Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,727,494	(G)6→7	coding (836/1098 nt)	nemA →	N‑ethylmaleimide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,727,488	1	.	G	100.0%	254.2 / NA	68	A277G (GCG→GGG)	nemA	N‑ethylmaleimide reductase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (41/27);  total (41/27)

TTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTG  >  NC_000913/1727350‑1727621                                                                                                                                            |                                                                                                                                      ttGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑g                                                                                                                                      <  2:280013/140‑1 (MQ=255) ttGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑g                                                                                                                                      <  1:208346/140‑1 (MQ=255)      aTTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑gggggg                                                                                                                                 <  1:563435/140‑1 (MQ=255)       ttCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑ggggggg                                                                                                                                >  2:917238/1‑139 (MQ=255)        tCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑gggggggt                                                                                                                               <  2:31740/140‑3 (MQ=255)        tCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑gggggggt                                                                                                                               <  1:19813/140‑3 (MQ=255)         cgcgTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑gggggggtg                                                                                                                              >  2:175870/1‑137 (MQ=255)         cgcgTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑gggggggtg                                                                                                                              <  1:917238/140‑4 (MQ=255)           cgTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑gggggggtgaa                                                                                                                            <  1:597554/140‑6 (MQ=255)           cgTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑gggggggtgaa                                                                                                                            <  2:509655/140‑6 (MQ=255)           cgTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑gggggggtgaa                                                                                                                            <  1:175870/140‑6 (MQ=255)           cgTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑gggggggtgaa                                                                                                                            <  1:155233/140‑6 (MQ=255)                     aTCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTg                                                                                                                   >  1:81391/1‑140 (MQ=255)                        ggTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATg                                                                                                                >  2:827278/1‑140 (MQ=255)                          tACTTTCCAGAACATAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCg                                                                                                              >  1:336058/1‑140 (MQ=255)                          tACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCg                                                                                                              <  2:336058/140‑1 (MQ=255)                                 cAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCcgcg                                                                                                       >  1:487850/1‑140 (MQ=255)                                    aaCACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGaaa                                                                                                    >  2:828634/1‑140 (MQ=255)                                    aaCACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGaaa                                                                                                    <  1:957031/140‑1 (MQ=255)                                    aaCACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGaaa                                                                                                    <  1:589076/140‑1 (MQ=255)                                         aGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTTAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTa                                                                                               >  2:866617/1‑140 (MQ=255)                                         aGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTa                                                                                               >  1:727636/1‑140 (MQ=255)                                         aGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTa                                                                                               >  1:876393/1‑140 (MQ=255)                                         aGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTa                                                                                               >  1:324635/1‑140 (MQ=255)                                             aaCGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTAcgcg                                                                                           <  1:793774/140‑1 (MQ=255)                                             aaCGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTAcgcg                                                                                           <  2:81391/140‑1 (MQ=255)                                                  cccGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGt                                                                                      >  2:779319/1‑140 (MQ=255)                                                   ccGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGtt                                                                                     >  1:104985/1‑140 (MQ=255)                                                     gAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTc                                                                                   >  2:874561/1‑140 (MQ=255)                                                          aagaagCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACgg                                                                              >  1:50139/1‑140 (MQ=255)                                                                   aTGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTAt                                                                     >  1:88676/1‑140 (MQ=255)                                                                    tGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACg                                                                               <  1:1052186/129‑1 (MQ=255)                                                                    tGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACg                                                                               >  2:1052186/1‑129 (MQ=255)                                                                        cTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGGATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCGGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGgcg                                                                >  2:880740/1‑140 (MQ=255)                                                                        cTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGgcg                                                                <  2:727636/140‑1 (MQ=255)                                                                          gTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCa                                                              <  2:162449/140‑1 (MQ=255)                                                                          gTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCa                                                              >  2:572738/1‑140 (MQ=255)                                                                          gTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCa                                                              <  2:613831/140‑1 (MQ=255)                                                                          gTATCAGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCa                                                              <  1:982090/140‑1 (MQ=255)                                                                                  ttGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTTAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATa                                                      <  1:866617/140‑1 (MQ=255)                                                                                   tGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATac                                                     >  2:681281/1‑140 (MQ=255)                                                                                   tGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATac                                                     >  1:588492/1‑140 (MQ=255)                                                                                     aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCa                                                              <  1:114318/129‑1 (MQ=255)                                                                                     aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCa                                                              >  2:114318/1‑129 (MQ=255)                                                                                     aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATacac                                                   >  2:1058383/1‑140 (MQ=255)                                                                                     aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATacac                                                   >  2:219752/1‑140 (MQ=255)                                                                                     aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATacac                                                   <  2:588492/140‑1 (MQ=255)                                                                                     aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATacac                                                   >  1:1006992/1‑140 (MQ=255)                                                                                     aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATacac                                                   >  1:1229945/1‑140 (MQ=255)                                                                                     aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATacac                                                   >  1:155709/1‑140 (MQ=255)                                                                                     aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATacac                                                   >  1:192739/1‑140 (MQ=255)                                                                                     aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATa                                                      <  2:684396/137‑1 (MQ=255)                                                                                     aacaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATa                                                      >  1:684396/1‑137 (MQ=255)                                                                                      acaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATAcaca                                                  <  2:845108/140‑1 (MQ=255)                                                                                       caacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATa                                                      <  2:801379/135‑1 (MQ=255)                                                                                       caacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATa                                                      >  1:801379/1‑135 (MQ=255)                                                                                                  aCGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCt                                      >  2:105770/1‑140 (MQ=255)                                                                                                       gCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAAc                                 >  2:928042/1‑140 (MQ=255)                                                                                                            gCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGa                            <  1:827278/140‑1 (MQ=255)                                                                                                            gCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGa                            <  1:536442/140‑1 (MQ=255)                                                                                                             cTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGAt                           <  1:779319/140‑1 (MQ=255)                                                                                                             cTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGAt                           <  1:681281/140‑1 (MQ=255)                                                                                                                 tCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGc                       <  2:324635/140‑1 (MQ=255)                                                                                                                    gCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCaaa                    >  1:632182/1‑140 (MQ=255)                                                                                                                     cATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGAt                           >  2:456069/1‑132 (MQ=255)                                                                                                                     cATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGAt                           <  1:456069/132‑1 (MQ=255)                                                                                                                     cATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAg                   >  1:227954/1‑140 (MQ=255)                                                                                                                      atatGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAgg                  <  2:632182/140‑1 (MQ=255)                                                                                                                       tatGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAggg                 >  1:83578/1‑140 (MQ=255)                                                                                                                        atGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGt                >  1:912361/1‑140 (MQ=255)                                                                                                                          gTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTa              >  2:580095/1‑140 (MQ=255)                                                                                                                           tCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTaa             >  1:525044/1‑140 (MQ=255)                                                                                                                             aGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAAtt           >  1:606413/1‑140 (MQ=255)                                                                                                                                aCCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGAATATCGGCGCAGGTGCATGAACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGAt        <  2:876393/140‑1 (MQ=255)                                                                                                                                 ccAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATg       <  2:1006992/140‑1 (MQ=255)                                                                                                                                  cAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGc      >  2:1027209/1‑140 (MQ=255)                                                                                                                                    gATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCgg    <  1:232956/140‑1 (MQ=255)                                                                                                                                     aTTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCgg    <  1:1041167/139‑1 (MQ=255)                                                                                                                                     aTTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCgg    >  2:1041167/1‑139 (MQ=255)                                                                                                                                     aTTGGGCGGGGGGGTGAACCGTATACT‑ATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTg  >  2:999413/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                      TTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTG  >  NC_000913/1727350‑1727621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑