Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,026,736	G→A	H428Y (CAT→TAT)	dgcQ ←	putative diguanylate cyclase DgcQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,026,736	0	G	A	100.0%	356.7 / NA	100	H428Y (CAT→TAT)	dgcQ	putative diguanylate cyclase DgcQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (51/49);  total (51/49)

GGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAA  >  NC_000913/2026599‑2026867                                                                                                                                          |                                                                                                                                    ggCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATAtt                                                                                                                                   >  2:977620/1‑140 (MQ=255)   cAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTgg                                                                                                                                 >  1:238190/1‑140 (MQ=255)    aaCGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGt                                                                                                                                <  1:1199501/140‑1 (MQ=255)    aaCGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGt                                                                                                                                <  2:238190/140‑1 (MQ=255)        tCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGt                                                                                                                            >  2:1272748/1‑140 (MQ=255)         ccTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTc                                                                                                                           <  1:66171/140‑1 (MQ=255)            gcgcACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGa                                                                                                                        <  2:581228/140‑1 (MQ=255)             cgcacgcaAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGac                                                                                                                       <  1:318412/140‑1 (MQ=255)                 cgcaAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACaa                                                                                                                   >  1:1072415/1‑140 (MQ=255)                 cgcaAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACaa                                                                                                                   <  1:553187/140‑1 (MQ=255)                 cgcaAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATAACCAAAGCGGTCATTAATCGCTTTAAAAGGGTCAAGATCAACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACaa                                                                                                                   >  2:596407/1‑140 (MQ=255)                  gcaAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAAt                                                                                                                  >  1:364462/1‑140 (MQ=255)                  gcaAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAAt                                                                                                                  >  1:403402/1‑140 (MQ=255)                     aGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTa                                                                                                               >  2:176367/1‑140 (MQ=255)                       gAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTagc                                                                                                             >  1:1102132/1‑140 (MQ=255)                       gAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTagc                                                                                                             >  1:473801/1‑140 (MQ=255)                         aCTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTagcga                                                                                                           <  1:218879/140‑1 (MQ=255)                            gCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTagcgagcg                                                                                                        >  2:16838/1‑140 (MQ=255)                            gCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTagcgagcg                                                                                                        >  2:490625/1‑140 (MQ=255)                            gCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTagcgagcg                                                                                                        >  1:93880/1‑140 (MQ=255)                            gCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTagcgagcg                                                                                                        >  1:1304857/1‑140 (MQ=255)                            gCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTagcgagcg                                                                                                        >  1:695326/1‑140 (MQ=255)                                  taatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACggg                                                                                                  <  1:675290/140‑1 (MQ=255)                                  taatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACggg                                                                                                  <  1:309802/140‑1 (MQ=255)                                  taatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACggg                                                                                                  <  1:668918/140‑1 (MQ=255)                                  taatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACggg                                                                                                  <  2:295122/140‑1 (MQ=255)                                  taatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACggg                                                                                                  <  1:1323920/140‑1 (MQ=255)                                  taatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACggg                                                                                                  <  2:1227816/140‑1 (MQ=255)                                  taatCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACggg                                                                                                  <  2:448283/140‑1 (MQ=255)                                     tCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCtt                                                                                               >  1:90693/1‑140 (MQ=255)                                          cagcaTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCg                                                                                          >  2:1158853/1‑140 (MQ=255)                                          cagcaTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCg                                                                                          >  1:1138589/1‑140 (MQ=255)                                          cagcaTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCg                                                                                          >  2:305694/1‑140 (MQ=255)                                            gcaTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGaa                                                                                        <  2:533119/140‑1 (MQ=255)                                             caTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAAc                                                                                       >  2:115817/1‑140 (MQ=255)                                              aTGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACa                                                                                      <  2:695326/140‑1 (MQ=255)                                               tGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAg                                                                                     <  2:93880/140‑1 (MQ=255)                                               tGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAg                                                                                     >  2:1015937/1‑140 (MQ=255)                                               tGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAg                                                                                     <  2:90693/140‑1 (MQ=255)                                                 agaaagaaCACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTg                                                                                   >  1:379932/1‑140 (MQ=255)                                                    aagaaCACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGTGAGCGGACGGGCTTTTTCGAACAGTgcgc                                                                                <  1:865269/140‑1 (MQ=255)                                                    aagaaCACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTgcgc                                                                                <  2:379932/140‑1 (MQ=255)                                                    aagaaCACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTgcgc                                                                                <  2:1072415/140‑1 (MQ=255)                                                     agaaCACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGcc                                                                               <  2:1138589/140‑1 (MQ=255)                                                      gaaCACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCa                                                                              <  2:364462/140‑1 (MQ=255)                                                      gaaCACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCa                                                                              <  1:490625/140‑1 (MQ=255)                                                      gaaCACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCa                                                                              <  2:837021/140‑1 (MQ=255)                                                      gaaCACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCa                                                                              <  2:162543/140‑1 (MQ=255)                                                       aaCACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGcc                                                                               <  2:69248/138‑1 (MQ=255)                                                       aaCACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGcc                                                                               >  1:69248/1‑138 (MQ=255)                                                         cacGGTCGCCCGCCTGATGACCGAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACgg                                                                           >  1:63206/1‑140 (MQ=255)                                                         cacGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTg                                                                                   >  2:1059189/1‑132 (MQ=255)                                                         cacGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTg                                                                                   <  1:1059189/132‑1 (MQ=255)                                                         cacGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACgg                                                                           <  1:780995/140‑1 (MQ=255)                                                            ggTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGcc                                                                               >  1:274844/1‑133 (MQ=255)                                                            ggTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGcc                                                                               <  2:274844/133‑1 (MQ=255)                                                             gTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTtat                                                                       >  1:1324827/1‑140 (MQ=255)                                                              tCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTtata                                                                      >  2:826003/1‑140 (MQ=255)                                                                 cccgccTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATaa                                                                   <  1:1012039/140‑1 (MQ=255)                                                                     ccTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAAcgc                                                               <  1:115817/140‑1 (MQ=255)                                                                      cTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAAcgcg                                                              >  2:673148/1‑140 (MQ=255)                                                                       tgatgaCCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGt                                                             <  1:521842/140‑1 (MQ=255)                                                                        gatgaCCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGtt                                                            >  1:930334/1‑140 (MQ=255)                                                                                aaGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATaa                                                                   <  2:1007351/125‑1 (MQ=255)                                                                                aaGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATaa                                                                   >  1:1007351/1‑125 (MQ=255)                                                                                aaGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTc                                                    >  2:630380/1‑140 (MQ=255)                                                                                  gCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGt                                                  >  2:1184913/1‑140 (MQ=255)                                                                                     gTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGcc                                               >  1:1296447/1‑140 (MQ=255)                                                                                           aaTCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCt                                         >  2:935410/1‑140 (MQ=255)                                                                                            aTCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTg                                        <  1:579649/140‑1 (MQ=255)                                                                                             tCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGc                                       >  1:1120998/1‑140 (MQ=255)                                                                                             tCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGc                                       >  1:1089833/1‑140 (MQ=255)                                                                                              cGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGcc                                      >  1:1104204/1‑140 (MQ=255)                                                                                               gCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCa                                     >  1:318122/1‑140 (MQ=255)                                                                                                cTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCAc                                    >  1:220488/1‑140 (MQ=255)                                                                                                    aaaaTGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCa                                <  1:234808/140‑1 (MQ=255)                                                                                                      aaTGGTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAAc                              <  1:1158853/140‑1 (MQ=255)                                                                                                         ggTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAg                           <  2:403402/140‑1 (MQ=255)                                                                                                          gTCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGc                          >  1:453656/1‑140 (MQ=255)                                                                                                           tCAAGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCt                         >  2:209911/1‑140 (MQ=255)                                                                                                             aaGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCttt                       <  2:1120998/140‑1 (MQ=255)                                                                                                             aaGATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCttt                       >  2:525848/1‑140 (MQ=255)                                                                                                               gATCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGc                     <  2:473801/140‑1 (MQ=255)                                                                                                                 tCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAg                   <  1:630380/140‑1 (MQ=255)                                                                                                                 tCGACCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAg                   <  2:318122/140‑1 (MQ=255)                                                                                                                    aCCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAac                <  1:1015937/140‑1 (MQ=255)                                                                                                                    aCCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAac                >  2:693305/1‑140 (MQ=255)                                                                                                                    aCCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAac                >  1:848367/1‑140 (MQ=255)                                                                                                                    aCCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAac                >  1:189653/1‑140 (MQ=255)                                                                                                                    aCCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAac                >  1:1290162/1‑140 (MQ=255)                                                                                                                    aCCTGAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAac                >  1:1099722/1‑140 (MQ=255)                                                                                                                        gAATGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAacatac            <  1:16838/140‑1 (MQ=255)                                                                                                                         aaTGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAacataca           <  1:176367/140‑1 (MQ=255)                                                                                                                         aaTGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAacataca           <  2:1102132/140‑1 (MQ=255)                                                                                                                          aTGACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAacatacat          >  1:1314249/1‑140 (MQ=255)                                                                                                                            gACAGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGt        <  2:63206/140‑1 (MQ=255)                                                                                                                               aGAAAAAGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGc     >  2:464254/1‑140 (MQ=255)                                                                                                                                 aaaaaGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTa   <  2:350352/140‑1 (MQ=255)                                                                                                                                 aaaaaGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTa   >  2:438181/1‑140 (MQ=255)                                                                                                                                  aaaaGGATATTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTaa  <  2:220488/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    GGCAACGTCCTGCGCACGCAAGGAACTGCTAATTAATCCGGCAGCATGAGAAAGAACACGGTCGCCCGCCTGATGACCAAAGCGGTCATTAATCGCTTTAAAATGGTCAAGATCGACCTGAATGACAGAAAAAGGATGTTGGTGCGTCTGACACAATTTAGCGAGCGGACGGGCTTTTTCGAACAGTGCGCCACGGTTATATAAACGCGTTAAGGTGTCGTGCCACGCCTGCCACTGCAACGAGCTTTGCAGAACATACATGTTGCTAA  >  NC_000913/2026599‑2026867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑