Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,378,869	T→C	T131A (ACG→GCG)	menD ←	2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,378,869	0	T	C	100.0%	266.0 / NA	73	T131A (ACG→GCG)	menD	2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/32);  total (41/32)

CATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTCTCCGGTTAACCCGGCTTCAATCAGTGCCGGATAGAGA  >  NC_000913/2378734‑2379005                                                                                                                                        |                                                                                                                                         cATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCGGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCgggg                                                                                                                                      >  1:838509/1‑140 (MQ=255)          gTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCg                                                                                                                             >  1:1219615/1‑140 (MQ=255)           ttCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGa                                                                                                                            >  2:653560/1‑140 (MQ=255)            tCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGaa                                                                                                                           <  2:1219615/140‑1 (MQ=255)             cAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAAc                                                                                                                          >  2:293652/1‑140 (MQ=255)                       gCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCt                                                                                                                <  2:63322/140‑1 (MQ=255)                            tGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGa                                                                                                           <  1:653560/140‑1 (MQ=255)                               tatGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAAtt                                                                                                        >  2:466127/1‑140 (MQ=255)                                     cTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGa                                                                                                  >  1:765518/1‑140 (MQ=255)                                     cTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGa                                                                                                  >  1:946986/1‑140 (MQ=255)                                     cTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGa                                                                                                  >  2:385592/1‑140 (MQ=255)                                     cTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGa                                                                                                  >  1:1395627/1‑140 (MQ=255)                                     cTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGa                                                                                                  >  1:261863/1‑140 (MQ=255)                                        cccccGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTc                                                                                               >  1:786486/1‑140 (MQ=255)                                        cccccGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTc                                                                                               >  2:1281879/1‑140 (MQ=255)                                         ccccGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTcg                                                                                              >  1:114597/1‑140 (MQ=255)                                           ccGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTcgcg                                                                                            <  2:946986/140‑1 (MQ=255)                                            cGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCgcgc                                                                                           <  2:1171026/140‑1 (MQ=255)                                            cGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCgcgc                                                                                           >  1:1171026/1‑140 (MQ=255)                                            cGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCgcgc                                                                                           <  1:1194228/140‑1 (MQ=255)                                              cATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGcgccg                                                                                         <  2:1206382/140‑1 (MQ=255)                                               aTGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCGGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGcgccgc                                                                                        <  2:838509/140‑1 (MQ=255)                                               aTGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGcgccgc                                                                                        <  2:377899/140‑1 (MQ=255)                                                 gAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAg                                                                                      <  1:1101325/140‑1 (MQ=255)                                                  aaGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGcgccgc                                                                                        <  1:1016535/137‑1 (MQ=255)                                                  aaGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGcgccgc                                                                                        >  2:1016535/1‑137 (MQ=255)                                                  aaGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGt                                                                                     >  1:809044/1‑140 (MQ=255)                                                       tACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTcgcg                                                                                            >  1:1359840/1‑128 (MQ=255)                                                       tACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTcgcg                                                                                            <  2:1359840/128‑1 (MQ=255)                                                       tACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAAtt                                                                                >  1:795335/1‑140 (MQ=255)                                                         ccGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAg                                                                              <  1:466127/140‑1 (MQ=255)                                                            agagCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTc                                                                           >  2:1176721/1‑140 (MQ=255)                                                             gagCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTcc                                                                          <  1:49131/140‑1 (MQ=255)                                                                cGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCcgg                                                                        >  2:224487/1‑139 (MQ=255)                                                                cGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCcgg                                                                        <  1:224487/139‑1 (MQ=255)                                                                  tGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCcggcgg                                                                     >  1:73199/1‑140 (MQ=255)                                                                  tGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCcggcgg                                                                     >  1:758684/1‑140 (MQ=255)                                                                   ggTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCggcggg                                                                    >  1:181238/1‑140 (MQ=255)                                                                    gTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCggcgggc                                                                   >  2:683778/1‑140 (MQ=255)                                                                       gATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGAt                                                                <  2:786486/140‑1 (MQ=255)                                                                          ggTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATcgg                                                             <  2:795335/140‑1 (MQ=255)                                                                             aGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATcggcgg                                                          <  1:293652/140‑1 (MQ=255)                                                                                   cAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAaga                                                    >  1:117576/1‑140 (MQ=255)                                                                                        aaCGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATc                                               <  1:797678/140‑1 (MQ=255)                                                                                             gCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTTAAAGAAACAGttt                                          >  2:1354654/1‑140 (MQ=255)                                                                                                 ggATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTtct                                      <  1:703580/140‑1 (MQ=255)                                                                                                      tCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTTTcccgg                                 >  2:590443/1‑137 (MQ=255)                                                                                                          gggTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTCTCCGGTTAAc                             <  1:1354654/140‑1 (MQ=255)                                                                                                           ggTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTCTCCGGTTAAcc                            <  1:1176721/140‑1 (MQ=255)                                                                                                           ggTCGGGCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTCTCCGGTTAAcc                            <  2:200119/140‑1 (MQ=255)                                                                                                                ggCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTTAGAGGAACAGGTTTTTTCCGGGTAACCCGGcc                       >  2:467616/1‑139 (MQ=255)                                                                                                                ggCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTTTTCCGTTAAACCGGcc                       >  2:1108387/1‑139 (MQ=255)                                                                                                                ggCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTCTCCGGTTAACCCGGCt                       >  1:380125/1‑140 (MQ=255)                                                                                                                ggCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTCTCCGGTTAACCCGGCt                       >  1:1100788/1‑140 (MQ=255)                                                                                                                ggCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTCTCCGGTTAACCCGGCt                       >  1:358581/1‑140 (MQ=255)                                                                                                                ggCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTCTCCGGTTAACCCGGCt                       >  1:557663/1‑140 (MQ=255)                                                                                                                ggCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTCTCCGGTTAACCCGGCt                       <  1:467616/140‑1 (MQ=255)                                                                                                                ggCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAAACAGTTTTTTTCCGGTTAACCCGGCt                       >  1:377197/1‑140 (MQ=255)                                                                                                                ggCGCGGCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGGTAACGGAATCAGGTTTTCTCCGGGTAAACCGGcc                       >  2:77461/1‑139 (MQ=255)                                                                                                                     ggCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTCTCCGGTTAACCCGGCTTCAAt                  <  2:1100788/140‑1 (MQ=255)                                                                                                                     ggCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTCTCCGGTTAACCCGGCTTCAAt                  <  1:385592/140‑1 (MQ=255)                                                                                                                     ggCAATGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTCTCCGGTTAACCCGGCTTCAAt                  <  2:117576/140‑1 (MQ=255)                                                                                                                          tGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTCTCCGGTTAACCCGGCTTCAATCAGTg             <  1:248963/140‑1 (MQ=255)                                                                                                                          tGAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTCTCCGGTTAACCCGGCTTCAATCAGTg             <  1:683778/140‑1 (MQ=255)                                                                                                                           gAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTCTCCGGTTAACCCGGCTTCAATCAGTGc            <  1:1352381/140‑1 (MQ=255)                                                                                                                           gAAATACTGTGCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTCTCCGGTTAACCCGGCTTCAATCAGTGc            <  1:394556/140‑1 (MQ=255)                                                                                                                                  tgtgCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTCTCCGGTTAACCCGGCTTCAATCAGTGCCGGATag     <  2:809044/140‑1 (MQ=255)                                                                                                                                   gtgCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTTAGAGAATCAGGTTTTTTTCGGTTTACCCCGCTTCTATCCGTGCCCGAAagg    >  2:248963/1‑139 (MQ=255)                                                                                                                                    tgCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTTTCCGGTTAACCCCGCTTCAATCAGTGCCGGATagag   >  1:1330229/1‑140 (MQ=255)                                                                                                                                    tgCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTTTCCGGGTAAACCGGCTTCCATCAGTGCCGGGTagag   >  1:981161/1‑140 (MQ=255)                                                                                                                                    tgCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAAACAGTTTTTTTCCCGGTAACCCGGCTTCAATCAATGCCGGGTagag   >  1:1259565/1‑140 (MQ=255)                                                                                                                                    tgCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAAAGAAACAATTTTTTTTTCGCTAACCCCGCCTCAATCAACGCCCGAtgggg   >  2:696764/1‑136 (MQ=255)                                                                                                                                    tgCGCGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGGTTAGAGAATCAATTTTTTTTCGGTTAACCCCGGGTCAATCAGTGGCGGGTaaag   >  2:1036082/1‑140 (MQ=255)                                                                                                                                     gcgcGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAAACAGGTTTTCTCCCGCTCACCCCGCTTCAATCAGTGTCGGGTAgaga  >  2:1083793/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         CATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAATCAGTTTTTCTCCGGTTAACCCGGCTTCAATCAGTGCCGGATAGAGA  >  NC_000913/2378734‑2379005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑