                       >  1:36703/1‑140 (MQ=255)                                                             ttGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGt                                                                       <  2:335138/140‑1 (MQ=255)                                                                   ggTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTa                                                                 >  1:144855/1‑140 (MQ=255)                                                                     tACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAAc                                                               <  2:35651/140‑1 (MQ=255)                                                                     tACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAAc                                                               <  2:527297/140‑1 (MQ=255)                                                                        aGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGcc                                                            >  1:167511/1‑140 (MQ=255)                                                                          cACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCg                                                                        >  1:347075/1‑126 (MQ=255)                                                                          cACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCg                                                                        <  2:347075/126‑1 (MQ=255)                                                                          cACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCa                                                          >  1:654078/1‑140 (MQ=255)                                                                               cttcAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCa                                                          <  2:35457/135‑1 (MQ=255)                                                                               cttcAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCa                                                          >  1:35457/1‑135 (MQ=255)                                                                               cttcAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCa                                                     >  2:81858/1‑140 (MQ=255)                                                                               cttcAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCa                                                     >  1:648322/1‑140 (MQ=255)                                                                               cttcAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCa                                                     >  2:902747/1‑140 (MQ=255)                                                                                  caacaaCTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATAAACGCCCATTTCATTg                                                  >  2:95445/1‑140 (MQ=255)                                                                                       acTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAAc                                                               >  1:199017/1‑122 (MQ=255)                                                                                       acTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAAc                                                               <  2:199017/122‑1 (MQ=255)                                                                                       acTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGa                                              <  2:1257158/139‑1 (MQ=255)                                                                                       acTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGa                                              >  1:1257158/1‑139 (MQ=255)                                                                                       acTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGAc                                             >  2:1197267/1‑140 (MQ=255)                                                                                       acTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGAGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGAc                                             >  1:288286/1‑140 (MQ=255)                                                                                          ggCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAAt                                          >  2:71030/1‑140 (MQ=255)                                                                                          ggCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAAt                                          >  1:424049/1‑140 (MQ=255)                                                                                          ggCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAAt                                          >  1:1065971/1‑140 (MQ=255)                                                                                                aGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCACCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTgg                                     <  2:127460/139‑1 (MQ=255)                                                                                                aGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCACCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTgg                                     >  1:127460/1‑139 (MQ=255)                                                                                                     gTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCg                               <  1:864529/140‑1 (MQ=255)                                                                                                     gTGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCg                               <  2:167511/140‑1 (MQ=255)                                                                                                      tGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCg                               >  2:1304197/1‑139 (MQ=255)                                                                                                      tGGATCTCATCAATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCg                               <  1:1304197/139‑1 (MQ=255)                                                                                                             catcaATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTa                       <  2:1114919/140‑1 (MQ=255)                                                                                                              atcaATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTAc                      >  1:76894/1‑140 (MQ=255)                                                                                                               tcaATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTAcc                     <  1:902747/140‑1 (MQ=255)                                                                                                                caATAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTAccc                    <  1:71030/140‑1 (MQ=255)                                                                                                                  aTAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCaa                  >  1:960901/1‑140 (MQ=255)                                                                                                                   tAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGTAAGCGTAGTTTTACCCAAc                 >  1:242266/1‑140 (MQ=255)                                                                                                                   tAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAAc                 >  2:1185530/1‑140 (MQ=255)                                                                                                                   tAAACAGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAAc                 >  1:854142/1‑140 (MQ=255)                                                                                                                        aGCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCgg            >  1:1212819/1‑140 (MQ=255)                                                                                                                         gCACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCgga           >  2:596590/1‑140 (MQ=255)                                                                                                                          cACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCggag          >  2:212995/1‑140 (MQ=255)                                                                                                                          cACGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCggag          >  1:917148/1‑140 (MQ=255)                                                                                                                           aCGTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCggagg         >  2:507257/1‑140 (MQ=255)                                                                                                                             gTCATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGAc       >  2:304329/1‑140 (MQ=255)                                                                                                                               cATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCa     >  1:331294/1‑140 (MQ=255)                                                                                                                               cATGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCa     >  2:675596/1‑140 (MQ=255)                                                                                                                                 tGCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCaaa   >  1:1147425/1‑140 (MQ=255)                                                                                                                                  gCGGCTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCaaaa  >  1:671134/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        TTTTAATGGAGCGTGCCGCCGGACCTTCACCAATCATGATATCCAGTTGGTAGTCTTCCATTGCCGGGTACAGCACTTCTTCAACAACTGGCGATAGACGGTGGATCTCATCAATAAACAGCACGTCATGCGGTTCAAGGTTAGTGAGCATCGCAGCCAAATCGCCCGCCTTTTCCAGCACCGGACCAGAAGTCGTGCGTAAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAA  >  NC_000913/1944909‑1945177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑