Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,343,512	G→A	P220S (CCT→TCT)	yfaL ←	putative autotransporter adhesin YfaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,343,512	0	G	A	100.0%	305.0 / NA	86	P220S (CCT→TCT)	yfaL	putative autotransporter adhesin YfaL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (48/38);  total (48/38)

GTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCATTATTAATGGTATAAATT  >  NC_000913/2343375‑2343648                                                                                                                                          |                                                                                                                                         gTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAAt                                                                                                                                        >  2:701536/1‑140 (MQ=255)                     caACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCtattatta                                                                                                                    >  2:1322183/1‑140 (MQ=255)                        aaaaaaTAAAAAAAAGATGGGGAGCCGTCGCGTTTTTGATATTTCCCCCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCg                                                                                                                 <  2:942232/137‑1 (MQ=255)                                       gaggggggGCCTTCTCGTTTTTGTTATATCCCCCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGcc                                                                                                  <  2:319711/136‑1 (MQ=255)                                          aGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCcg                                                                                               >  2:568762/1‑140 (MQ=255)                                            ggAGCCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCcgcc                                                                                             >  1:502825/1‑140 (MQ=255)                                            ggAGCCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCcgcc                                                                                             >  1:434378/1‑140 (MQ=255)                                            ggAGCCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCcgcc                                                                                             >  1:319711/1‑140 (MQ=255)                                             gAGCCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCcgcca                                                                                            >  1:551550/1‑140 (MQ=255)                                              aGCCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCcgccat                                                                                           >  2:1310327/1‑140 (MQ=255)                                               gCCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCcgccatc                                                                                          >  2:806859/1‑140 (MQ=255)                                                ccGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCcgccatcg                                                                                         >  1:16403/1‑140 (MQ=255)                                                 cGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCcgccatcgc                                                                                        >  1:622162/1‑140 (MQ=255)                                                           ttGTTATATCCCCCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTa                                                                              <  1:479349/140‑1 (MQ=255)                                                              gTATATATCCCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACt                                                                           <  1:1322183/140‑1 (MQ=255)                                                                 tatccccccccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACttgt                                                                        <  2:24770/133‑1 (MQ=255)                                                                  atatCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACttgtt                                                                       >  1:852881/1‑140 (MQ=255)                                                                   tccccccccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACttgttg                                                                      <  2:551550/135‑1 (MQ=255)                                                                   tccccccccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACttgttg                                                                      <  2:1135863/135‑1 (MQ=255)                                                                   tccccccccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCAATCGCCATCACTGTAACttgttg                                                                      <  2:478626/135‑1 (MQ=255)                                                                   tatcctcccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACttgttg                                                                      <  2:1154051/134‑1 (MQ=255)                                                                   tatCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACttgttg                                                                      >  1:694322/1‑140 (MQ=255)                                                                   tatCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACttgttg                                                                      <  1:568762/140‑1 (MQ=255)                                                                   tatCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACctgtct                                                                      >  1:328459/1‑138 (MQ=255)                                                                   tacccccccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACttgttg                                                                      <  2:1132028/135‑1 (MQ=255)                                                                   tacccccccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACttgttg                                                                      <  2:272045/135‑1 (MQ=255)                                                                     tCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGAt                                                                    >  2:1124654/1‑140 (MQ=255)                                                                     tCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGAt                                                                    >  2:258884/1‑140 (MQ=255)                                                                     tCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGAt                                                                    >  2:428010/1‑140 (MQ=255)                                                                      cGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATg                                                                   >  2:946838/1‑140 (MQ=255)                                                                        ccccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACttgttg                                                                      <  2:44422/135‑1 (MQ=255)                                                                        ccccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACttgttg                                                                      >  1:44422/1‑135 (MQ=255)                                                                        ccccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGta                                                                 >  1:349011/1‑140 (MQ=255)                                                                        ccccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGta                                                                 >  1:582557/1‑140 (MQ=255)                                                                        ccccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGta                                                                 <  2:694322/140‑1 (MQ=255)                                                                        ccccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGta                                                                 >  1:696897/1‑140 (MQ=255)                                                                        ccccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGta                                                                 >  2:1016411/1‑140 (MQ=255)                                                                        ccccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGta                                                                 >  1:731678/1‑140 (MQ=255)                                                                         cccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGtat                                                                <  2:1226699/140‑1 (MQ=255)                                                                         cccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGtat                                                                >  2:308037/1‑140 (MQ=255)                                                                         cccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGtat                                                                <  2:502825/140‑1 (MQ=255)                                                                         cccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGtat                                                                <  1:1124654/140‑1 (MQ=255)                                                                         cccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGtat                                                                >  1:1133131/1‑140 (MQ=255)                                                                         cccGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGtat                                                                <  2:16403/140‑1 (MQ=255)                                                                             ccATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACttgttg                                                                      <  1:1044753/130‑1 (MQ=255)                                                                             ccATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACttgttg                                                                      >  2:1044753/1‑130 (MQ=255)                                                                             ccATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGc                                                            >  1:349896/1‑140 (MQ=255)                                                                                    cTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGgttg                                                     <  2:396247/140‑1 (MQ=255)                                                                                       cGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTa                                                  >  2:557201/1‑140 (MQ=255)                                                                                       cGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTa                                                  >  1:699826/1‑140 (MQ=255)                                                                                         gCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATc                                                <  1:1016411/140‑1 (MQ=255)                                                                                         gCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATc                                                <  1:308037/140‑1 (MQ=255)                                                                                             tgtTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCaaaa                                            >  2:573858/1‑140 (MQ=255)                                                                                             tgtTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCaaaa                                            >  1:565740/1‑140 (MQ=255)                                                                                              gtTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCaaaaa                                           <  2:347221/140‑1 (MQ=255)                                                                                               ttATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAAt                                          <  2:1133131/140‑1 (MQ=255)                                                                                               ttATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAAt                                          >  1:353408/1‑140 (MQ=255)                                                                                                    tGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACAt                                     <  1:486448/140‑1 (MQ=255)                                                                                                     gTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATc                                    <  1:946838/140‑1 (MQ=255)                                                                                                       aaaGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCAc                                  <  1:1193843/140‑1 (MQ=255)                                                                                                        aaGGCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACt                                 <  2:696897/140‑1 (MQ=255)                                                                                                           gCAGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTtaa                              >  2:1151680/1‑140 (MQ=255)                                                                                                             aGTGTTATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTtaaat                            >  1:1249629/1‑140 (MQ=255)                                                                                                                  tATTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACg                       <  2:852881/140‑1 (MQ=255)                                                                                                                   aTTTACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCCCTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGt                      <  2:1249629/140‑1 (MQ=255)                                                                                                                     ttACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCa                    >  1:1291929/1‑140 (MQ=255)                                                                                                                     ttACTATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCa                    >  1:851742/1‑140 (MQ=255)                                                                                                                         tATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAATATCACTtaaata                           <  1:1069771/129‑1 (MQ=255)                                                                                                                         tATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAATATCACTtaaata                           >  2:1069771/1‑129 (MQ=255)                                                                                                                         tATCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCattat                >  1:856258/1‑140 (MQ=255)                                                                                                                           tCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCAttatta              <  1:806859/140‑1 (MQ=255)                                                                                                                           tCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCAttatta              <  1:701536/140‑1 (MQ=255)                                                                                                                           tCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCAttatta              <  1:573858/140‑1 (MQ=255)                                                                                                                           tCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCAttatta              >  1:390318/1‑140 (MQ=255)                                                                                                                           tCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCAttatta              >  2:708556/1‑140 (MQ=255)                                                                                                                           tCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCAttatta              <  2:851742/140‑1 (MQ=255)                                                                                                                           tCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCAttatta              >  1:1375421/1‑140 (MQ=255)                                                                                                                           tCGTATAACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCAttatta              <  1:1205060/140‑1 (MQ=255)                                                                                                                              tataACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCATTATTAATg           >  1:989776/1‑140 (MQ=255)                                                                                                                              tataACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCATTATTAATg           >  1:617605/1‑140 (MQ=255)                                                                                                                              tataACCTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCATTATTAATg           >  1:393230/1‑140 (MQ=255)                                                                                                                                    cTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCATTATTAATGGTATaa     <  1:428010/140‑1 (MQ=255)                                                                                                                                    cTGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCATTATTAATGGTATaa     <  1:258884/140‑1 (MQ=255)                                                                                                                                     tGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCATTATTAATGGTATaaa    >  2:280720/1‑140 (MQ=255)                                                                                                                                     tGAAGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCATTATTAATGGTATaaa    >  2:21813/1‑140 (MQ=255)                                                                                                                                       aaGAATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCATTATTAATGGTATAAAtt  >  1:1040710/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         GTTCTGGCTGTAGCTGTCATCAACAGAAATATCAATAAGATAGGGAGCCGTCACGCTATTGGTATATATCGCCCCGCCATAACCTTCGGCAGTGTTATTTGTAAAGGCAGTGTTATTTACTATCGTATAACCTGAAGGATGCTTGCTGTCGCTATTATTATCGGTAACATCGATTGCCCCGCCATCGCCATCACTGTAACTTGTTGATGTATATGCCTGGTTGTTATCAAAAATAACATCACTTAAATAAACGTCATTATTAATGGTATAAATT  >  NC_000913/2343375‑2343648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑