Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,860,452	T→C	intergenic (+36/+15)	pphB → / ← ygbI	phosphoprotein phosphatase 2/putative DeoR‑type DNA‑binding transcriptional regulator YgbI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,860,452	0	T	C	100.0%	307.3 / NA	84	intergenic (+36/+15)	pphB/ygbI	phosphoprotein phosphatase 2/putative DeoR‑type DNA‑binding transcriptional regulator YgbI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/40);  total (44/40)

GGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCAT  >  NC_000913/2860315‑2860587                                                                                                                                          |                                                                                                                                        ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGgcgt                                                                                                                                       >  2:1169875/1‑140 (MQ=255) ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGgcgt                                                                                                                                       >  2:721153/1‑140 (MQ=255) ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGgcgt                                                                                                                                       >  2:1181578/1‑140 (MQ=255)  gACATATGATGTTTGATAACATTCGGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGtt                                                                                                                                      >  1:777003/1‑140 (MQ=255)      tatGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTg                                                                                                                                  >  2:892956/1‑140 (MQ=255)      tatGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTg                                                                                                                                  >  2:613735/1‑140 (MQ=255)       atgatgTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGc                                                                                                                                 >  1:522132/1‑140 (MQ=255)           tgTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGc                                                                                                                             <  1:166568/140‑1 (MQ=255)             tttGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCa                                                                                                                           <  1:1397950/140‑1 (MQ=255)               tGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCatt                                                                                                                         >  2:1014703/1‑140 (MQ=255)                    aCATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCa                                                                                                                    <  1:242724/140‑1 (MQ=255)                     cATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCAt                                                                                                                   <  2:1131624/140‑1 (MQ=255)                     cATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCAt                                                                                                                   <  2:522132/140‑1 (MQ=255)                     cATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCAt                                                                                                                   >  1:1297285/1‑140 (MQ=255)                     cATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCAt                                                                                                                   >  1:699735/1‑140 (MQ=255)                     cATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCAt                                                                                                                   >  2:66429/1‑140 (MQ=255)                      aTTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCattatt                                                                                                                      <  1:155103/136‑1 (MQ=255)                      aTTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCattatt                                                                                                                      >  2:155103/1‑136 (MQ=255)                        tCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCAtttt                                                                                                                >  1:764835/1‑140 (MQ=255)                            aCGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGc                                                                                                            <  1:539248/140‑1 (MQ=255)                               ttCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTAc                                                                                                         >  1:774910/1‑140 (MQ=255)                               ttCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTAc                                                                                                         >  2:301444/1‑140 (MQ=255)                                tCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTAcc                                                                                                        <  2:137170/140‑1 (MQ=255)                                 cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCa                                                                                                       >  1:590775/1‑140 (MQ=255)                                 cGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCa                                                                                                       >  2:1065796/1‑140 (MQ=255)                                    tAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCa                                                                                                    <  2:526824/140‑1 (MQ=255)                                        cAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAaga                                                                                                >  1:954498/1‑140 (MQ=255)                                           aTTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAagagag                                                                                             >  1:777964/1‑140 (MQ=255)                                             ttATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGAt                                                                                           <  1:1093592/140‑1 (MQ=255)                                             ttATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGAt                                                                                           <  2:954498/140‑1 (MQ=255)                                             ttATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGAt                                                                                           <  2:341940/140‑1 (MQ=255)                                               atatTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATcc                                                                                         <  1:613735/140‑1 (MQ=255)                                                     aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                                                                   >  2:741287/1‑140 (MQ=255)                                                     aTACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTc                                                                                   >  1:783303/1‑140 (MQ=255)                                                       aCCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCAc                                                                                 <  1:301444/140‑1 (MQ=255)                                                       aCCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCAc                                                                                 <  2:1297285/140‑1 (MQ=255)                                                          ggATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAg                                                                              >  1:642540/1‑140 (MQ=255)                                                            aTCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcg                                                                            >  1:169623/1‑140 (MQ=255)                                                            aTCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcg                                                                            <  1:11205/140‑1 (MQ=255)                                                            aTCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcg                                                                            <  2:666315/140‑1 (MQ=255)                                                             tCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTACCCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcgc                                                                           <  2:217565/140‑1 (MQ=255)                                                             tCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcgc                                                                           <  2:764835/140‑1 (MQ=255)                                                               gCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcgcgc                                                                         >  1:792490/1‑140 (MQ=255)                                                               gCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAgcgcgc                                                                         >  2:974021/1‑140 (MQ=255)                                                                ccGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGcgcgcg                                                                        <  1:741287/140‑1 (MQ=255)                                                                       gcgggcggCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCa                                                                 <  1:1014703/140‑1 (MQ=255)                                                                       gcgggcggCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCa                                                                 <  1:230250/140‑1 (MQ=255)                                                                        cgggcggCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCAc                                                                >  1:960139/1‑140 (MQ=255)                                                                        cgggcggCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCAc                                                                >  1:1211547/1‑140 (MQ=255)                                                                              gcTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCg                                                          <  1:1221649/140‑1 (MQ=255)                                                                                tGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGc                                                        <  1:721153/140‑1 (MQ=255)                                                                                      tttATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcg                                                  <  2:642540/140‑1 (MQ=255)                                                                                      tttATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcg                                                  <  1:879054/140‑1 (MQ=255)                                                                                         ataaaataaaGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcgtcg                                               >  1:731475/1‑140 (MQ=255)                                                                                          taaaataaaGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcgtcgt                                              <  1:892956/140‑1 (MQ=255)                                                                                          taaaataaaGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACcgtcgt                                              <  1:1181578/140‑1 (MQ=255)                                                                                               taaaGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGta                                         >  2:836533/1‑140 (MQ=255)                                                                                                  agtagtCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGtaata                                      >  2:843852/1‑140 (MQ=255)                                                                                                   gtagtCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGtaataa                                     <  2:774910/140‑1 (MQ=255)                                                                                                       tctcATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTg                                 >  2:224537/1‑140 (MQ=255)                                                                                                       tctcATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTg                                 >  2:707828/1‑140 (MQ=255)                                                                                                       tctcATGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTg                                 >  1:1068373/1‑140 (MQ=255)                                                                                                           aTGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCa                             >  2:59736/1‑140 (MQ=255)                                                                                                            tGCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCaa                            >  2:104091/1‑140 (MQ=255)                                                                                                             gCTTCTTCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCaaa                           <  1:1065796/140‑1 (MQ=255)                                                                                                              cttcttCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAAc                          >  1:254559/1‑140 (MQ=255)                                                                                                              cttcttCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAAc                          >  2:224553/1‑140 (MQ=255)                                                                                                              cttcttCTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAAc                          <  2:207200/140‑1 (MQ=255)                                                                                                               ttcttcTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACt                         <  1:59736/140‑1 (MQ=255)                                                                                                               ttcttcTGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACt                         <  2:777964/140‑1 (MQ=255)                                                                                                                 cttctGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCg                       >  2:453216/1‑140 (MQ=255)                                                                                                                 cttctGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCg                       >  1:681059/1‑140 (MQ=255)                                                                                                                   tctGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCt                     >  1:446079/1‑140 (MQ=255)                                                                                                                   tctGTGAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCt                     <  1:836533/140‑1 (MQ=255)                                                                                                                       tgAAGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAAt                 >  2:981590/1‑140 (MQ=255)                                                                                                                         aaGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACt                         <  2:1106634/130‑1 (MQ=255)                                                                                                                         aaGCATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACt                         >  1:1106634/1‑130 (MQ=255)                                                                                                                            cATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCtt                    >  2:1194440/1‑132 (MQ=255)                                                                                                                            cATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCtt                    <  1:1194440/132‑1 (MQ=255)                                                                                                                            cATGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTaa            <  2:960139/140‑1 (MQ=255)                                                                                                                              tGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAAc                          >  2:1028414/1‑124 (MQ=255)                                                                                                                              tGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAAc                          <  1:1028414/124‑1 (MQ=255)                                                                                                                              tGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTaa            <  2:610426/138‑1 (MQ=255)                                                                                                                              tGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTaa            >  1:610426/1‑138 (MQ=255)                                                                                                                              tGAGTAACCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACg          >  2:51539/1‑140 (MQ=255)                                                                                                                                    aCCCGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACgccagcc    <  1:974021/140‑1 (MQ=255)                                                                                                                                      ccGGCGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCAt  >  2:152700/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        GGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAGCGGGCGGCTGTCATTTTATAAAATAAAGTAGTCTCATGCTTCTTCTGTGAAGCATGAGTAACCCGGTGTTATTGCAGGCCATTATTCATTTTTCGCTACCAGCAAAGAGAGATCCTGCTTCACCAGCGCGCGACTGGCACTCTCCGGCAAACCGTCGTCTGTAATAATCTGATCAAACTCGCTTAATGGTAACGCCAGCCAT  >  NC_000913/2860315‑2860587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑