Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,217,529	G→A	F343F (TTC→TTT)	yehY ←	glycine betaine ABC transporter membrane subunit YehY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,217,529	0	G	A	100.0%	81.5 / NA	24	F343F (TTC→TTT)	yehY	glycine betaine ABC transporter membrane subunit YehY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/4);  total (20/4)

TAAATTCAATCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGTAACTGAACATGCAGGAAACGC  >  NC_000913/2217395‑2217664                                                                                                                                       |                                                                                                                                        tAAATTCAATCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAAc                                                                                                                                    >  1:527317/1‑140 (MQ=255)    aTTCAATCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGGCCCTGAAAAACCAg                                                                                                                                 <  1:1171463/140‑1 (MQ=255)          tCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCAcc                                                                                                                           >  2:721364/1‑140 (MQ=255)          tCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCAcc                                                                                                                           >  1:918028/1‑140 (MQ=255)                 tttcccccTCCAGCGGTGCGAAAAGAAAATCAAACAGCGCGTCGATAAGCACCGCCAGAAAAATTACCGGGATCACCCCCAGCAACACAAAAACAATGGCGCTGCTTAGCAGCCCCTAAAAAACCAGCGCCCCAAAACCg                                                                                                                    <  2:319517/134‑1 (MQ=255)                       ccTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCCCCGCCAGAACAATTACCGGGATCCCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCACCAAAACCGCCTgcg                                                                                                              <  1:1144316/140‑1 (MQ=255)                       acagcagcagTGCAAAAAGCAAATCGAACAGCGCGTGGGTAAGCCCCGCCAGAACAATTCCCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCACCAAAACCGCCTgcg                                                                                                              <  2:163787/136‑1 (MQ=255)                                  gCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCCCCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAAcgc                                                                                                   <  1:721364/140‑1 (MQ=255)                                  gCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAAcgc                                                                                                   >  2:36620/1‑140 (MQ=255)                                  gCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAAcgc                                                                                                   >  1:686334/1‑140 (MQ=255)                                      tAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAAcgcctgt                                                                                               >  1:163787/1‑137 (MQ=255)                                           aaaTCGAACAGCGCGTCGGTAAGCACTGCCAGAACAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCAc                                                                                          >  1:464998/1‑140 (MQ=255)                                             aTCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCg                                                                                        >  2:743219/1‑140 (MQ=255)                                                       gcgTCGGTAAGCACCGCCAGAACAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTTAAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTAc                                                                              >  1:637856/1‑140 (MQ=255)                                                        cgTCGGTAAGCACCGCCAGAACAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACa                                                                             >  1:10923/1‑140 (MQ=255)                                                                   aCCGCCAGAACAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCAt                                                                  >  2:972791/1‑140 (MQ=255)                                                                     cGCCAGAACAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATca                                                                >  1:669392/1‑140 (MQ=255)                                                                       ccAGAACAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCACCAAAACCGCCTGCGCCGATGAACGCCGCAACCACCGCCATACCTACAGTTTGCACCATcacc                                                              >  2:1304894/1‑140 (MQ=255)                                                                           aaCAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCAccc                                                          >  2:1212781/1‑140 (MQ=255)                                                                            aCAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCg                                                         >  1:562329/1‑140 (MQ=255)                                                                                     gggATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTgcg                                                >  1:1081561/1‑140 (MQ=255)                                                                                            cccccAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAAt                                         >  1:357338/1‑140 (MQ=255)                                                                                                  gCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGt                                   >  1:450116/1‑140 (MQ=255)                                                                                                                 tGGCGCTGCTTAGCAGCCCCTGAAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGTAAc                    >  1:857832/1‑140 (MQ=255)                                                                                                                                   ccTGAAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGTAACTGAACATGCAGGAAACGc  >  1:155748/1‑140 (MQ=255)                                                                                                                                   ccTGAAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGTAACTGAACATGCAGGAAACGc  >  2:873403/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        TAAATTCAATCATTACGTTTCACCTTCAGCAGTGCGATAAGCAAATCGAACAGCGCGTCGGTAAGCACCGCCAGAACAATTACCGGGATCACCCCCAGCAACACTAAATCAATGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGTAACTGAACATGCAGGAAACGC  >  NC_000913/2217395‑2217664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑