Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,177,351	T→C	N187S (AAC→AGC)	nudF ←	ADP‑sugar pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,177,351	0	T	C	100.0%	234.3 / NA	63	N187S (AAC→AGC)	nudF	ADP‑sugar pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/40);  total (23/40)

TTGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGTTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCC  >  NC_000913/3177218‑3177475                                                                                                                                      |                                                                                                                             ttGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGa                                                                                                                        >  1:1270777/1‑140 (MQ=255)   gtgAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGAtt                                                                                                                      <  2:1072249/140‑1 (MQ=255)    tgAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGAttt                                                                                                                     <  1:65226/140‑1 (MQ=255)      aaaaGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTc                                                                                                                   <  1:839231/140‑1 (MQ=255)        aaGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTccc                                                                                                                 <  1:1046244/140‑1 (MQ=255)        aaGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTccc                                                                                                                 <  2:869163/140‑1 (MQ=255)        aaGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTccc                                                                                                                 <  2:62780/140‑1 (MQ=255)        aaGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTccc                                                                                                                 <  2:392741/140‑1 (MQ=255)          gTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCct                                                                                                               <  1:943446/140‑1 (MQ=255)          gTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCct                                                                                                               <  2:742993/140‑1 (MQ=255)           ttCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTccc                                                                                                                 >  1:1269670/1‑137 (MQ=255)           ttCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTccc                                                                                                                 <  2:1269670/137‑1 (MQ=255)             cATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCtctt                                                                                                            <  1:928822/140‑1 (MQ=255)             cATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCtctt                                                                                                            <  2:443930/140‑1 (MQ=255)               tctcGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCtcttct                                                                                                          <  1:1067861/140‑1 (MQ=255)                ctcGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTa                                                                                                         <  2:199938/140‑1 (MQ=255)                   gCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTAccc                                                                                                      <  1:500686/140‑1 (MQ=255)                   gCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTAccc                                                                                                      <  2:1163303/140‑1 (MQ=255)                    cacaGGCGCACCATCTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCa                                                                                                     <  2:1108716/140‑1 (MQ=255)                     acaGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTc                                                                                                                   <  1:500849/125‑1 (MQ=255)                     acaGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTc                                                                                                                   >  2:500849/1‑125 (MQ=255)                     acaGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCAc                                                                                                    >  1:344643/1‑140 (MQ=255)                          cgcATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTAccc                                                                                                      >  2:1192269/1‑133 (MQ=255)                          cgcATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTAccc                                                                                                      <  1:1192269/133‑1 (MQ=255)                             atcatTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGc                                                                                            >  2:1228102/1‑140 (MQ=255)                             atcatTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGc                                                                                            <  2:1088674/140‑1 (MQ=255)                              tcatTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTAccc                                                                                                      >  2:837652/1‑129 (MQ=255)                              tcatTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTAccc                                                                                                      <  1:837652/129‑1 (MQ=255)                                   tCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTc                                                                                      >  2:952327/1‑140 (MQ=255)                                    cAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTcc                                                                                     <  2:344643/140‑1 (MQ=255)                                       gAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGTCCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCgg                                                                                  <  1:365020/140‑1 (MQ=255)                                       gAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCgg                                                                                  <  2:623276/140‑1 (MQ=255)                                         aaGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCt                                                                                <  2:236477/140‑1 (MQ=255)                                         aaGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCt                                                                                <  1:647771/140‑1 (MQ=255)                                            tCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTAc                                                                             <  2:97172/140‑1 (MQ=255)                                                 tgtgtAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTT‑CCGGCTTACCACATg                                                                       <  1:952327/140‑1 (MQ=255)                                                   tgtAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGa                                                                      <  2:386443/140‑1 (MQ=255)                                                      aaCGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAAcg                                                                   >  1:896970/1‑140 (MQ=255)                                                          cTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGaa                                                               >  2:227117/1‑140 (MQ=255)                                                           ttCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAat                                                              >  1:814008/1‑140 (MQ=255)                                                             cATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAatat                                                            >  2:72860/1‑140 (MQ=255)                                                             cATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAatat                                                            >  2:501501/1‑140 (MQ=255)                                                             cATTTATGCCCACTCATTTTTCAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGA‑TTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATc                                                           >  2:999137/1‑140 (MQ=255)                                                                     cccACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGttt                                                    >  2:162122/1‑140 (MQ=255)                                                                     cccACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGttt                                                    >  1:89620/1‑140 (MQ=255)                                                                        aCTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTtca                                                 >  2:1064997/1‑140 (MQ=255)                                                                         cTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTtcat                                                <  1:1228102/140‑1 (MQ=255)                                                                         cTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTtcat                                                >  1:325848/1‑140 (MQ=255)                                                                         cTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTtcat                                                <  1:162122/140‑1 (MQ=255)                                                                             ttttttAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGc                                            <  2:814008/140‑1 (MQ=255)                                                                              tttttAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGcc                                           <  2:709760/140‑1 (MQ=255)                                                                              tttttAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGACTTACCACATGAACGCGAATATCTTCGTTTTCATCAGcc                                           <  1:102114/140‑1 (MQ=255)                                                                                tttAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGGCGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAg                                         <  2:1218289/140‑1 (MQ=255)                                                                                tttAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAg                                         <  1:713506/140‑1 (MQ=255)                                                                                    aCGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCg                                     >  1:260872/1‑140 (MQ=255)                                                                                        ttGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGaa                                 <  1:546993/140‑1 (MQ=255)                                                                                           atgatgCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGCTGTCGA‑TTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATAcc                             <  1:999137/140‑1 (MQ=255)                                                                                               tgcagctgcagcCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCt                          <  1:876230/140‑1 (MQ=255)                                                                                                gcagctgcagcCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCtt                         >  2:230620/1‑140 (MQ=255)                                                                                                    ctgcagcCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCgg                     >  2:1061673/1‑140 (MQ=255)                                                                                                      gcagcCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTc                   <  2:325848/140‑1 (MQ=255)                                                                                                       cagcCATTGCAAAGCGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCg                  >  2:81724/1‑140 (MQ=255)                                                                                                                     cGATGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCg    <  1:501501/140‑1 (MQ=255)                                                                                                                       aTGACCGACGCTGCGCTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGcc  >  2:267211/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                             TTGTGAAAAGTTCATCTCGCACAGGCGCATCATTTCAGGAAAGTCAGGTGTGTAACGCTTCATTTATGCCCACTCATTTTTTAACGCTTGATGATGCAGCTGCAGCCATTGCAAAGCGATGACCGACGCTGCGTTGTCGATTTTCCCCTCTTCTACCCACTGGTATGCCTGTTCCCGGCTTACCACATGAACGCGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCC  >  NC_000913/3177218‑3177475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑