Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	352,394	G→A	D394N (GAC→AAC)	prpD →	2‑methylcitrate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	352,394	0	G	A	100.0%	293.2 / NA	84	D394N (GAC→AAC)	prpD	2‑methylcitrate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/46);  total (38/46)

AGTACATGGTGGCGATCCCGCTGCTATTCGGGCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTGACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTGGTCATGCTCGCCGCCGTCAGGATGGTATTCCGAAA  >  NC_000913/352260‑352528                                                                                                                                       |                                                                                                                                       aGTACATGGTGGCGATCCCGCTGCTATTCGGGCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTAc                                                                                                                                   >  2:1068350/1‑140 (MQ=255) aGTACATGGTGGCGATCCCGCTGCTATTCGGGCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTAc                                                                                                                                   >  2:761963/1‑140 (MQ=255)  gTACATGGTGGCGATCCCGCTGCTATTCGGGCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGGGCGAGGAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTAcc                                                                                                                                  >  2:597756/1‑140 (MQ=255)  gTACATGGTGGCGATCCCGCTGCTATTCGGGCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTAcc                                                                                                                                  >  1:1157225/1‑140 (MQ=255)   tACATGGTGGCGATCCCGCTGCTATTCGGGCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCa                                                                                                                                 <  1:761963/140‑1 (MQ=255)    aCATGGTGGCGATCCCGCTGCTATTCGGGCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCAc                                                                                                                                >  1:685973/1‑140 (MQ=255)        ggtggCGATCCCGCTGCTATTCGGGCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAacgaccacgacc                                                                                                                            >  2:1128112/1‑140 (MQ=255)        ggtggCGATCCCGCTGCTATTCGGGCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGAcc                                                                                                                            >  1:66398/1‑140 (MQ=255)           ggCGATCCCGCTGCTATTCGGGCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCgg                                                                                                                         >  2:344849/1‑140 (MQ=255)             cGATCCCGCTGCTATTCGGGCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGaa                                                                                                                       >  2:465062/1‑140 (MQ=255)             cGATCCCGCTGCTATTCGGGCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGaa                                                                                                                       >  2:325360/1‑140 (MQ=255)               aTCCCGCTGCTATTCGGGCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGaaaa                                                                                                                     >  2:1209331/1‑140 (MQ=255)                    gctgctATTCGGGCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAAcgcg                                                                                                                >  1:1217375/1‑140 (MQ=255)                    gctgctATTCGGGCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAAcgcg                                                                                                                >  2:1102597/1‑140 (MQ=255)                      tgctATTCGGGCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGcc                                                                                                              <  2:760160/140‑1 (MQ=255)                       gctATTCGGGCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCa                                                                                                             <  2:858001/140‑1 (MQ=255)                             cGGGCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCa                                                                                                       <  1:1203620/140‑1 (MQ=255)                              gggCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCaa                                                                                                      <  1:708718/140‑1 (MQ=255)                              gggCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCaa                                                                                                      <  1:1040354/140‑1 (MQ=255)                                 cgcTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGc                                                                                                   <  1:1068350/140‑1 (MQ=255)                                   cTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCa                                                                                                 >  2:1105716/1‑140 (MQ=255)                                   cTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCa                                                                                                 >  2:677171/1‑140 (MQ=255)                                   cTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCa                                                                                                 >  2:788650/1‑140 (MQ=255)                                   cTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCa                                                                                                 <  1:774853/140‑1 (MQ=255)                                   cTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCa                                                                                                 >  1:658110/1‑140 (MQ=255)                                   cTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCa                                                                                                 >  1:367898/1‑140 (MQ=255)                                    ttAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCAt                                                                                                <  2:685973/140‑1 (MQ=255)                                    ttAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCAt                                                                                                <  1:5842/140‑1 (MQ=255)                                           cggcAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCtt                                                                                         <  2:367898/140‑1 (MQ=255)                                           cggcAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCtt                                                                                         <  1:325360/140‑1 (MQ=255)                                            ggcAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTg                                                                                        <  1:152776/140‑1 (MQ=255)                                              cAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAg                                                                                      >  1:590268/1‑140 (MQ=255)                                                    aCGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCAcc                                                                                <  1:415197/140‑1 (MQ=255)                                                    aCGAGGACAACGTTGCGCAAGATAAACGCATTGACGACCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCAcc                                                                                <  1:520229/140‑1 (MQ=255)                                                          aCAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGc                                                                          <  2:66398/140‑1 (MQ=255)                                                           cAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGca                                                                         >  1:600095/1‑140 (MQ=255)                                                           cAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGca                                                                         >  1:741726/1‑140 (MQ=255)                                                            aaCGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGcac                                                                        <  2:757885/140‑1 (MQ=255)                                                             aCGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGcaca                                                                       <  2:1187022/140‑1 (MQ=255)                                                             aCGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGcaca                                                                       <  2:600095/140‑1 (MQ=255)                                                              cGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCacac                                                                      >  2:676735/1‑140 (MQ=255)                                                               gTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACg                                                                     <  1:1102597/140‑1 (MQ=255)                                                               gTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACg                                                                     <  1:1128112/140‑1 (MQ=255)                                                                ttGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGa                                                                    >  1:545717/1‑140 (MQ=255)                                                                 tGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGAt                                                                   >  1:1030369/1‑140 (MQ=255)                                                                      aaGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTgaa                                                              <  2:721722/140‑1 (MQ=255)                                                                        gATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTgaaga                                                            <  1:1209331/140‑1 (MQ=255)                                                                         aTAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTgaagaa                                                           >  2:1170020/1‑140 (MQ=255)                                                                         aTAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTgaagaa                                                           >  1:1242478/1‑140 (MQ=255)                                                                          tAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGaagaag                                                          <  1:676735/140‑1 (MQ=255)                                                                          tAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGaagaag                                                          <  2:292450/140‑1 (MQ=255)                                                                             aCGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTGCCCTTGAGTTCACCGACGGCACACGATTTGAAGAAgtgg                                                       <  1:344849/140‑1 (MQ=255)                                                                               gCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAgtggtg                                                     <  1:66582/140‑1 (MQ=255)                                                                                cATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAgtggtgg                                                    <  1:1105716/140‑1 (MQ=255)                                                                                cATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAgtggtgg                                                    <  2:1217375/140‑1 (MQ=255)                                                                                  ttGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAgtggtggtg                                                  <  2:741726/140‑1 (MQ=255)                                                                                  ttGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAgtggtggtg                                                  <  2:545717/140‑1 (MQ=255)                                                                                  ttGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAgtggtggtg                                                  <  2:344368/140‑1 (MQ=255)                                                                                    gACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGa                                                <  1:5224/140‑1 (MQ=255)                                                                                     aCGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAg                                               <  2:1157225/140‑1 (MQ=255)                                                                                         ccTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGGGGTGGAGTAcc                                           >  2:636819/1‑140 (MQ=255)                                                                                          cTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTAccc                                          >  1:766799/1‑140 (MQ=255)                                                                                            gcgcGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCa                                        <  2:1030369/140‑1 (MQ=255)                                                                                             cgcgAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAgtggtgg                                                    <  1:544138/127‑1 (MQ=255)                                                                                             cgcgAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAgtggtgg                                                    >  2:544138/1‑127 (MQ=255)                                                                                                gagaAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTgg                                    <  2:1242478/140‑1 (MQ=255)                                                                                                   aaggTCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTGGTCa                                 >  2:763361/1‑140 (MQ=255)                                                                                                      aTCAATTGCTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTGGTCATGc                              <  1:529661/140‑1 (MQ=255)                                                                                                              cTTTGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTGGTCATGCTCgccgcc                      >  1:1261262/1‑140 (MQ=255)                                                                                                                ttGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTGGTCATGCTCGCCGCCGt                    >  1:580020/1‑140 (MQ=255)                                                                                                                ttGAAGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTGGTCATGCTCGCCGCCGt                    >  1:453812/1‑140 (MQ=255)                                                                                                                   aaGATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTGGTCATGCTCGCCGCCGTCAg                 <  2:865815/140‑1 (MQ=255)                                                                                                                     gATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTATCCTTCAAGCCGACGACGGCATACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTGGTCATGCTCGCCGCCGTCAGGa               <  1:234392/140‑1 (MQ=255)                                                                                                                     gATCCGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTGGTCATGCTCGCCGCCGTCAGGa               <  2:1020751/140‑1 (MQ=255)                                                                                                                        ccGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTGGTCATGCTCGCCGCCGTCa                  <  1:804979/134‑1 (MQ=255)                                                                                                                        ccGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTGGTCATGCTCGCCGCCGTCa                  >  2:804979/1‑134 (MQ=255)                                                                                                                        ccGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTGGTCATGCTCGCCGCCGTCAGGATgg            >  1:32691/1‑140 (MQ=255)                                                                                                                        ccGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTGGTCATGCTCGCCGCCGTCAGGATgg            >  1:538966/1‑140 (MQ=255)                                                                                                                        ccGGCATTTACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGGGGTGGAGTACCCCATTGGTCATGCTCGCCGCCGTCAGGATgg            >  2:1180149/1‑140 (MQ=255)                                                                                                                                tACCGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTGGTCATGCTCGCCGCCGTCAGGATGGTATTCCGa    <  1:597756/140‑1 (MQ=255)                                                                                                                                  ccGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTGGTCATGCTCGCCGCCGTCAGGATGGTATTCCGaaa  <  1:465062/140‑1 (MQ=255)                                                                                                                                  ccGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTGGTCATGCTCGCCGCCGTCAGGATGGTATTCCGaaa  <  2:1261262/140‑1 (MQ=255)                                                                                                                                   cGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTGGTCATGCTCGCCGCCGTCa                  >  2:105443/1‑123 (MQ=255)                                                                                                                                   cGCTAACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTGGTCATGCTCGCCGCCGTCa                  <  1:105443/123‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       AGTACATGGTGGCGATCCCGCTGCTATTCGGGCGCTTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGCGAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTGACTACCACGACCCGGAAAAACGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTGGTGGTGGAGTACCCCATTGGTCATGCTCGCCGCCGTCAGGATGGTATTCCGAAA  >  NC_000913/352260‑352528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑