Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,721,378	0	G	A	25.0%	142.1 / 45.4	76	E476K (GAA→AAA)	patZ	peptidyl‑lysine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (29/28);  new base A (8/11);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 6.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGAAGCGGTGCATATTGCCGAACAGATTGGTTATCCGGTGGCG  >  NC_000913/2721240‑2721515                                                                                                                                           |                                                                                                                                          gaaggaaCCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGa                                                                                                                                          >  1:1170463/1‑140 (MQ=255) gaaggaaCCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGa                                                                                                                                          <  2:112902/140‑1 (MQ=255) gaaggaaCCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGa                                                                                                                                          >  1:1194060/1‑140 (MQ=255) gaaggaaCCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGa                                                                                                                                          <  2:558887/140‑1 (MQ=255)       aCCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGc                                                                                                                                    >  1:20018/1‑140 (MQ=255)                gCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCt                                                                                                                           >  1:199641/1‑140 (MQ=255)                   tttATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCAAAGGGGCTACGTCGCTCGa                                                                                                                        <  1:874355/140‑1 (MQ=255)                      aTGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATAc                                                                                                                     >  1:513189/1‑140 (MQ=255)                      aTGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCAAAGGGGCTACGTCGCTCGATAc                                                                                                                     <  2:484000/140‑1 (MQ=255)                          atatGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCAt                                                                                                                 <  1:1072402/140‑1 (MQ=255)                          atatGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCAt                                                                                                                 <  1:482645/140‑1 (MQ=255)                           tatGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCt                                                                                                                           >  1:133590/1‑129 (MQ=255)                           tatGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCt                                                                                                                           <  2:133590/129‑1 (MQ=255)                              ggtggAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGa                                                                                                                        >  2:221827/1‑129 (MQ=255)                              ggtggAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGa                                                                                                                        <  1:221827/129‑1 (MQ=255)                              ggtggAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAg                                                                                                             >  1:488366/1‑140 (MQ=255)                              ggtggAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAg                                                                                                             >  2:743070/1‑140 (MQ=255)                                     tACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGcc                                                                                                      <  2:513189/140‑1 (MQ=255)                                     tACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGcc                                                                                                      >  1:350862/1‑140 (MQ=255)                                     tACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGcc                                                                                                      >  2:678097/1‑140 (MQ=255)                                     tACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCAAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGcc                                                                                                      >  2:765837/1‑140 (MQ=255)                                      aCCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGccc                                                                                                     >  2:932619/1‑140 (MQ=255)                                       ccGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCa                                                                                                    >  1:811373/1‑140 (MQ=255)                                       ccGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCAAAGGGGCTACGTCGCTCGATCCCCATGAAGTTCAGCCCa                                                                                                    <  2:476085/140‑1 (MQ=255)                                       ccGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCAAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCa                                                                                                    <  2:539449/140‑1 (MQ=255)                                       ccGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCACCTTCTGTGGCAACAGGCGATTGCCAAAGGGGCTACGCCGCTCGATACCCATTAAGTGCAGCCCa                                                                                                    >  1:900596/1‑140 (MQ=255)                                          gCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATcc                                                                                                 >  2:335255/1‑140 (MQ=255)                                          gCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCAAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATcc                                                                                                 >  2:535588/1‑140 (MQ=255)                                            gTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCAAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTg                                                                                               <  1:765837/140‑1 (MQ=255)                                            gTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCAAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTg                                                                                               <  1:89595/140‑1 (MQ=255)                                             tAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCAAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGc                                                                                              >  1:645297/1‑140 (MQ=255)                                                  aGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCg                                                                                         <  2:488366/140‑1 (MQ=255)                                                    aaGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTa                                                                                       <  1:743070/140‑1 (MQ=255)                                                    aaGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCAAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTa                                                                                       >  1:752028/1‑140 (MQ=255)                                                       cAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATgg                                                                                    <  2:811373/140‑1 (MQ=255)                                                         aCTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCa                                                                                  >  1:427162/1‑140 (MQ=255)                                                           tACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATg                                                                                <  1:678097/140‑1 (MQ=255)                                                                gAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAacac                                                                           <  1:932619/140‑1 (MQ=255)                                                                 aaaCGTCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCAAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACg                                                                          <  2:1134238/140‑1 (MQ=255)                                                                  aaCGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGc                                                                         <  2:20018/140‑1 (MQ=255)                                                                  aaCGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCAAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGc                                                                         >  2:751365/1‑140 (MQ=255)                                                                   aCGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCt                                                                        <  1:605815/140‑1 (MQ=255)                                                                    cGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTc                                                                       >  1:1111468/1‑140 (MQ=255)                                                                        ggCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCAAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTa                                                                   <  1:535588/140‑1 (MQ=255)                                                                                ccAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGAtt                                                           <  1:359993/140‑1 (MQ=255)                                                                                   gCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGcc                                                        <  1:1180368/140‑1 (MQ=255)                                                                                   gCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCAAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGcc                                                        <  1:649788/140‑1 (MQ=255)                                                                                        cTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGa                                                   <  2:1111468/140‑1 (MQ=255)                                                                                        cTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCAAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGa                                                   <  2:752028/140‑1 (MQ=255)                                                                                            cTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGc                                               >  2:127881/1‑140 (MQ=255)                                                                                            cTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCAAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGc                                               >  2:758667/1‑140 (MQ=255)                                                                                               ccAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGAt                                                  >  1:277680/1‑134 (MQ=255)                                                                                               ccAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGAt                                                  <  2:277680/134‑1 (MQ=255)                                                                                                cAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCAGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCg                                           <  2:1194060/140‑1 (MQ=255)                                                                                                 aaTACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCACCCTACCTGGATTGCCAGCGATAGCACCGa                                          >  2:1005300/1‑140 (MQ=255)                                                                                                     ccGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGAAGCg                                      <  2:1265887/140‑1 (MQ=255)                                                                                                       gCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTTGATTGCCAGCGATAGCACCGAAGCGGt                                    <  2:427162/140‑1 (MQ=255)                                                                                                       gCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGaa                                         >  2:408730/1‑135 (MQ=255)                                                                                                       gCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGaa                                         <  1:408730/135‑1 (MQ=255)                                                                                                       gCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGAAGCGGt                                    <  2:199641/140‑1 (MQ=255)                                                                                                        cAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGAAGCg                                      <  1:327215/137‑1 (MQ=255)                                                                                                        cAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGAAGCg                                      >  2:327215/1‑137 (MQ=255)                                                                                                        cAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGAAGCGGTg                                   >  1:1163668/1‑140 (MQ=255)                                                                                                        cAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCAAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGAAGCGGTg                                   >  1:729453/1‑140 (MQ=255)                                                                                                          gAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGAAGCGGTGCa                                 <  1:889322/140‑1 (MQ=255)                                                                                                          gAAGCGCATCTTCTGTTGCAACAGGCGATTGCCAAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGAAGCGGTGCa                                 <  2:729453/140‑1 (MQ=255)                                                                                                          gAAGCGCATCTTCTGTTGAAACAGGCGATTGCCAAAGGGGCTACGTCGCTCGATCCCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGAAGCGGTGCa                                 <  2:900596/140‑1 (MQ=255)                                                                                                           aaGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGaa                                         >  2:1161591/1‑131 (MQ=255)                                                                                                           aaGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGaa                                         <  1:1161591/131‑1 (MQ=255)                                                                                                                     tctGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGAAGCGGTGCATATTGCCGAAc                      >  2:456405/1‑140 (MQ=255)                                                                                                                         ttGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGAAGCGGTGCATATTGCCGAACAGAt                  >  2:268131/1‑140 (MQ=255)                                                                                                                              aCAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGAAGCGGTGCATATTGCCGAACAGAt                  <  1:422652/135‑1 (MQ=255)                                                                                                                              aCAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGAAGCGGTGCATATTGCCGAACAGAt                  >  2:422652/1‑135 (MQ=255)                                                                                                                              aCAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGAAGCGGTGCATATTGCCGAACAGATTGGtt             >  1:544958/1‑140 (MQ=255)                                                                                                                                 ggCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGAAGCGGTGCATATTGCCGAACAGATTGGTTATc          >  1:342816/1‑140 (MQ=255)                                                                                                                                   cGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGAAGCGGTGCATATTGCCGAACAGATTGGTTATCCg        >  1:378565/1‑140 (MQ=255)                                                                                                                                         ccGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGAAGCGGTGCATATTGCCGAACAGATTGGTTATCCGGTGgcg  <  2:378565/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                          GAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACCTGGATTGCCAGCGATAGCACCGAAGCGGTGCATATTGCCGAACAGATTGGTTATCCGGTGGCG  >  NC_000913/2721240‑2721515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑