Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,895,838	T→C	T240A (ACG→GCG)	ygcR ←	putative flavoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,895,838	0	T	C	100.0%	170.3 / NA	48	T240A (ACG→GCG)	ygcR	putative flavoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/26);  total (22/26)

ACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTTCCCCGCAGCCATTCGCTGGCGCATTCC  >  NC_000913/2895702‑2895973                                                                                                                                         |                                                                                                                                        aCCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTg                                                                                                                                      <  2:877297/140‑1 (MQ=255)  ccGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGc                                                                                                                                     <  2:843474/140‑1 (MQ=255)     cTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCa                                                                                                                                  >  2:195712/1‑140 (MQ=255)     cTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCa                                                                                                                                  >  2:809072/1‑140 (MQ=255)     cTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCa                                                                                                                                  >  2:355373/1‑140 (MQ=255)     cTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCa                                                                                                                                  >  2:1261654/1‑140 (MQ=255)                     ccagccagCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTc                                                                                                                  <  2:873294/140‑1 (MQ=255)                          cagcTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGcgt                                                                                                             <  1:690604/140‑1 (MQ=255)                                 cTATTGCGGCATTCTCCTGATTTATTTTCCCAATTGCGATTTTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTtgct                                                                                                      <  1:1181459/140‑1 (MQ=255)                                 cTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTtgct                                                                                                      <  1:899347/140‑1 (MQ=255)                                 cTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTtgct                                                                                                      <  1:158687/140‑1 (MQ=255)                                 cTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTtgct                                                                                                      <  2:509220/140‑1 (MQ=255)                                         gCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCtt                                                                                              >  2:1323441/1‑140 (MQ=255)                                           aTTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGc                                                                                            <  2:720658/140‑1 (MQ=255)                                           aTTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGc                                                                                            <  1:809072/140‑1 (MQ=255)                                           aTTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGc                                                                                            <  1:355373/140‑1 (MQ=255)                                             tctcCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTACCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCa                                                                                          <  1:123889/140‑1 (MQ=255)                                             tctcCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGCCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCa                                                                                          <  1:715294/140‑1 (MQ=255)                                               tcCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGt                                                                                        <  1:246433/140‑1 (MQ=255)                                               tcCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGt                                                                                        <  2:137133/140‑1 (MQ=255)                                                  tGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGc                                                                                     <  1:1235143/140‑1 (MQ=255)                                                   gATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCa                                                                                    <  1:1360769/140‑1 (MQ=255)                                                   gATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCa                                                                                    <  1:1272445/140‑1 (MQ=255)                                                         ttGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACt                                                                              >  2:1184485/1‑140 (MQ=255)                                                         ttGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACt                                                                              >  2:1153148/1‑140 (MQ=255)                                                         ttGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACt                                                                              >  1:248397/1‑140 (MQ=255)                                                           gTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGc                                                                            <  1:1261654/140‑1 (MQ=255)                                                              acaATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATc                                                                         >  1:949981/1‑140 (MQ=255)                                                                                gCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGgcagca                                                       >  2:342556/1‑140 (MQ=255)                                                                                  tGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAAc                                                     >  1:926593/1‑140 (MQ=255)                                                                                    cATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCCGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAg                                                   >  1:162112/1‑140 (MQ=255)                                                                                         tttGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTa                                              <  2:949981/140‑1 (MQ=255)                                                                                                     agcccTGCCATAGCTTTTGACCTTTTTCTGCTCCCGTTTGCCCATCAACCAGCGTCGCTCCGCGTCGTTGCTCTGCTTTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCt                                  <  2:1077085/137‑1 (MQ=255)                                                                                                       tCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGc                                >  1:1128369/1‑140 (MQ=255)                                                                                                       tCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGc                                >  1:1193072/1‑140 (MQ=255)                                                                                                       tCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGc                                >  1:162518/1‑140 (MQ=255)                                                                                                           gCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCTATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTTc                            >  1:1311251/1‑140 (MQ=255)                                                                                                           gCCATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTTc                            >  2:733287/1‑140 (MQ=255)                                                                                                             cATAGCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTTccc                          <  2:346624/140‑1 (MQ=255)                                                                                                                 gCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTTCCCCGCa                      >  2:365242/1‑140 (MQ=255)                                                                                                                 gCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTTCCCCGCa                      >  1:743030/1‑140 (MQ=255)                                                                                                                 gCTTTTGAGCTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTTCCCCGCa                      >  1:866090/1‑140 (MQ=255)                                                                                                                          cTTTTTCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTTCCCCGCAGCCATTCGc             <  2:248397/140‑1 (MQ=255)                                                                                                                            ttttCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTTCCCCGCAg                     >  2:948906/1‑130 (MQ=255)                                                                                                                            ttttCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTTCCCCGCAg                     <  1:948906/130‑1 (MQ=255)                                                                                                                            ttttCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTTCCCCGCAGCCATTCGCTg           >  1:226131/1‑140 (MQ=255)                                                                                                                             tttCTGCTACCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTTCCCCGCAGCCATTCGCTgg          <  1:195712/140‑1 (MQ=255)                                                                                                                                     aCCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTTCCCCGCAGCCATTCGCTGGCGCATTcc  <  1:342556/140‑1 (MQ=255)                                                                                                                                     aCCGCTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTTCCCCGCAGCCATTCGCTGGCGCATTcc  <  2:162518/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        ACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTCTCCTGATTTATTGTCACAATTGCGATATTCATGGCTGCATCCTTTGGCGTAAATAGTCCTGCCATAGCTTTTGAGCTTTTTCTGCTACCGTTTGCCCATCAATCAGCGTCGCTCCGCGTCGTTGCTCTGCTCTTGCCAGTTGCAGACACTGCATCGCTGGCATTTCGGCAGCAACAGTTTTACGGATAATTTCTGCTTTCCCCGCAGCCATTCGCTGGCGCATTCC  >  NC_000913/2895702‑2895973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑