Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,307,964	T→C	intergenic (‑31/+76)	yrbN ← / ← nlpI	uncharacterized protein YrbN/lipoprotein NlpI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,307,964	0	T	C	100.0%	310.9 / NA	84	intergenic (‑31/+76)	yrbN/nlpI	uncharacterized protein YrbN/lipoprotein NlpI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/44);  total (40/44)

GATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATT  >  NC_000913/3307830‑3308095                                                                                                                                       |                                                                                                                                    gATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTaa                                                                                                                                >  2:901224/1‑140 (MQ=255)      gCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGaac                                                                                                                           >  2:1030932/1‑140 (MQ=255)      gCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGaac                                                                                                                           >  2:1169835/1‑140 (MQ=255)      gCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGaac                                                                                                                           >  2:179017/1‑140 (MQ=255)          aaGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGaacaaca                                                                                                                       <  2:359434/140‑1 (MQ=255)            gTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGaacaacaac                                                                                                                     >  2:1211160/1‑140 (MQ=255)            gTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGaacaacaac                                                                                                                     >  2:874757/1‑140 (MQ=255)             tGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACg                                                                                                                    <  2:207982/140‑1 (MQ=255)                  tCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTc                                                                                                               <  1:1030932/140‑1 (MQ=255)                  tCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTc                                                                                                               <  1:1169835/140‑1 (MQ=255)                    gAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCAc                                                                                                             <  1:901224/140‑1 (MQ=255)                         cAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGtt                                                                                                        <  2:909047/140‑1 (MQ=255)                          aGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTa                                                                                                       <  1:226117/140‑1 (MQ=255)                               tGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTg                                                                                                  >  1:1025814/1‑140 (MQ=255)                                      cGTGTGCCTCAAAATTAATGGCGGCCAGTCTATATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCaa                                                                                           <  2:248929/140‑1 (MQ=255)                                      cGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCaa                                                                                           >  2:88316/1‑140 (MQ=255)                                      cGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCaa                                                                                           >  2:960503/1‑140 (MQ=255)                                         gtgCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCaa                                                                                           >  1:1162033/1‑137 (MQ=255)                                         gtgCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCaa                                                                                           <  2:1162033/137‑1 (MQ=255)                                         gtgCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCa                                                                                        >  2:7491/1‑140 (MQ=255)                                         gtgCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCa                                                                                        >  2:718893/1‑140 (MQ=255)                                          tgCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCaa                                                                                       >  2:967765/1‑140 (MQ=255)                                          tgCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCaa                                                                                       >  2:1055164/1‑140 (MQ=255)                                          tgCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCaa                                                                                       >  1:993429/1‑140 (MQ=255)                                          tgCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCaa                                                                                       >  1:7931/1‑140 (MQ=255)                                           gCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCaaa                                                                                      >  2:330656/1‑140 (MQ=255)                                           gCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCaaa                                                                                      >  2:387171/1‑140 (MQ=255)                                           gCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCaaa                                                                                      >  1:1158521/1‑140 (MQ=255)                                           gCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCaaa                                                                                      >  2:686391/1‑140 (MQ=255)                                            ccTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCaaaa                                                                                     >  1:157096/1‑140 (MQ=255)                                            ccTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCaaaa                                                                                     >  2:7920/1‑140 (MQ=255)                                              tCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCaaaaaa                                                                                   >  2:125727/1‑140 (MQ=255)                                              tCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCaaaaaa                                                                                   >  2:7975/1‑140 (MQ=255)                                                  aaTTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGAtt                                                                               <  1:144988/140‑1 (MQ=255)                                                  aaTTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGAtt                                                                               >  2:1225504/1‑140 (MQ=255)                                                  aaTTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGAtt                                                                               <  1:387171/140‑1 (MQ=255)                                                      aaTGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACgg                                                                           >  2:707330/1‑140 (MQ=255)                                                       aTGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACggg                                                                          <  1:967765/140‑1 (MQ=255)                                                       aTGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACggg                                                                          <  1:179017/140‑1 (MQ=255)                                                       aTGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACggg                                                                          <  2:157096/140‑1 (MQ=255)                                                       aTGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACggg                                                                          <  1:1225504/140‑1 (MQ=255)                                                       aTGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACggg                                                                          <  1:818598/140‑1 (MQ=255)                                                       aTGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACggg                                                                          <  1:7975/140‑1 (MQ=255)                                                        tGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGc                                                                         <  1:88316/140‑1 (MQ=255)                                                         ggcggcCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCt                                                                        <  2:606309/140‑1 (MQ=255)                                                          gcggcCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTg                                                                       <  2:182938/140‑1 (MQ=255)                                                          gcggcCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTg                                                                       <  1:305656/140‑1 (MQ=255)                                                          gcggcCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTg                                                                       <  2:910634/140‑1 (MQ=255)                                                            ggcCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGAt                                                                     <  1:1217706/140‑1 (MQ=255)                                                             gcCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGAtg                                                                    <  1:1142872/140‑1 (MQ=255)                                                                   cTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACg                                                              <  2:7931/140‑1 (MQ=255)                                                                   cTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACg                                                              <  2:993429/140‑1 (MQ=255)                                                                           cTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTAt                                                      >  2:998400/1‑140 (MQ=255)                                                                            tcatcaTGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAGGATTACGGGCTGATGTGTACg                                                              <  1:225726/131‑1 (MQ=255)                                                                            tcatcaTGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAGGATTACGGGCTGATGTGTACg                                                              >  2:225726/1‑131 (MQ=255)                                                                            tcatcaTGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTAtt                                                     >  2:88466/1‑140 (MQ=255)                                                                            tcatcaTGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTAtt                                                     >  2:217421/1‑140 (MQ=255)                                                                              atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGc                                                   >  1:611672/1‑140 (MQ=255)                                                                              atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGc                                                   >  1:525833/1‑140 (MQ=255)                                                                              atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGc                                                   >  1:960333/1‑140 (MQ=255)                                                                                   gAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTc                                              >  1:205744/1‑140 (MQ=255)                                                                                      aTTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGa                                           <  2:479725/140‑1 (MQ=255)                                                                                       ttGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACATCAACTTCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGTCTGATGTGTACGTCAGCTATTGCTGGTCCGAt                                          <  1:668861/140‑1 (MQ=255)                                                                                               cAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAg                                  >  2:398079/1‑140 (MQ=255)                                                                                                     tCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATc                            >  2:906928/1‑140 (MQ=255)                                                                                                      cATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCt                           <  1:33463/140‑1 (MQ=255)                                                                                                      cATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCt                           <  1:1253043/140‑1 (MQ=255)                                                                                                      cATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCt                           <  2:131027/140‑1 (MQ=255)                                                                                                            aaaGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                     <  1:125727/140‑1 (MQ=255)                                                                                                            aaaGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                     <  1:564526/140‑1 (MQ=255)                                                                                                            aaaGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                     <  1:960503/140‑1 (MQ=255)                                                                                                            aaaGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                     <  2:205744/140‑1 (MQ=255)                                                                                                            aaaGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                     <  1:1055164/140‑1 (MQ=255)                                                                                                            aaaGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                     <  1:707330/140‑1 (MQ=255)                                                                                                                tgtgAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGc                 >  2:440273/1‑140 (MQ=255)                                                                                                                     aCCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAgg            >  2:1054123/1‑140 (MQ=255)                                                                                                                         gCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCg        <  1:874757/140‑1 (MQ=255)                                                                                                                         gCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCg        <  1:217421/140‑1 (MQ=255)                                                                                                                          cTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGa       >  1:195107/1‑140 (MQ=255)                                                                                                                          cTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGa       >  1:1212727/1‑140 (MQ=255)                                                                                                                           tCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGAt      <  2:525833/140‑1 (MQ=255)                                                                                                                           tCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGAt      <  1:7491/140‑1 (MQ=255)                                                                                                                           tCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGAt      <  2:86553/140‑1 (MQ=255)                                                                                                                               aGTAGGTGCATTAACGAACAACAACACCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAAtt  <  1:1211160/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    GATCTGCAAAAGTGGTTTCGAATTCAGCCATGTAGTACGTGTGCCTCAAAATTAATGGCGGCCAGTCTACATAACTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATT  >  NC_000913/3307830‑3308095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑