Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,344,150	G→A	L69L (CTG→CTA)	lptB →	lipopolysaccharide transport system ATP binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,344,150	0	G	A	100.0%	200.7 / NA	58	L69L (CTG→CTA)	lptB	lipopolysaccharide transport system ATP binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/26);  total (32/26)

GACCGTCAACTCCGGGGAAATTGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGAACA  >  NC_000913/3344015‑3344281                                                                                                                                        |                                                                                                                                    gACCGTCAACTCCGGGGAAATTGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCtactgc                                                                                                                                 <  2:1129462/140‑1 (MQ=255)             cGGGGAAATTGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTTTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCAc                                                                                                                     >  2:790706/1‑140 (MQ=255)                  aaaTTGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCAcgcgcg                                                                                                                <  2:6226/140‑1 (MQ=255)                  aaaTTGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCAcgcgcg                                                                                                                <  1:1171656/140‑1 (MQ=255)                       gTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGcgccg                                                                                                           <  1:521945/140‑1 (MQ=255)                       gTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGcgccg                                                                                                           <  1:168259/140‑1 (MQ=255)                            tCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTa                                                                                                      >  1:78379/1‑140 (MQ=255)                               gctgGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCg                                                                                                   >  2:209673/1‑140 (MQ=255)                                  ggggCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCt                                                                                                <  2:308800/140‑1 (MQ=255)                                      ccAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCt                                                                                            >  2:206595/1‑140 (MQ=255)                                          aCGGTGCCGGTAAGACCACCACTTTCTACATTGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCca                                                                                        >  1:1267806/1‑140 (MQ=255)                                              tGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAgg                                                                                    >  2:883419/1‑140 (MQ=255)                                                  ggTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGc                                                                                <  1:1106933/140‑1 (MQ=255)                                                    tAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCt                                                                              <  1:883419/140‑1 (MQ=255)                                                      aGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTcc                                                                            >  2:961745/1‑140 (MQ=255)                                                             cacTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTcc                                                                     >  2:401355/1‑140 (MQ=255)                                                             cacTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTcc                                                                     >  2:280544/1‑140 (MQ=255)                                                              acTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCg                                                                    <  1:624959/140‑1 (MQ=255)                                                                    tACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCt                                                              <  1:506649/140‑1 (MQ=255)                                                                         ggTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCAtt                                                                         <  2:223616/124‑1 (MQ=255)                                                                         ggTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCAtt                                                                         >  1:223616/1‑124 (MQ=255)                                                                         ggTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCa                                                            <  2:770212/137‑1 (MQ=255)                                                                         ggTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCa                                                            >  1:770212/1‑137 (MQ=255)                                                                         ggTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCg                                                         >  1:1228678/1‑140 (MQ=255)                                                                          gTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGt                                                        >  2:799467/1‑140 (MQ=255)                                                                             gTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTa                                                     <  1:280544/140‑1 (MQ=255)                                                                              tAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTAc                                                    >  2:1221285/1‑140 (MQ=255)                                                                                 gCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGAt                                                 <  1:206595/140‑1 (MQ=255)                                                                                   aTTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACg                                                   >  1:758417/1‑136 (MQ=255)                                                                                   aTTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACg                                                   <  2:758417/136‑1 (MQ=255)                                                                                     tgtgCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAAcc                                             >  2:985985/1‑140 (MQ=255)                                                                                     tgtgCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAAcc                                             >  1:312684/1‑140 (MQ=255)                                                                                      gtgCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCt                                            <  2:1267806/140‑1 (MQ=255)                                                                                      gtgCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCt                                            <  1:401355/140‑1 (MQ=255)                                                                                       tgCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTg                                           >  2:612526/1‑140 (MQ=255)                                                                                            cgcgATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGc                                      >  2:786992/1‑140 (MQ=255)                                                                                            cgcgATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGc                                      >  2:958333/1‑140 (MQ=255)                                                                                            cgcgATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGc                                      >  1:527992/1‑140 (MQ=255)                                                                                                         aCATCATTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTACCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAAtt                         >  2:1158983/1‑140 (MQ=255)                                                                                                           atcatTGTTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCg                       >  1:843672/1‑140 (MQ=255)                                                                                                           atcatTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCaaa                           <  2:303535/136‑1 (MQ=255)                                                                                                           atcatTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCaaa                           >  1:303535/1‑136 (MQ=255)                                                                                                           atcatTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCg                       >  2:454307/1‑140 (MQ=255)                                                                                                           atcatTATTGATGATGACGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCg                       >  1:271415/1‑140 (MQ=255)                                                                                                                    gatgatgaCGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTgac                   >  1:606435/1‑135 (MQ=255)                                                                                                                    gatgatgaCGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTgac                   <  2:606435/135‑1 (MQ=255)                                                                                                                    gatgatgaCGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACtt              <  1:1036007/140‑1 (MQ=255)                                                                                                                    gatgatgaCGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACtt              >  2:544049/1‑140 (MQ=255)                                                                                                                    gatgatgaCGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACtt              <  1:544049/140‑1 (MQ=255)                                                                                                                    gatgatgaCGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACtt              <  1:209673/140‑1 (MQ=255)                                                                                                                          gaCGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTctgc        >  1:972514/1‑140 (MQ=255)                                                                                                                          gaCGATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTctgc        >  1:917005/1‑140 (MQ=255)                                                                                                                             gATATCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGa     <  1:1023188/140‑1 (MQ=255)                                                                                                                               tatCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac   <  2:527992/140‑1 (MQ=255)                                                                                                                               tatCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac   <  2:1228678/140‑1 (MQ=255)                                                                                                                               tatCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac   <  1:786992/140‑1 (MQ=255)                                                                                                                               tatCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac   >  2:952595/1‑140 (MQ=255)                                                                                                                               tatCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaac   <  2:984520/140‑1 (MQ=255)                                                                                                                                atCAGTCTACTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGaaca  >  1:789023/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    GACCGTCAACTCCGGGGAAATTGTCGGTCTGCTGGGGCCAAACGGTGCCGGTAAGACCACCACTTTCTACATGGTTGTAGGCATTGTGCCGCGCGATGCGGGCAACATCATTATTGATGATGACGATATCAGTCTGCTGCCTCTGCATGCACGCGCGCGCCGCGGTATCGGCTATCTGCCACAGGAAGCCTCCATTTTCCGTCGCCTCAGCGTTTACGATAACCTGATGGCGGTACTGCAAATTCGTGACGACTTGTCTGCTGAACA  >  NC_000913/3344015‑3344281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑