Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,175,678	T→C	Y110C (TAC→TGC)	yqiA ←	esterase YqiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,175,678	0	T	C	100.0%	274.5 / NA	74	Y110C (TAC→TGC)	yqiA	esterase YqiA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/38);  total (36/38)

CGCCTGGCGGTAATCCAGCACTTCATCTCCCGTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACA  >  NC_000913/3175542‑3175812                                                                                                                                         |                                                                                                                                       cGCCTGGCGGTAATCCAGCACTTCATCTCCCGTCTGTTGCAGCAGCCAGATCACATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAgg                                                                                                                                     <  2:1115564/140‑1 (MQ=255) cGCCTGGCGGTAATCCAGCACTTCATCTCCCGTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAgg                                                                                                                                     <  1:345288/140‑1 (MQ=255) cGCCTGGCGGTAATCCAGCACTTCATCTCCCGTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAgg                                                                                                                                     >  1:158158/1‑140 (MQ=255)      ggCGGTAATCCAGCACTTCATCTCCCGTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTtc                                                                                                                                <  1:284148/140‑1 (MQ=255)       gCGGTAATCCAGCACTTCATCTCCCGTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTtct                                                                                                                               >  2:816319/1‑140 (MQ=255)           tAATCCAGCACTTCATCTCCCGTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGtt                                                                                                                           <  1:541623/140‑1 (MQ=255)             aTCCAGCACTTCATCTCCCGTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCt                                                                                                                         <  2:47978/140‑1 (MQ=255)             aTCCAGCACTTCATCTCCCGTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCt                                                                                                                         <  1:145524/140‑1 (MQ=255)             aTCCAGCACTTCATCTCCCGTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGGTCTCGTTCt                                                                                                                         >  2:145524/1‑140 (MQ=255)                cAGCACTTCATCTCCCGTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTTTGAc                                                                                                                      <  2:1266231/140‑1 (MQ=255)                      ttCATCTCCCGTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATTACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGAt                                                                                                                <  2:1136295/140‑1 (MQ=255)                        cATCTCCCGTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAg                                                                                                              >  1:142865/1‑140 (MQ=255)                        cATCTCCCGTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAg                                                                                                              >  2:368789/1‑140 (MQ=255)                         aTCTCCCGTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGt                                                                                                             >  2:664069/1‑140 (MQ=255)                           ctcCCGTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTcc                                                                                                           <  2:424673/140‑1 (MQ=255)                               cGTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTca                                                                                                       >  2:719257/1‑140 (MQ=255)                                gTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTcag                                                                                                      >  2:9937/1‑140 (MQ=255)                                gTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTcag                                                                                                      >  2:227731/1‑140 (MQ=255)                                gTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTcag                                                                                                      >  2:899345/1‑140 (MQ=255)                                     ttGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGtt                                                                                                 <  1:816319/140‑1 (MQ=255)                                      tGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTc                                                                                                >  2:1224227/1‑140 (MQ=255)                                       gcagcaGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCa                                                                                               <  2:1191542/140‑1 (MQ=255)                                       gcagcaGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCa                                                                                               <  2:358207/140‑1 (MQ=255)                                               cAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGgcg                                                                                       <  1:575283/140‑1 (MQ=255)                                                 gATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCa                                                                                     <  1:798185/140‑1 (MQ=255)                                                   tCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCAcc                                                                                   <  1:9937/140‑1 (MQ=255)                                                       aTCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGgcg                                                                                       <  1:1020113/132‑1 (MQ=255)                                                       aTCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGgcg                                                                                       >  2:1020113/1‑132 (MQ=255)                                                       aTCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCAccgccg                                                                               <  2:335524/140‑1 (MQ=255)                                                       aTCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCAccgccg                                                                               <  1:568584/140‑1 (MQ=255)                                                         ccGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCggg                                                                             <  1:719257/140‑1 (MQ=255)                                                         ccGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCggg                                                                             <  1:338038/140‑1 (MQ=255)                                                         ccGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCggg                                                                             <  1:118318/140‑1 (MQ=255)                                                                     gCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACt                                                                 <  2:142865/140‑1 (MQ=255)                                                                          tCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCggg                                                            <  1:123751/140‑1 (MQ=255)                                                                          tCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCggg                                                            <  2:207698/140‑1 (MQ=255)                                                                            aaTCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGgca                                                          >  1:741763/1‑140 (MQ=255)                                                                              tCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGgcagc                                                        <  2:158158/140‑1 (MQ=255)                                                                                  cATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATaa                                                    <  1:1154999/140‑1 (MQ=255)                                                                                  cATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATaa                                                    <  1:209173/140‑1 (MQ=255)                                                                                   aTGACTTTAAGATCGTAAATATGGCGTGACTCCAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATaaa                                                   >  1:447778/1‑140 (MQ=255)                                                                                      aCTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACa                                                <  2:301022/140‑1 (MQ=255)                                                                                       cTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACAt                                               >  1:130929/1‑140 (MQ=255)                                                                                        tttAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACAtt                                              >  2:392921/1‑140 (MQ=255)                                                                                        tttAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACAtt                                              >  1:298064/1‑140 (MQ=255)                                                                                          tAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATtgt                                            >  2:1213769/1‑140 (MQ=255)                                                                                           aaGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATtgtg                                           <  1:318158/140‑1 (MQ=255)                                                                                                 gTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAAcc                                     <  1:705329/140‑1 (MQ=255)                                                                                                 gTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAAcc                                     >  2:431665/1‑140 (MQ=255)                                                                                                 gTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAAcc                                     <  2:527311/140‑1 (MQ=255)                                                                                                 gTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAAcc                                     >  2:96036/1‑140 (MQ=255)                                                                                                 gTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAAcc                                     >  1:527311/1‑140 (MQ=255)                                                                                                  tAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCa                                    >  1:225627/1‑140 (MQ=255)                                                                                                  tAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCa                                    >  1:321687/1‑140 (MQ=255)                                                                                                         ggagTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCg                             >  1:955989/4‑140 (MQ=255)                                                                                                         ggCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCg                             >  2:795229/1‑140 (MQ=255)                                                                                                          gCGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGt                            >  1:1159921/1‑140 (MQ=255)                                                                                                           cGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAg                                   >  1:604148/1‑132 (MQ=255)                                                                                                           cGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAg                                   <  2:604148/132‑1 (MQ=255)                                                                                                           cGTGACTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTa                           >  1:649060/1‑140 (MQ=255)                                                                                                               aCTCTAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAatat                       <  2:266439/140‑1 (MQ=255)                                                                                                                ctctAGCACATATTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATc                      <  1:472202/140‑1 (MQ=255)                                                                                                                           atTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCg                             >  1:110814/1‑122 (MQ=255)                                                                                                                           atTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCg                             <  2:110814/122‑1 (MQ=255)                                                                                                                           atTGCTGCCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGAc           >  1:501189/1‑140 (MQ=255)                                                                                                                             tgctgcCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGa         <  2:321687/140‑1 (MQ=255)                                                                                                                             tgctgcCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGa         <  2:955989/140‑1 (MQ=255)                                                                                                                               ctgcCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAAc       >  1:1220925/1‑140 (MQ=255)                                                                                                                               ctgcCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAAc       >  2:1265743/1‑140 (MQ=255)                                                                                                                                 gcCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAAc       >  2:292314/1‑138 (MQ=255)                                                                                                                                 gcCCGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAAc       <  1:292314/138‑1 (MQ=255)                                                                                                                                   ccGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGAc   >  2:191853/1‑140 (MQ=255)                                                                                                                                    cGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACa  >  1:799334/1‑140 (MQ=255)                                                                                                                                    cGGTGCAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACa  >  2:1242617/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CGCCTGGCGGTAATCCAGCACTTCATCTCCCGTCTGTTGCAGCAGCCAGATCAAATCCGGCGCTTCCAGCGGGTCAATCTGCATGACTTTAAGATCGTAAATATGGCGTGACTCTAGCACATATTGCTGCCCGGTGTAGGGGTTCTCGTTCTGACCGAGATAGTCCGTCAGCAGTTCAAACGGGCGCACCGCCGGGTTTACCACCACTGCGGGCAGCATAAAACATTGTGACAACCAGGTGGCGTAATATCCCCCCAGTGACGAACCGACA  >  NC_000913/3175542‑3175812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑