Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,905,388	T→C	T11A (ACC→GCC)	queE ←	putative 7‑carboxy‑7‑deazaguanine synthase QueE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,905,388	0	T	C	100.0%	288.8 / NA	85	T11A (ACC→GCC)	queE	putative 7‑carboxy‑7‑deazaguanine synthase QueE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/53);  total (32/53)

TGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGTCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCG  >  NC_000913/2905253‑2905520                                                                                                                                        |                                                                                                                                     tGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTgg                                                                                                                                  >  2:949357/1‑140 (MQ=255)      aGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACAt                                                                                                                             >  1:707297/1‑140 (MQ=255)          agaCTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGTATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCg                                                                                                                         >  1:1064308/1‑140 (MQ=255)          agaCTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCg                                                                                                                         >  2:448624/1‑140 (MQ=255)             cTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTa                                                                                                                      >  2:750951/1‑140 (MQ=255)              ttCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTaa                                                                                                                     <  1:412282/140‑1 (MQ=255)              ttCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTaa                                                                                                                     <  1:968581/140‑1 (MQ=255)               tCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAAt                                                                                                                    >  2:472395/1‑140 (MQ=255)                cccGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATc                                                                                                                   <  2:808059/140‑1 (MQ=255)                cccGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATc                                                                                                                   <  2:936992/140‑1 (MQ=255)                  cGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCgg                                                                                                                 <  2:936101/140‑1 (MQ=255)                   gATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCggg                                                                                                                <  2:384248/140‑1 (MQ=255)                   gATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCggg                                                                                                                <  1:569341/140‑1 (MQ=255)                   gATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCggg                                                                                                                <  2:1177530/140‑1 (MQ=255)                     tCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTa                                                                                                              <  1:1113751/140‑1 (MQ=255)                       cTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACt                                                                                                            <  1:949357/140‑1 (MQ=255)                       cTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACt                                                                                                            <  2:85473/140‑1 (MQ=255)                       cTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACt                                                                                                            <  2:842352/140‑1 (MQ=255)                       cTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACt                                                                                                            <  2:763630/140‑1 (MQ=255)                          aaGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTTGAACATCTCGTTAATCGGGTACTGCa                                                                                                         >  2:1015984/1‑140 (MQ=255)                          aaGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCa                                                                                                         >  1:1135474/1‑140 (MQ=255)                            gCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATa                                                                                                       <  2:1139610/140‑1 (MQ=255)                              ttttCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGc                                                                                                     <  1:135476/140‑1 (MQ=255)                               tttCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCa                                                                                                    >  1:856185/1‑140 (MQ=255)                               tttCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCa                                                                                                    <  1:45945/140‑1 (MQ=255)                               tttCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCa                                                                                                    >  1:1255460/1‑140 (MQ=255)                               tttCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCa                                                                                                    <  1:1190002/140‑1 (MQ=255)                                 tCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCAtt                                                                                                  <  1:448624/140‑1 (MQ=255)                                     aGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTctct                                                                                              <  2:343827/140‑1 (MQ=255)                                        tgtgtTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCtgtg                                                                                           <  1:472395/140‑1 (MQ=255)                                        tgtgtTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCtgtg                                                                                           <  1:1301671/140‑1 (MQ=255)                                        tgtgtTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCtgtg                                                                                           <  2:856185/140‑1 (MQ=255)                                          tgtTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGaa                                                                                         >  1:425848/1‑140 (MQ=255)                                          tgtTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGaa                                                                                         <  1:972714/140‑1 (MQ=255)                                          tgtTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGaa                                                                                         <  2:202525/140‑1 (MQ=255)                                          tgtTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGaa                                                                                         >  2:1018938/1‑140 (MQ=255)                                           gtTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAg                                                                                        <  1:1018938/140‑1 (MQ=255)                                            tttGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGt                                                                                       >  2:834905/1‑140 (MQ=255)                                            tttGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGt                                                                                       >  2:966844/1‑140 (MQ=255)                                             ttGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTg                                                                                      >  1:1315945/1‑140 (MQ=255)                                               ggTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGa                                                                                    >  1:1148187/1‑140 (MQ=255)                                                gtgtCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGAt                                                                                   <  2:461076/140‑1 (MQ=255)                                                 tgtCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATa                                                                                  >  1:820753/1‑140 (MQ=255)                                                 tgtCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATa                                                                                  >  1:1022141/1‑140 (MQ=255)                                                  gtCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCCCCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATaa                                                                                 <  1:843726/140‑1 (MQ=255)                                                    cGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAAtt                                                                               >  2:1068506/1‑140 (MQ=255)                                                     gCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCtgtg                                                                                           <  2:997844/127‑1 (MQ=255)                                                     gCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCtgtg                                                                                           >  1:997844/1‑127 (MQ=255)                                                      cACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGt                                                                             >  1:519130/1‑140 (MQ=255)                                                      cACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGt                                                                             >  1:1304284/1‑140 (MQ=255)                                                       aCCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGtt                                                                            <  2:978889/140‑1 (MQ=255)                                                              acaGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAattatt                                                                     <  2:202345/140‑1 (MQ=255)                                                                aGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAatt                                                                        <  1:1264497/135‑1 (MQ=255)                                                                aGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAatt                                                                        >  2:1264497/1‑135 (MQ=255)                                                                aGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGc                                                                   >  1:1275935/1‑140 (MQ=255)                                                                    aaCCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGAt                                                               <  1:966844/140‑1 (MQ=255)                                                                      ccGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATcc                                                             >  1:88093/1‑140 (MQ=255)                                                                         ggCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGc                                                          <  2:1351293/140‑1 (MQ=255)                                                                         ggCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGc                                                          <  2:1148187/140‑1 (MQ=255)                                                                              cccTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCacaa                                                     <  2:425848/140‑1 (MQ=255)                                                                              cccTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCacaa                                                     <  2:820753/140‑1 (MQ=255)                                                                                cTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGc                                                          <  1:836575/133‑1 (MQ=255)                                                                                cTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGc                                                          >  2:836575/1‑133 (MQ=255)                                                                                    aaaCGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCa                                               >  1:162252/1‑140 (MQ=255)                                                                                     aaCGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCAt                                              <  2:30089/140‑1 (MQ=255)                                                                                     aaCGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCAt                                              <  2:1064308/140‑1 (MQ=255)                                                                                     aaCGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCAt                                              <  2:713511/140‑1 (MQ=255)                                                                                      aCGAATAAAAATGGCGGGAACGCCGGTAAAGTACCCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATg                                             <  1:1015984/140‑1 (MQ=255)                                                                                      aCGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATg                                             <  1:26878/140‑1 (MQ=255)                                                                                       cGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGt                                            <  1:834905/140‑1 (MQ=255)                                                                                        gAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTc                                           >  1:251184/1‑140 (MQ=255)                                                                                         aaTAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGAGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCt                                          <  2:707297/140‑1 (MQ=255)                                                                                         aaTAAAAATGGCGGAAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCGTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCt                                          <  1:990895/140‑1 (MQ=255)                                                                                            aaaaaTGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCttat                                       >  1:675836/1‑140 (MQ=255)                                                                                                   gCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACAtt                                <  1:1040128/140‑1 (MQ=255)                                                                                                   gCGGGAACCCCGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCATTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACAtt                                <  2:1252059/140‑1 (MQ=255)                                                                                                             cGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTAcagg                      <  1:1068506/140‑1 (MQ=255)                                                                                                             cGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTAcagg                      <  1:750951/140‑1 (MQ=255)                                                                                                             cGGTAAAGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTAcagg                      <  2:1135474/140‑1 (MQ=255)                                                                                                                  aaGTAACCCTCACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGt                 <  2:475632/140‑1 (MQ=255)                                                                                                                            cACCTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGaaaa       >  1:1240832/1‑140 (MQ=255)                                                                                                                              ccTTGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAAt     >  1:355013/1‑140 (MQ=255)                                                                                                                                 tGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCg  >  2:599207/1‑140 (MQ=255)                                                                                                                                 tGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCg  <  2:355013/140‑1 (MQ=255)                                                                                                                                 tGCAGGGCCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCg  <  2:1022141/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     TGAAAAGGGAGACTTCCCGATCCTCAAGCTTTTCCCAGGTGTGTTTGGTGTCGCACCAGGCACAGCCAACCGGGCATCCCTGTAAACGAATAAAAATGGCGGGAACGCCGGTAAAGTAACCCTCACCTTGCAGGGTCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCG  >  NC_000913/2905253‑2905520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑