Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,178,848	T→C	L245P (CTG→CCG)	tolC →	outer membrane channel TolC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,178,848	0	T	C	100.0%	308.2 / NA	82	L245P (CTG→CCG)	tolC	outer membrane channel TolC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/48);  total (34/48)

CGCGGTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTTATAGCGGTTCGAAAACCCGTGGT  >  NC_000913/3178711‑3178984                                                                                                                                          |                                                                                                                                           cgcgGTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGt                                                                                                                                          >  1:1086819/1‑140 (MQ=255)   cgGTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTa                                                                                                                                        <  2:207124/140‑1 (MQ=255)    ggTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTAc                                                                                                                                       <  1:883840/140‑1 (MQ=255)    ggTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTAc                                                                                                                                       <  1:797019/140‑1 (MQ=255)    ggTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTAc                                                                                                                                       >  2:630733/1‑140 (MQ=255)      tAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAg                                                                                                                                     >  1:150708/1‑140 (MQ=255)             gcTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCt                                                                                                                              <  1:205712/140‑1 (MQ=255)             gcTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCt                                                                                                                              <  1:673676/140‑1 (MQ=255)              cTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGc                                                                                                                               <  2:253475/138‑1 (MQ=255)              cTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGc                                                                                                                               >  1:253475/1‑138 (MQ=255)                gcgcCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGGCAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGa                                                                                                                           >  2:966847/1‑140 (MQ=255)                gcgcCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGa                                                                                                                           >  1:519307/1‑140 (MQ=255)                   ccAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGcc                                                                                                                        <  1:984335/140‑1 (MQ=255)                   ccAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGcc                                                                                                                        <  1:763207/140‑1 (MQ=255)                       aTCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGa                                                                                                                    >  2:1244418/1‑140 (MQ=255)                              gTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCtt                                                                                                                             >  2:294803/1‑124 (MQ=255)                              gTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCtt                                                                                                                             <  1:294803/124‑1 (MQ=255)                                aaCTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGgcgcg                                                                                                           <  2:281451/140‑1 (MQ=255)                                         ccGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAAt                                                                                                  >  1:395355/1‑140 (MQ=255)                                         ccGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAAt                                                                                                  <  1:667909/140‑1 (MQ=255)                                         ccGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAAt                                                                                                  >  2:371290/1‑140 (MQ=255)                                         ccGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAAt                                                                                                  >  1:254892/1‑140 (MQ=255)                                             aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              >  2:21464/1‑140 (MQ=255)                                             aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              >  1:614866/1‑140 (MQ=255)                                             aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              <  2:614866/140‑1 (MQ=255)                                             aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              >  1:493187/1‑140 (MQ=255)                                             aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              >  2:646663/1‑140 (MQ=255)                                             aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              >  2:1250713/1‑140 (MQ=255)                                             aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              >  2:845447/1‑140 (MQ=255)                                             aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              <  1:193264/140‑1 (MQ=255)                                             aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              >  1:1192701/1‑140 (MQ=255)                                             aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              >  2:923688/1‑140 (MQ=255)                                             aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              <  1:1061531/140‑1 (MQ=255)                                             aaCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGc                                                                                              <  1:827175/140‑1 (MQ=255)                                              aCTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGcc                                                                                             <  1:89881/140‑1 (MQ=255)                                                 ggCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAgg                                                                                          <  1:1188117/140‑1 (MQ=255)                                                  gCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGgc                                                                                         >  2:927972/1‑140 (MQ=255)                                                  gCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGgc                                                                                         >  1:866866/1‑140 (MQ=255)                                                  gCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGgc                                                                                         >  1:71116/1‑140 (MQ=255)                                                  gCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGgc                                                                                         >  2:627723/1‑140 (MQ=255)                                                  gCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGgc                                                                                         >  1:1049364/1‑140 (MQ=255)                                                   cTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGgcg                                                                                        <  2:81149/140‑1 (MQ=255)                                                    tGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGgcgc                                                                                       <  2:235972/140‑1 (MQ=255)                                                    tGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGgcgc                                                                                       <  2:232737/140‑1 (MQ=255)                                                        cTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGa                                                                                   <  2:150708/140‑1 (MQ=255)                                                        cTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGa                                                                                   <  2:716641/140‑1 (MQ=255)                                                        cTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGa                                                                                   <  1:927972/140‑1 (MQ=255)                                                        cTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGa                                                                                   <  1:1199331/140‑1 (MQ=255)                                                            aTGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGt                                                                               <  2:571434/140‑1 (MQ=255)                                                                cGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCGCt                                                                           >  2:785141/1‑140 (MQ=255)                                                                       tttAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGAc                                                                    >  1:952839/1‑140 (MQ=255)                                                                       tttAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGAc                                                                    >  2:313699/1‑140 (MQ=255)                                                                        ttAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGAct                                                                   <  2:1049364/140‑1 (MQ=255)                                                                                 aCAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTa                                                          >  2:915750/1‑140 (MQ=255)                                                                                 aCAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTa                                                          <  1:209136/140‑1 (MQ=255)                                                                                      ccACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGc                                                     <  2:569142/140‑1 (MQ=255)                                                                                      ccACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGc                                                     <  1:785141/140‑1 (MQ=255)                                                                                      ccACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGc                                                     <  1:630733/140‑1 (MQ=255)                                                                                          aGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCt                                                 >  2:345385/1‑140 (MQ=255)                                                                                            ccGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTAc                                               <  2:687083/140‑1 (MQ=255)                                                                                               gTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCgg                                            >  2:756217/1‑140 (MQ=255)                                                                                                ttAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCggg                                           <  2:703053/140‑1 (MQ=255)                                                                                                 tAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGa                                          >  1:490766/1‑140 (MQ=255)                                                                                                 tAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGa                                          <  1:966847/140‑1 (MQ=255)                                                                                                         tgctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGac                                  <  2:71116/140‑1 (MQ=255)                                                                                                         tgctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGac                                  <  1:1244418/140‑1 (MQ=255)                                                                                                         tgctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGac                                  <  1:1057465/140‑1 (MQ=255)                                                                                                         tgctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGac                                  <  1:59778/140‑1 (MQ=255)                                                                                                         tgctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGac                                  <  1:646663/140‑1 (MQ=255)                                                                                                         tgctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGac                                  <  1:915750/140‑1 (MQ=255)                                                                                                         tgctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGac                                  <  2:17269/140‑1 (MQ=255)                                                                                                         tgctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGac                                  <  2:1192701/140‑1 (MQ=255)                                                                                                          gctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGaca                                 <  1:313699/140‑1 (MQ=255)                                                                                                          gctgAAAGAAGCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGaca                                 <  2:952839/140‑1 (MQ=255)                                                                                                                   agCCGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTtata                      >  1:83733/1‑140 (MQ=255)                                                                                                                     ccGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTTATAGc                    >  1:821206/1‑140 (MQ=255)                                                                                                                     ccGAAAAACGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTTATAGc                    >  2:1065433/1‑140 (MQ=255)                                                                                                                             cGCAACCTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTTATAGCGGTTCGaa            <  1:627723/140‑1 (MQ=255)                                                                                                                                   cTGTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTTATAGCGGTTCGAAAACCCg      <  1:425873/140‑1 (MQ=255)                                                                                                                                     gTCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACACCTCTCTTATAGCGGTTCGAAAACCCg      <  2:490766/140‑1 (MQ=255)                                                                                                                                      tCGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTCATAGCGGTTCGAAAACCCgtgg   >  1:820102/1‑140 (MQ=255)                                                                                                                                       cGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTTATAGCGGTTCGAAAACCCgtggt  <  2:1086819/140‑1 (MQ=255)                                                                                                                                       cGCCGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTTATAGCGGTTCGAAAACCCg      <  1:451726/136‑1 (MQ=255)                                                                                                                                          |                                                                                                                                           CGCGGTAGAGCAGCTGCGCCAGATCACCGGTAACTACTATCCGGAACTGGCTGCGCTGAATGTCGAAAACTTTAAAACCGACAAACCACAGCCGGTTAACGCGCTGCTGAAAGAAGCCGAAAAACGCAACCTGTCGCTGTTACAGGCACGCTTGAGCCAGGACCTGGCGCGCGAGCAAATTCGCCAGGCGCAGGATGGTCACTTACCGACTCTGGATTTAACGGCTTCTACCGGGATTTCTGACAC‑‑CTCTTATAGCGGTTCGAAAACCCGTGGT  >  NC_000913/3178711‑3178984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑