Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT32.5% 50.9 / 21.3 40intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (2/25);  new base T (13/0);  total (15/25)
Fisher's exact test for biased strand distribution p-value = 8.73e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGA  >  NC_000913/3206297‑3206566
                                                                                                                                           |                                                                                                                                   
cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTcc                                                                                                                                    <  2:511094/140‑1 (MQ=255)
   aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGa                                                                                                                                 >  2:1023783/1‑140 (MQ=255)
             ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACaaa                                                                                                                       >  1:1201166/1‑140 (MQ=255)
               cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACaaaaa                                                                                                                     >  1:490584/1‑140 (MQ=255)
                ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAAc                                                                                                                    <  2:490584/140‑1 (MQ=255)
                ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGATTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAAc                                                                                                                    <  1:1135898/140‑1 (MQ=255)
                  tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCACACAAAAACAAAAAATg                                                                                                                  >  2:471458/1‑140 (MQ=255)
                  tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTg                                                                                                                  <  1:478333/140‑1 (MQ=255)
                    ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGa                                                                                                                <  2:173957/140‑1 (MQ=255)
                         gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCACACAAATACAAAAAATGGAggttg                                                                                                           >  1:692250/1‑136 (MQ=255)
                              atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatga                                                                                                      <  1:153669/140‑1 (MQ=255)
                              atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatga                                                                                                      <  1:471458/140‑1 (MQ=255)
                                   cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGTTGGCATGATGAgg                                                                                                 >  1:1179409/1‑139 (MQ=255)
                                     ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                               <  2:975208/140‑1 (MQ=255)
                                          aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAAAGGAGGTGGCAAGATGAGCGaaaaga                                                                                          >  2:352521/1‑137 (MQ=255)
                                          aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTTAAAAAAAAAAAAAAAATGGGGGTTCCATGATGAGCGgaaaaa                                                                                          >  2:290786/1‑137 (MQ=255)
                                            tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                        <  1:197094/140‑1 (MQ=255)
                                            tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                        <  1:376502/140‑1 (MQ=255)
                                                      aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                              <  1:54272/140‑1 (MQ=255)
                                                       ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                             <  2:1260558/140‑1 (MQ=255)
                                                           cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTg                                                                         <  1:130469/140‑1 (MQ=255)
                                                                tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑CAATTTTTCAGACAAAAACAAAAAATGGAGGTTCCCTGGTGAGCGAAAATAATAAACAAAAACCAATGAATaa                                                                    >  2:130469/1‑140 (MQ=255)
                                                                  ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGTAATTTTTCCTG‑CAAAAACAAAAAATTGAGGTGTGATGATGAGCGAAAATAATAAACAAAAAGCAGGGGATaaat                                                                  >  2:732972/1‑138 (MQ=255)
                                                                          atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAAACAAAAAAAAAAAATGGAGGTTCCCTGTTGAGGGGAAATAAAAAACAAAAAGCCGTGAAAAAAtttgaagag                                                          >  2:712829/1‑133 (MQ=255)
                                                                          atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGGTGGCATGATGAGCGAAAAAAAAAAACAAAAGGCAGTGAATAAAATGACagaa                                                          >  2:143172/1‑139 (MQ=255)
                                                                           tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                         <  2:376788/140‑1 (MQ=255)
                                                                            atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAACAAAAAAAAAAAAAGGAGGTGCCCTGATGAGGGAAAGGAAAAAAAAAAAAGCAGGGAAAAAATTGaaaaaaaa                                                        >  2:1089430/1‑133 (MQ=255)
                                                                                   gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAATGGAGTGGCCATGATGGGGGAAAGGAAAAAGCAAAAAGCAGAGAATAAGATGACAGAGATTGTCGCt                                                 >  2:1146766/1‑140 (MQ=255)
                                                                                   gTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATCCAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                 <  2:546744/140‑1 (MQ=255)
                                                                                          aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTa                                          <  1:143172/140‑1 (MQ=255)
                                                                                                taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAATGGAGTTGCCATGATGAGCGAAAGAAAAAAACAACAAGCAGGGAAAAAATTGACAAAGATTGTCGCTAACTTTACCGccc                                    >  2:159819/1‑139 (MQ=255)
                                                                                                    aCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAt                                <  1:352521/140‑1 (MQ=255)
                                                                                                    aCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAt                                <  1:159819/140‑1 (MQ=255)
                                                                                                    aCCACATTTTTAGTGGTAGTTTGGGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAt                                <  1:1245751/140‑1 (MQ=255)
                                                                                                      cacaTTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAttt                              <  1:599936/140‑1 (MQ=255)
                                                                                                        caTTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCt                            <  1:1023783/140‑1 (MQ=255)
                                                                                                         aTTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTa                           <  2:1179409/140‑1 (MQ=255)
                                                                                                          tttttAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTAc                          <  1:290786/140‑1 (MQ=255)
                                                                                                               aGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCATGATGAGCGAAAGAAAAAAGCAACAGGCAGTGAATAAATTGACAAAGATTGTCGCTAACTTTTCCCCCCTGATTTTTTCCCCaa                     >  2:450078/1‑140 (MQ=255)
                                                                                                               aGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                     <  1:538006/140‑1 (MQ=255)
                                                                                                                gTGGTAGTTGGGGGAGGTG‑ATTTTTCCCAGACAAAAACAAAAGTTGGTTTTCCCATAATGAGAGAAGGTAAAAAGAACCAGGCAGTCAGTAAGTTGACAAAGATTTTCTCTACCTTTCCCGCCATGATTTCTACCCGAAt                    <  2:692250/140‑1 (MQ=255)
                                                                                                                   gTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAAAAAAAAAAAAGGAGGTGGCATGATGAGGGGAAAGAATAAACAAAAAGCAGTGAATAAATTGACAGAGATTGTCGCTAACTTTTCCGCCATGATTTCTACCCCAATGcc                 >  2:1125265/1‑140 (MQ=255)
                                                                                                                     aGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAAATGGGGGTGGCATGATGAGGGGAAATAAAAAACAAAAAGCAGGGAAAAAATTGACAGAGAATGTCGCTTACTTTACCCCCCTGATTTTTACCCGAATGCCtg               >  2:697733/1‑140 (MQ=255)
                                                                                                                     aGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAGTTGGCCTGATTAGCGAAAAGAATAAACAAAAAGCAGTGAAAAAATTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTTTACCCCAATGCCtg               >  2:538006/1‑140 (MQ=255)
                                                                                                                                  g‑AATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa  <  2:366512/140‑1 (MQ=255)
                                                                                                                                  g‑AATTTTTCAAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa  <  2:1201166/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                   
CAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGA  >  NC_000913/3206297‑3206566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑