Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,947,589	G→A	A188V (GCG→GTG)	yebC ←	putative transcriptional regulator YebC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,947,589	0	G	A	100.0%	310.8 / NA	88	A188V (GCG→GTG)	yebC	putative transcriptional regulator YebC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/50);  total (38/50)

GGTAAACTTCCTGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCT  >  NC_000913/1947452‑1947724                                                                                                                                          |                                                                                                                                        ggTAAACTTCCTGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACt                                                                                                                                       >  2:63359/1‑140 (MQ=255)     aaCTTCCTGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCa                                                                                                                                   >  1:231624/1‑140 (MQ=255)       cTTCCTGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCaga                                                                                                                                 >  2:222914/1‑140 (MQ=255)       cTTCCTGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCaga                                                                                                                                 >  2:955421/1‑140 (MQ=255)       cTTCCTGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCaga                                                                                                                                 >  1:410367/1‑140 (MQ=255)          ccTGCACGTCGTCGCAATCTTCCAGTATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCg                                                                                                                              >  2:1059773/1‑140 (MQ=255)          ccTGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCg                                                                                                                              >  2:845462/1‑140 (MQ=255)          ccTGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCg                                                                                                                              >  1:978293/1‑140 (MQ=255)            tGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTc                                                                                                                            >  2:1152827/1‑140 (MQ=255)            tGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTc                                                                                                                            >  1:657753/1‑140 (MQ=255)             gCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTcg                                                                                                                           >  1:1244551/1‑140 (MQ=255)               aCGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTcgcg                                                                                                                         <  1:307826/140‑1 (MQ=255)                 gtcgtcGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCa                                                                                                                       >  2:446996/1‑140 (MQ=255)                 gtcgtcGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCa                                                                                                                       >  2:917279/1‑140 (MQ=255)                  tcgtcgCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTcgc                                                                                                                          <  2:645731/136‑1 (MQ=255)                  tcgtcgCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTcgc                                                                                                                          >  1:645731/1‑136 (MQ=255)                   cgtcgCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACt                                                                                                                     <  1:543821/140‑1 (MQ=255)                    gtcgCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACtt                                                                                                                    >  1:839607/1‑140 (MQ=255)                      cgCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAACCTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTa                                                                                                                  <  2:410367/140‑1 (MQ=255)                       gCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTAc                                                                                                                 <  1:630304/140‑1 (MQ=255)                         aaTCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCTACTTCCAGAGCGTCGCGCACTTTAccc                                                                                                               <  2:775688/140‑1 (MQ=255)                            cTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCAtt                                                                                                            <  1:482605/140‑1 (MQ=255)                                 aGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATttcttc                                                                                                       >  2:654169/1‑140 (MQ=255)                                   cATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTccc                                                                                                     <  2:1256189/140‑1 (MQ=255)                                   cATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTccc                                                                                                     <  2:810177/140‑1 (MQ=255)                                     tatCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCAt                                                                                                   <  2:231624/140‑1 (MQ=255)                                      atCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATg                                                                                                  <  1:884693/140‑1 (MQ=255)                                      atCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATg                                                                                                  <  2:978293/140‑1 (MQ=255)                                      atCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATg                                                                                                  <  2:715708/140‑1 (MQ=255)                                      atCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATg                                                                                                  <  1:654169/140‑1 (MQ=255)                                      atCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATg                                                                                                  <  1:1059773/140‑1 (MQ=255)                                      atCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATg                                                                                                  <  1:63359/140‑1 (MQ=255)                                      atCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATg                                                                                                  <  1:177757/140‑1 (MQ=255)                                       tCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATttcttc                                                                                                       <  1:522272/134‑1 (MQ=255)                                       tCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATttcttc                                                                                                       >  2:522272/1‑134 (MQ=255)                                       tCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGc                                                                                                 >  1:977345/1‑140 (MQ=255)                                       tCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGc                                                                                                 >  1:942172/1‑140 (MQ=255)                                        cGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCa                                                                                                >  1:22291/1‑140 (MQ=255)                                          aTCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAgt                                                                                              >  2:437149/1‑140 (MQ=255)                                             aGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTa                                                                                           >  2:178631/1‑140 (MQ=255)                                               aCGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGa                                                                                         >  1:1290356/1‑140 (MQ=255)                                                        tttCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATcg                                                                                >  1:822007/1‑140 (MQ=255)                                                         ttCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATcgc                                                                               <  1:923181/140‑1 (MQ=255)                                                           cGGGGCGTTTTCTGCATCCATATCAGCTTTGGTAGCCGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCgcgc                                                                             <  2:132499/140‑1 (MQ=255)                                                            ggTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGcc                                                                            <  1:845462/140‑1 (MQ=255)                                                            ggTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGcc                                                                            <  1:222914/140‑1 (MQ=255)                                                            ggTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGcc                                                                            <  2:1101569/140‑1 (MQ=255)                                                            ggTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGcc                                                                            <  2:1244551/140‑1 (MQ=255)                                                              tGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGt                                                                          <  2:790115/140‑1 (MQ=255)                                                                        gCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGt                                                                >  1:1089841/1‑140 (MQ=255)                                                                         cATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTc                                                               <  1:1152827/140‑1 (MQ=255)                                                                                atCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGtcatc                                                                      >  1:518071/1‑126 (MQ=255)                                                                                atCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGtcatc                                                                      <  2:518071/126‑1 (MQ=255)                                                                                atCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGt                                                        >  1:862650/1‑140 (MQ=255)                                                                                 tCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTc                                                       <  2:977345/140‑1 (MQ=255)                                                                                    gCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTc                                                    <  1:461033/140‑1 (MQ=255)                                                                                    gCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTc                                                    <  1:204635/140‑1 (MQ=255)                                                                                     cTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCa                                                   >  1:127425/1‑140 (MQ=255)                                                                                     cTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCa                                                   >  2:203182/1‑140 (MQ=255)                                                                                     cTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCa                                                   >  2:570014/1‑140 (MQ=255)                                                                                      tttGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAg                                                  <  2:389806/140‑1 (MQ=255)                                                                                        tGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCa                                                <  1:955421/140‑1 (MQ=255)                                                                                         ggTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAg                                                  <  1:402572/137‑1 (MQ=255)                                                                                         ggTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAg                                                  >  2:402572/1‑137 (MQ=255)                                                                                           tAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCt                                             <  2:657753/140‑1 (MQ=255)                                                                                               cGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCtt                                         >  2:574753/1‑140 (MQ=255)                                                                                                gggATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTc                                        <  1:178631/140‑1 (MQ=255)                                                                                                    tCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGt                                    >  1:299842/1‑140 (MQ=255)                                                                                                     cATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGtg                                   <  1:604305/140‑1 (MQ=255)                                                                                                     cATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGtg                                   <  2:942172/140‑1 (MQ=255)                                                                                                       tGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGtgct                                 >  1:787273/1‑140 (MQ=255)                                                                                                         gAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCTGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGtgctgc                               <  1:1221414/140‑1 (MQ=255)                                                                                                         gAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGtgctgc                               <  1:1205249/140‑1 (MQ=255)                                                                                                         gAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGtgctgc                               <  1:437149/140‑1 (MQ=255)                                                                                                         gAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGtgctgc                               <  2:644646/140‑1 (MQ=255)                                                                                                           agcTTCCGCGCTGTCTGTTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCtt                             <  2:474900/138‑1 (MQ=255)                                                                                                            aCTTCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTc                            >  2:644522/1‑140 (MQ=255)                                                                                                              ttCCGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCa                          >  2:185458/1‑140 (MQ=255)                                                                                                                ccGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCatg                        >  2:283920/1‑140 (MQ=255)                                                                                                                ccGCGCTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCatg                        <  2:839607/140‑1 (MQ=255)                                                                                                                  gcgcTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCatgat                      <  1:446996/140‑1 (MQ=255)                                                                                                                   cgcTGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCatgatg                     <  1:638320/140‑1 (MQ=255)                                                                                                                      tGTCTGCTTTCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGgtg                  <  2:1089841/140‑1 (MQ=255)                                                                                                                       gTCTGCTTCCAGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGgtgt                 <  1:491252/140‑1 (MQ=255)                                                                                                                                 aGACCTGCCATTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGcc       <  2:862650/140‑1 (MQ=255)                                                                                                                                 aGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGcc       <  2:1290356/140‑1 (MQ=255)                                                                                                                                 aGACCTGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGcc       <  2:1193643/140‑1 (MQ=255)                                                                                                                                      tGCCACTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGCGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTtct  >  1:1306301/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GGTAAACTTCCTGCACGTCGTCGCAATCTTCCAGCATATCGATCAGACGCATCAGTTTCGGTGCGGTTTCTGCATCCATATCAGCTTTGGTAGACGGGATCATGGAAACTTCCGCGCTGTCTGCTTTCAGACCTGCCGCTTCCAGAGCGTCGCGCACTTTACCCATTTCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCT  >  NC_000913/1947452‑1947724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑