Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,996,183	T→C	N217S (AAC→AGC)	sdiA ←	DNA‑binding transcriptional dual regulator SdiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,996,183	0	T	C	100.0%	205.1 / NA	56	N217S (AAC→AGC)	sdiA	DNA‑binding transcriptional dual regulator SdiA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/26);  total (30/26)

AAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTA  >  NC_000913/1996046‑1996318                                                                                                                                          |                                                                                                                                        aaaaCCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGctt                                                                                                                                       >  1:637296/1‑140 (MQ=255)   aaCCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGctttt                                                                                                                                     <  2:1365929/140‑1 (MQ=255)       ggCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCtgg                                                                                                                                 <  1:1021972/140‑1 (MQ=255)        gCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCtggt                                                                                                                                >  1:426560/1‑140 (MQ=255)         cTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCtggtg                                                                                                                               >  1:826745/1‑140 (MQ=255)                  tGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                      >  2:929325/1‑140 (MQ=255)                  tGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                      >  2:1363110/1‑140 (MQ=255)                  tGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                      >  2:1360181/1‑140 (MQ=255)                  tGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                      <  1:58064/140‑1 (MQ=255)                  tGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                      >  1:174947/1‑140 (MQ=255)                      tttCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTa                                                                                                                  <  1:929325/140‑1 (MQ=255)                      tttCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTa                                                                                                                  >  2:634624/1‑140 (MQ=255)                      tttCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTa                                                                                                                  <  1:1360181/140‑1 (MQ=255)                       ttCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                      >  2:1231573/1‑135 (MQ=255)                       ttCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                      <  1:1231573/135‑1 (MQ=255)                        tCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                      >  1:372779/1‑134 (MQ=255)                        tCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                      <  2:372779/134‑1 (MQ=255)                                         cTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACaaaa                                                                                               <  2:174947/140‑1 (MQ=255)                                         cTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACaaaa                                                                                               <  2:426560/140‑1 (MQ=255)                                              aCGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAatcatc                                                                                          <  2:411862/140‑1 (MQ=255)                                                 cAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCt                                                                                       <  1:634624/140‑1 (MQ=255)                                                     aCAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTAtct                                                                                   <  1:1363110/140‑1 (MQ=255)                                                     aCAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTAtct                                                                                   <  2:1162051/140‑1 (MQ=255)                                                      cAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTAtctc                                                                                  >  2:924317/1‑140 (MQ=255)                                                         aaaaGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTctgc                                                                               <  2:637296/140‑1 (MQ=255)                                                           aaGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTctgctg                                                                             <  1:287848/140‑1 (MQ=255)                                                             gagaTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGAt                                                                           <  1:643837/140‑1 (MQ=255)                                                              agaTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATg                                                                          >  2:382698/1‑140 (MQ=255)                                                              agaTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATg                                                                          >  1:115966/1‑140 (MQ=255)                                                              agaTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATg                                                                          >  2:309110/1‑140 (MQ=255)                                                              agaTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTCACCGTATTCTCAGAGATTGACAAAatcatc                                                                                          <  2:1323409/124‑1 (MQ=255)                                                              agaTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTCACCGTATTCTCAGAGATTGACAAAatcatc                                                                                          >  1:1323409/1‑124 (MQ=255)                                                                   aaaTTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTc                                                                     >  2:980500/1‑140 (MQ=255)                                                                               tAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTc                                                         >  1:1031963/1‑140 (MQ=255)                                                                                    gCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCt                                                    >  1:1020363/1‑140 (MQ=255)                                                                                    gCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCt                                                    <  1:924317/140‑1 (MQ=255)                                                                                     ccGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTc                                                   >  1:1324774/1‑140 (MQ=255)                                                                                        cgTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGa                                                >  2:651544/1‑140 (MQ=255)                                                                                         gTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGaa                                               >  1:131400/1‑140 (MQ=255)                                                                                         gTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGaa                                               >  2:309194/1‑140 (MQ=255)                                                                                                   aCCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTc                                     >  1:676467/1‑140 (MQ=255)                                                                                                            ttATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCt                            <  2:1324774/140‑1 (MQ=255)                                                                                                                  ggTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACTGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAAtt                      >  1:602645/1‑140 (MQ=255)                                                                                                                  ggTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAAtt                      <  1:651544/140‑1 (MQ=255)                                                                                                                  ggTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAAtt                      >  2:812785/1‑140 (MQ=255)                                                                                                                    tGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAAtt                      >  2:458748/1‑138 (MQ=255)                                                                                                                    tGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAAtt                      <  1:458748/138‑1 (MQ=255)                                                                                                                    tGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCa                    >  1:522753/1‑140 (MQ=255)                                                                                                                       aTTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCAtct                 <  2:1020363/140‑1 (MQ=255)                                                                                                                         tAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCTCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATctct               >  2:1229212/1‑140 (MQ=255)                                                                                                                              tttttCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGt          <  1:1206160/140‑1 (MQ=255)                                                                                                                              tttttCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGt          <  1:87539/140‑1 (MQ=255)                                                                                                                              tttttCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGt          <  2:826745/140‑1 (MQ=255)                                                                                                                                 ttCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGtcat       <  2:115966/140‑1 (MQ=255)                                                                                                                                 ttCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGtcat       <  1:812785/140‑1 (MQ=255)                                                                                                                                     gCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACt   >  2:887231/1‑140 (MQ=255)                                                                                                                                     gCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACt   >  1:405970/1‑140 (MQ=255)                                                                                                                                      cATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTa  >  2:78028/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        AAAACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTA  >  NC_000913/1996046‑1996318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑