Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,212,088	(C)8→7	intergenic (‑85/+172)	iraM ← / ← ymgK	anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,212,081	0	C	.	100.0%	207.7 / NA	48	intergenic (‑78/+179)	iraM/ymgK	anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (24/24);  total (24/24)

ATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGT  >  NC_000913/1211942‑1212216                                                                                                                                            |                                                                                                                                        atGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTATTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAc                                                                                                                                         >  1:660363/1‑140 (MQ=255) atGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAc                                                                                                                                         >  2:514921/1‑140 (MQ=255)   ggCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAccc                                                                                                                                       >  2:528982/1‑140 (MQ=255)   ggCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAccc                                                                                                                                       >  1:81265/1‑140 (MQ=255)   ggCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAccc                                                                                                                                       <  1:514921/140‑1 (MQ=255)     cTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAccccc                                                                                                                                     >  1:1126257/1‑140 (MQ=255)          aaaGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGAAGTTTATTTCTTGATTTTGCATATGAGTATATTAccccccctca                                                                                                                                >  1:1104381/1‑137 (MQ=255)                    cacaTGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAaaataaa                                                                                                                     <  1:986659/140‑1 (MQ=255)                    cacaTGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAaaataaa                                                                                                                     <  1:787784/140‑1 (MQ=255)                       aTGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAaatta                                                                                                                  >  1:860571/1‑140 (MQ=255)                          tAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAaattaatt                                                                                                               <  2:1125404/140‑1 (MQ=255)                           aGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAattaatta                                                                                                              <  2:860571/140‑1 (MQ=255)                            ggTTGAATTACCGTGTCAATTACTATCCACTTCCTTTGTTATGTCTTATCCCACGGCATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAACATAAAttaattaa                                                                                                             >  2:186468/1‑140 (MQ=255)                             gTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTaaa                                                                                                            >  1:81605/1‑140 (MQ=255)                                gAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                                         <  1:528982/140‑1 (MQ=255)                                gAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                                         <  2:81605/140‑1 (MQ=255)                                 aaTTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAaatt                                                                                                                   <  2:189734/129‑1 (MQ=255)                                 aaTTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAaatt                                                                                                                   >  1:189734/1‑129 (MQ=255)                                 aaTTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAatga                                                                                                        >  2:322111/1‑140 (MQ=255)                                 aaTTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAatga                                                                                                        >  1:663277/1‑140 (MQ=255)                                 aaTTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAatga                                                                                                        >  1:828656/1‑140 (MQ=255)                                       cGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCtt                                                                                                  >  1:1119803/1‑140 (MQ=255)                                                     tCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAAGGATGGCTTATATaaaataaaat                                                                                    >  1:905773/1‑140 (MQ=255)                                                       cACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAAttt                                                                                  >  2:482476/1‑140 (MQ=255)                                                         cTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTaa                                                                                <  1:1173635/140‑1 (MQ=255)                                                         cTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTaa                                                                                >  2:905734/1‑140 (MQ=255)                                                            cATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGTGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGc                                                                             >  2:890119/1‑140 (MQ=255)                                                             aTTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCa                                                                            >  1:232983/1‑140 (MQ=255)                                                              tttGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCaa                                                                           <  1:938279/140‑1 (MQ=255)                                                                tGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAgg                                                                         <  2:663277/140‑1 (MQ=255)                                                                 gTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGa                                                                        <  2:1119803/140‑1 (MQ=255)                                                                   tATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAAt                                                                      <  2:660363/140‑1 (MQ=255)                                                                   tATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAAt                                                                      <  1:482476/140‑1 (MQ=255)                                                                         ttATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAAt                                                                <  2:828656/140‑1 (MQ=255)                                                                         ttATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAAt                                                                <  2:43589/140‑1 (MQ=255)                                                                            tCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGa                                                             >  1:112299/1‑140 (MQ=255)                                                                            tCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGa                                                             >  2:493714/1‑140 (MQ=255)                                                                                 cGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAAt                                                                >  1:315069/1‑132 (MQ=255)                                                                                 cGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAAt                                                                <  2:315069/132‑1 (MQ=255)                                                                                 cGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTa                                                        >  2:480414/1‑140 (MQ=255)                                                                                   gTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTaaa                                                      >  1:1052963/1‑140 (MQ=255)                                                                                   gTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTaaa                                                      <  1:1155622/140‑1 (MQ=255)                                                                                             tGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATgaga                                            >  2:1107731/1‑140 (MQ=255)                                                                                              ggTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGatat                                           >  1:717944/1‑140 (MQ=255)                                                                                              ggTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAt                                           >  1:1127984/1‑140 (MQ=255)                                                                                                    ttAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTtgtg                                     <  1:480414/140‑1 (MQ=255)                                                                                                    ttAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTtgtg                                     >  1:605646/1‑140 (MQ=255)                                                                                                       ggATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGaaa                                  >  2:784423/1‑140 (MQ=255)                                                                                                         aTGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGc                                >  1:65497/1‑140 (MQ=255)                                                                                                          tGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCGATGGATGTTAAACAAAATGAGATTTTGTGAAAGCa                               <  2:81265/140‑1 (MQ=255)                                                                                                            tttATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAAt                             >  1:634055/1‑140 (MQ=255)                                                                                                                       aTTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGAc                  <  1:784423/140‑1 (MQ=255)                                                                                                                        ttttGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACt                 <  2:605646/140‑1 (MQ=255)                                                                                                                        ttttGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACt                 <  2:1052963/140‑1 (MQ=255)                                                                                                                                      atatTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTg   <  1:546265/140‑1 (MQ=255)                                                                                                                                       tatTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGt  <  1:30220/140‑1 (MQ=255)                                                                                                                                       tatTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGt  >  2:1094518/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                        ATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGT  >  NC_000913/1211942‑1212216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑