Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,169,857	G→A	M10I (ATG→ATA)	qseB →	DNA‑binding transcriptional activator QseB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,169,857	0	G	A	100.0%	303.1 / NA	85	M10I (ATG→ATA)	qseB	DNA‑binding transcriptional activator QseB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/43);  total (42/43)

AGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAAC  >  NC_000913/3169722‑3169985                                                                                                                                        |                                                                                                                                 aGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGa                                                                                                                              <  1:38130/140‑1 (MQ=255) aGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGa                                                                                                                              >  2:853915/1‑140 (MQ=255) aGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGa                                                                                                                              <  1:940727/140‑1 (MQ=255)   tCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGAtt                                                                                                                            >  1:1141701/1‑140 (MQ=255)   tCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGAtt                                                                                                                            >  1:652609/1‑140 (MQ=255)   tCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGAtt                                                                                                                            >  1:516717/1‑140 (MQ=255)     cTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTgg                                                                                                                          >  1:549567/1‑140 (MQ=255)        aacaacTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGa                                                                                                                              <  1:553569/133‑1 (MQ=255)        aacaacTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGa                                                                                                                              >  2:553569/1‑133 (MQ=255)        aacaacTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGa                                                                                                                       >  2:1087176/1‑140 (MQ=255)        aacaacTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGa                                                                                                                       >  1:120155/1‑140 (MQ=255)         acaacTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGAc                                                                                                                      <  2:1104702/140‑1 (MQ=255)             cttcttAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCa                                                                                                                  >  2:478944/1‑140 (MQ=255)             cttcttAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCa                                                                                                                  >  1:1251118/1‑140 (MQ=255)             cttcttAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCa                                                                                                                  >  2:798536/1‑140 (MQ=255)             cttcttAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCa                                                                                                                  >  1:569619/1‑140 (MQ=255)              ttcttAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCAt                                                                                                                 <  1:478944/140‑1 (MQ=255)              ttcttAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCAt                                                                                                                 <  2:93073/140‑1 (MQ=255)                        aaaaaaaTAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGG‑TTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGc                                                                                                       <  2:223046/139‑1 (MQ=255)                        aaaaaaaTAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGG‑TTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGc                                                                                                       >  1:223046/1‑139 (MQ=255)                         aaaaaaTAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTCATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGcc                                                                                                      <  2:1141701/140‑1 (MQ=255)                           aaaataaaatTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCtt                                                                                                    <  2:217987/140‑1 (MQ=255)                                 aaatTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTaaa                                                                                              <  2:59727/140‑1 (MQ=255)                                             gTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGc                                                                                  <  1:928690/140‑1 (MQ=255)                                             gTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGc                                                                                  <  1:853915/140‑1 (MQ=255)                                                  gagCGCGTTACAACACGGTTTTCTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             <  1:640195/140‑1 (MQ=255)                                                  gagCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             <  1:288968/140‑1 (MQ=255)                                                  gagCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             <  1:768761/140‑1 (MQ=255)                                                  gagCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATAACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             >  2:681730/1‑140 (MQ=255)                                                   agCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATg                                                                                           >  2:1029521/1‑125 (MQ=255)                                                   agCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATg                                                                                           <  1:1029521/125‑1 (MQ=255)                                                   agCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             >  1:570313/1‑139 (MQ=255)                                                   agCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             <  2:570313/139‑1 (MQ=255)                                                   agCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             <  1:1227767/139‑1 (MQ=255)                                                   agCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             >  2:1227767/1‑139 (MQ=255)                                                   agCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGAc                                                                            >  2:703059/1‑140 (MQ=255)                                                     cgcgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATGGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             <  1:640539/137‑1 (MQ=255)                                                     cgcgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATGGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             >  2:640539/1‑137 (MQ=255)                                                     cgcgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             >  2:288051/1‑137 (MQ=255)                                                     cgcgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             <  1:288051/137‑1 (MQ=255)                                                     cgcgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGAc                                                                            >  1:1273383/1‑138 (MQ=255)                                                     cgcgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGAc                                                                            <  2:1273383/138‑1 (MQ=255)                                                     cgcgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTg                                                                          >  1:489202/1‑140 (MQ=255)                                                     cgcgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTg                                                                          >  1:246625/1‑140 (MQ=255)                                                       cgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGt                                                                        <  2:652609/140‑1 (MQ=255)                                                       cgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGt                                                                        <  2:1024432/140‑1 (MQ=255)                                                        gTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             <  1:392109/134‑1 (MQ=255)                                                        gTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             >  2:392109/1‑134 (MQ=255)                                                        gTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGtt                                                                       >  2:100201/1‑140 (MQ=255)                                                        gTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGtt                                                                       >  1:364347/1‑140 (MQ=255)                                                        gTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGtt                                                                       >  2:855579/1‑140 (MQ=255)                                                        gTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGtt                                                                       >  1:926003/1‑140 (MQ=255)                                                                acGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGAAGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAg                                                               <  2:569619/140‑1 (MQ=255)                                                                    tttACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGgtcg                                                           >  2:584100/1‑140 (MQ=255)                                                                    tttACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGgtcg                                                           <  2:278503/140‑1 (MQ=255)                                                                    tttACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGgtcg                                                           >  2:640019/1‑140 (MQ=255)                                                                    tttACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGgtcg                                                           >  1:278503/1‑140 (MQ=255)                                                                      tACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGgtcgtc                                                         >  1:499522/1‑140 (MQ=255)                                                                          ggCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAggg                                                     >  1:391934/1‑140 (MQ=255)                                                                              gcaAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGaaaa                                                 >  1:290870/1‑140 (MQ=255)                                                                               caAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAag                                                <  1:1087176/140‑1 (MQ=255)                                                                                 aaTACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAagag                                              <  1:798536/140‑1 (MQ=255)                                                                                 aaTACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAagag                                              <  1:703059/140‑1 (MQ=255)                                                                                 aaTACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAagag                                              <  2:549567/140‑1 (MQ=255)                                                                                 aaTACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAagag                                              <  1:1261309/140‑1 (MQ=255)                                                                                  atatggTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAgg                                             >  1:945624/5‑140 (MQ=255)                                                                                  aTACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAgg                                             >  1:1181546/1‑140 (MQ=255)                                                                                         tATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTta                                      >  2:226105/1‑140 (MQ=255)                                                                                          aTCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAagag                                              <  2:315591/131‑1 (MQ=255)                                                                                          aTCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAagag                                              >  1:315591/1‑131 (MQ=255)                                                                                          aTCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTtat                                     >  1:107553/1‑140 (MQ=255)                                                                                            cGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAg                                   <  2:107553/140‑1 (MQ=255)                                                                                             gCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTTGTCAGGGAAAAGAGGCGCTTTATAgc                                  <  1:100201/140‑1 (MQ=255)                                                                                             gCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAgc                                  >  2:408803/1‑140 (MQ=255)                                                                                             gCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAgc                                  <  1:854879/140‑1 (MQ=255)                                                                                             gCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAgc                                  <  1:444689/140‑1 (MQ=255)                                                                                              cAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAgcg                                 <  1:855579/140‑1 (MQ=255)                                                                                                gggATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCa                               >  1:994831/1‑140 (MQ=255)                                                                                                  gATGAAAAAATGCGAATTTTACTGATAGAAGATGGCATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCATCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCAcc                             <  2:120155/140‑1 (MQ=255)                                                                                                      aaaaaaTGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTat                         >  2:681312/1‑140 (MQ=255)                                                                                                             gCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCgg                  <  2:499522/140‑1 (MQ=255)                                                                                                              cGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGt                 <  1:408803/140‑1 (MQ=255)                                                                                                                   tttACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATcc            <  2:246625/140‑1 (MQ=255)                                                                                                                        tGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGAt       <  1:640019/140‑1 (MQ=255)                                                                                                                             gaagaTGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAAc  <  1:584100/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                 AGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAAC  >  NC_000913/3169722‑3169985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑