Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913646,7600TC27.8% 62.1 / 11.9 36K250K (AAA→AAG) citGtriphosphoribosyl‑dephospho‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base T (0/26);  new base C (10/0);  total (10/26)
Fisher's exact test for biased strand distribution p-value = 3.93e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCC  >  NC_000913/646623‑646898
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ttAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCttt                                                                                                                                          <  1:1074577/140‑1 (MQ=255)
    ataatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGc                                                                                                                                      <  1:808119/140‑1 (MQ=255)
       atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCaat                                                                                                                                   <  2:1269376/140‑1 (MQ=255)
       atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATGCCCCCctttttttaa                                                                                                                                   >  1:317222/1‑135 (MQ=255)
        ttAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCaata                                                                                                                                  <  2:1069355/140‑1 (MQ=255)
         tAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGCCCCCctttttttataa                                                                                                                                 >  2:350203/1‑133 (MQ=255)
                gtgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATtttttt                                                                                                                          >  1:371899/1‑138 (MQ=255)
                gtgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGACTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGTATGCCCCCCTTTTGTGATAAAGTTTgg                                                                                                                          >  1:445250/1‑139 (MQ=255)
                 tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTgcg                                                                                                                         <  2:153085/140‑1 (MQ=255)
                 tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATTTTTgcc                                                                                                                         >  1:531769/1‑139 (MQ=255)
                     aaaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTc                                                                                                                     <  2:1002772/140‑1 (MQ=255)
                      aaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAAGGCCCCCTTTTTTAATAAAATTTTCGCCTcc                                                                                                                    >  2:430622/1‑139 (MQ=255)
                          ccAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCt                                                                                                                <  2:769686/140‑1 (MQ=255)
                              gTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAATGTTTTTGCCTCCCGCTGTAg                                                                                                            >  1:113913/1‑140 (MQ=255)
                                   gATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAgc                                                                                                       <  1:939118/140‑1 (MQ=255)
                                       aGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGGCCCCCTTTTTCAATAAATTTTTTGCCCCCCCCTGTAGCCAgcgccg                                                                                                   >  1:170500/1‑138 (MQ=255)
                                       aGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTTAATAATTTTTTCGCCCCCCGCTGTAGCCCgcgccg                                                                                                   >  2:448300/1‑138 (MQ=255)
                                          aGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGcc                                                                                                <  1:1084862/140‑1 (MQ=255)
                                          aGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGcc                                                                                                <  1:688354/140‑1 (MQ=255)
                                             tCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGGCCCCCTTTTTCAATAATGTTTTCGTCTCGCGCCGTAGCCCGCGCCGGccccc                                                                                             >  2:688354/1‑140 (MQ=255)
                                             tCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGGCCCCCTTTTTCAATAATATTTTTGCCTCGCGCCGTTGCCAGCGGCGGccccc                                                                                             >  2:62891/1‑140 (MQ=255)
                                                         ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcg                                                                                 <  1:625235/140‑1 (MQ=255)
                                                         ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcg                                                                                 <  1:398261/140‑1 (MQ=255)
                                                                                   cTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGa                                                       <  2:317222/140‑1 (MQ=255)
                                                                                    tCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGCCCCCCTTTTTCAATAAATTTTTTGCCTCCCGCTGTTGCCCGCGCCGGCCCCCCCCCCCCCCCCCCGCTGCCACCGTGGGATCGccccttat                                                      >  2:236197/1‑134 (MQ=255)
                                                                                       cTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATGCCCCCCTTTTTTTATAAAGTTTTCGCCTTCCGCCGTTGCCAACGCAAGCCCCCCCCCCCCCCCCGCGCTGCGACGCAGGGATAGCCGGTggttgg                                                   >  2:1219180/1‑135 (MQ=255)
                                                                                        tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGcc                                                  <  1:350203/140‑1 (MQ=255)
                                                                                         gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCa                                                 <  1:12339/140‑1 (MQ=255)
                                                                                         gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCCTTTTTTAATAATTTTTTCGCCCCCCGCTGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCCCGATGCAACGCTGGTATCGCCGTTGATCGccc                                                 >  2:747059/1‑139 (MQ=255)
                                                                                         gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCCGATGCCCCCCTTTTTCCATAATATTTTTGCCTCCCGCCGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCCCCGCGCAACCCTGGGATCGCCGCTGGTCGccc                                                 >  2:12339/1‑139 (MQ=255)
                                                                                          tCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCAt                                                <  1:1219180/140‑1 (MQ=255)
                                                                                             aaCTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATTGCCATCAg                                             <  1:1236387/140‑1 (MQ=255)
                                                                                              aCTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt                                            <  1:342049/140‑1 (MQ=255)
                                                                                              aCTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt                                            <  2:445250/140‑1 (MQ=255)
                                                                                              aCTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGCCCCCCTTTTTCAATAAATTTTTCGCCCCCCGCCGTAGGCAGCGCCGGCCCCCCCCCCCCCCCCCCCGTGCAACGTTGGGATCGCCGCTGATCGCCCTCAGt                                            >  2:340452/1‑140 (MQ=255)
                                                                                              aCTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTCAATAAAGTTTTCGCCTCCCGCTGTTGCCAGCGCAGGCCCCCCCCCCCCCCCCCCGCTGCAACGCTGGTATCGCCGTTGATCGCCCTCAgg                                            >  2:841496/1‑139 (MQ=255)
                                                                                                tGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAg                                          <  2:1049897/140‑1 (MQ=255)
                                                                                                tGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAg                                          <  2:730328/140‑1 (MQ=255)
                                                                                                tGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAg                                          <  1:448300/140‑1 (MQ=255)
                                                                                                          aaTCGAGATCGGCGGGGGGTCGAATGCCCCCCTTTTTTAATAAATTTTTTGCCCCCCGCTGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCGCTGCAACGGTGGGATCGCCGTTGGTCGCCCTCAGTAGGAGCAAggga                                >  2:1052223/1‑138 (MQ=255)
                                                                                                           aTCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTAt                               <  1:747059/140‑1 (MQ=255)
                                                                                                                     gcggGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGGTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGc                     <  1:23498/140‑1 (MQ=255)
                                                                                                                      cggGGGGTCGAATGCCCCCCTTTTTCAATAATTTTTTCGCCTCCCGCTGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCGCGATGCAACGCTGGGATCGCCGTTGATCCCCATCAGTAGGAGCAAGGGATCGAGCAATGcc                    >  2:332818/1‑140 (MQ=255)
                                                                                                                             tCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAg             <  1:430622/140‑1 (MQ=255)
                                                                                                                                        cTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAAcccc  <  1:236197/140‑1 (MQ=255)
                                                                                                                                        cTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAAcccc  <  1:332818/140‑1 (MQ=255)
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TTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCC  >  NC_000913/646623‑646898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑