Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	766,957	(G)6→7	coding (974/1977 nt)	mngA →	2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	766,951	1	.	G	100.0%	177.5 / NA	48	A323G (GCG→GGG)	mngA	2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (24/24);  total (24/24)

CAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑GGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGATGGTA  >  NC_000913/766818‑767084                                                                                                                                       |                                                                                                                                      caaGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑gggggg                                                                                                                                 >  1:1025337/1‑140 (MQ=255)  aaGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑ggggggg                                                                                                                                <  2:1173367/140‑2 (MQ=255)    gcgcTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑gggggggca                                                                                                                              <  2:897608/140‑4 (MQ=255)    gcgcTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑gggggggca                                                                                                                              <  1:1151518/140‑4 (MQ=255)       cTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACgg                                                                                                                            >  1:1138745/1‑140 (MQ=255)           cccTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCt                                                                                                                        <  2:1025337/140‑1 (MQ=255)                aaGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCgg                                                                                                                   >  2:111122/1‑140 (MQ=255)                   cGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTcg                                                                                                                <  2:1171650/140‑1 (MQ=255)                   cGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTcg                                                                                                                <  2:879149/140‑1 (MQ=255)                   cGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTcg                                                                                                                <  2:70903/140‑1 (MQ=255)                   cGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTcg                                                                                                                <  2:468708/140‑1 (MQ=255)                   cGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTcg                                                                                                                <  1:270889/140‑1 (MQ=255)                      aGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCgg                                                                                                             >  2:945467/1‑140 (MQ=255)                            gagaCGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTcg                                                                                                                <  1:444713/131‑1 (MQ=255)                            gagaCGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTcg                                                                                                                >  2:444713/1‑131 (MQ=255)                             agaCGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACt                                                                                                      >  1:133739/1‑140 (MQ=255)                                       cgtacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGGATTACTGTCGCAAATc                                                                                            >  2:24044/1‑140 (MQ=255)                                       cgtacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTAAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCGCGGTGCTGGCGGTCGCGGTATTATTGTCGCAAAtt                                                                                            >  2:881947/1‑139 (MQ=255)                                         tacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCTTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCtt                                                                                          <  1:930486/140‑1 (MQ=255)                                         tacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGTTGGCGGTCGCGGTATTACTGTCGCAAATCtt                                                                                          >  2:191643/1‑140 (MQ=255)                                         tacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCtt                                                                                          >  1:230470/1‑140 (MQ=255)                                         tacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCtt                                                                                          <  2:887824/140‑1 (MQ=255)                                         tacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCtt                                                                                          >  1:353817/1‑140 (MQ=255)                                         tacAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTCTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCtt                                                                                          <  2:617542/140‑1 (MQ=255)                                              cAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGGATTACTGTCGCAAATCTTCGGGc                                                                                     >  2:450486/1‑140 (MQ=255)                                                   tatgCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCTGGCTACaa                                                                                >  2:85495/4‑140 (MQ=255)                                                      gCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGAt                                                                             >  2:595538/1‑140 (MQ=255)                                                      gCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGAt                                                                             <  2:984570/140‑1 (MQ=255)                                                      gCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGAt                                                                             <  1:881947/140‑1 (MQ=255)                                                           cGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGtt                                                                        >  2:647835/1‑140 (MQ=255)                                                             gTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGGATTACTGTCGCAAATCTTCGGGCTACAAGATCTGtttt                                                                      >  2:230925/1‑139 (MQ=255)                                                                    gtgtCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATgaaga                                                               <  1:77384/140‑1 (MQ=255)                                                                           aaCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTcc                                                        <  2:230470/140‑1 (MQ=255)                                                                           aaCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTcc                                                        <  2:353817/140‑1 (MQ=255)                                                                            aCGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCt                                                       <  2:1138745/140‑1 (MQ=255)                                                                               gAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGc                                                    <  1:450486/140‑1 (MQ=255)                                                                               gAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGc                                                    <  2:111935/140‑1 (MQ=255)                                                                                 gCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGTTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCtg                                                  >  1:202167/1‑140 (MQ=255)                                                                                      aaCAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGTTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGAt                                             <  1:191643/140‑1 (MQ=255)                                                                                            cACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGGATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCg                                       >  1:733943/1‑140 (MQ=255)                                                                                              cTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCa                                     <  2:733943/140‑1 (MQ=255)                                                                                               tGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCaa                                    >  2:815037/1‑140 (MQ=255)                                                                                                 ttGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGGATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGc                                  >  2:1119239/1‑140 (MQ=255)                                                                                                         aaTCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGGATTAATGTCGCAAATCTTCGGGCTACAAGATCTGTTTTATGAAGAAAAATCCTGGCTGTGGGAGTATCGCCAAGTGggcggc                          >  2:941575/1‑140 (MQ=255)                                                                                                           tctcTTTTGCCGTACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGcggcgg                        >  1:1002237/1‑140 (MQ=255)                                                                                                                       tACCGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAAt            >  1:766366/1‑140 (MQ=255)                                                                                                                          cGTTGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAAtttt         <  1:945467/140‑1 (MQ=255)                                                                                                                            ttGATTGTCGCGGGGGGGCACGGTGTTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGa       <  2:202167/140‑1 (MQ=255)                                                                                                                            ttGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGa       <  1:202852/140‑1 (MQ=255)                                                                                                                            ttGATTGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGa       <  1:299296/140‑1 (MQ=255)                                                                                                                                 tGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGATGGTa  >  1:1085974/1‑140 (MQ=255)                                                                                                                                 tGTCGCGGGGGGGCACGGTGCTGGCGGTCGCGGGATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGGTGTACCGCAAGCTGGGGGGCGGGGTGCTCGGAATTTTGATGGTa  >  2:299296/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      CAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGC‑GGGGGGCACGGTGCTGGCGGTCGCGGTATTACTGTCGCAAATCTTCGGGCTACAAGATCTGTTTAATGAAGAAAACTCCTGGCTGTGGATGTACCGCAAGCTGGGCGGCGGGCTGCTCGGAATTTTGATGGTA  >  NC_000913/766818‑767084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑