Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,362,541	T→C	F144L (TTC→CTC)	yhcA →	putative fimbrial chaperone YhcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,362,541	0	T	C	100.0%	361.2 / NA	96	F144L (TTC→CTC)	yhcA	putative fimbrial chaperone YhcA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/53);  total (43/53)

AGCTGAAACGCGTAACGTTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAATTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACA  >  NC_000913/3362407‑3362677                                                                                                                                       |                                                                                                                                         agCTGAAACGCGTAACGTTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTg                                                                                                                                     >  1:597936/1‑140 (MQ=255) agCTGAAACGCGTAACGTTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTg                                                                                                                                     >  2:741974/1‑140 (MQ=255)    tGAAACGCGTAACGTTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGaa                                                                                                                                  <  1:1140788/140‑1 (MQ=255)    tGAAACGCGTAACGTTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGaa                                                                                                                                  <  2:292127/140‑1 (MQ=255)    tGAAACGCGTAACGTTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGaa                                                                                                                                  <  2:396317/140‑1 (MQ=255)    tGAAACGCGTAACGTTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGaa                                                                                                                                  <  2:428202/140‑1 (MQ=255)        aCGCGTAACGTTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGc                                                                                                                              >  2:1120534/1‑140 (MQ=255)        aCGCGTAACGTTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGc                                                                                                                              >  1:78294/1‑140 (MQ=255)           cgTAACGTTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTa                                                                                                                           >  2:709996/1‑140 (MQ=255)                       aGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGAcc                                                                                                               <  1:93222/140‑1 (MQ=255)                       aGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGAcc                                                                                                               <  2:78294/140‑1 (MQ=255)                           aTTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAAt                                                                                                           >  2:126430/1‑140 (MQ=255)                           aTTCCACCAAAAGATGACAAGAGCAGCAGAGGTACTGTTTCAATTCGTCAGTATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAAt                                                                                                           >  2:848142/1‑140 (MQ=255)                            ttCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAAtt                                                                                                          >  2:958397/1‑140 (MQ=255)                             tCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTg                                                                                                         >  1:407278/1‑140 (MQ=255)                                 ccaAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAATAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGAt                                                                                                     >  2:152858/1‑140 (MQ=255)                                    aaaGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAg                                                                                                  >  1:576676/1‑140 (MQ=255)                                     aaGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGc                                                                                                 <  1:741974/140‑1 (MQ=255)                                     aaGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGc                                                                                                 <  1:1207009/140‑1 (MQ=255)                                     aaGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGc                                                                                                 <  2:407278/140‑1 (MQ=255)                                     aaGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGc                                                                                                 <  2:777886/140‑1 (MQ=255)                                       gATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCaa                                                                                               <  2:713292/140‑1 (MQ=255)                                        aTGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAAc                                                                                              <  2:373134/140‑1 (MQ=255)                                          gACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAAccc                                                                                            >  1:784665/1‑140 (MQ=255)                                          gACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAAccc                                                                                            <  1:1234716/140‑1 (MQ=255)                                           aCAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAAcccc                                                                                           <  2:576676/140‑1 (MQ=255)                                           aCAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAAcccc                                                                                           <  2:661662/140‑1 (MQ=255)                                            cAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCa                                                                                          >  2:397305/1‑140 (MQ=255)                                             aaGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAg                                                                                         <  1:31041/140‑1 (MQ=255)                                               gagCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTa                                                                                       <  2:358771/140‑1 (MQ=255)                                                agcagcAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAAcccc                                                                                           <  1:1183987/135‑1 (MQ=255)                                                agcagcAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAAcccc                                                                                           >  2:1183987/1‑135 (MQ=255)                                                  cagcagAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAAtt                                                                                    >  2:225783/1‑140 (MQ=255)                                                     cagAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATg                                                                                 <  1:978382/140‑1 (MQ=255)                                                        agTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGtcg                                                                              <  1:225783/140‑1 (MQ=255)                                                          ttACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGtcgtt                                                                            >  2:1169635/1‑140 (MQ=255)                                                          ttACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGtcgtt                                                                            >  1:1137950/1‑140 (MQ=255)                                                          ttACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGtcgtt                                                                            <  2:834445/140‑1 (MQ=255)                                                          ttACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGtcgtt                                                                            >  2:798358/1‑140 (MQ=255)                                                          ttACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGtcgtt                                                                            >  1:269029/1‑140 (MQ=255)                                                          ttACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGtcg                                                                              >  1:549656/1‑138 (MQ=255)                                                          ttACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGtcg                                                                              <  2:549656/138‑1 (MQ=255)                                                                  tCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTa                                                                        <  2:1018702/136‑1 (MQ=255)                                                                  tCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTa                                                                        >  1:1018702/1‑136 (MQ=255)                                                                       tCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTAc                                                               <  2:1137950/140‑1 (MQ=255)                                                                          tCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAAt                                                            >  1:1250230/1‑140 (MQ=255)                                                                          tCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAAt                                                            >  2:1244754/1‑140 (MQ=255)                                                                            aGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAAttt                                                          <  1:1169635/140‑1 (MQ=255)                                                                                tCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTaaaa                                                      >  1:483480/1‑140 (MQ=255)                                                                                  tACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACg                                                    <  2:1250230/140‑1 (MQ=255)                                                                                   aCCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTa                                                                <  1:25502/127‑1 (MQ=255)                                                                                   aCCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTa                                                                >  2:25502/1‑127 (MQ=255)                                                                                   aCCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGc                                                   >  2:575087/1‑140 (MQ=255)                                                                                    ccTGTACTGATCCGCCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCt                                                  <  2:964743/140‑1 (MQ=255)                                                                                    ccTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCt                                                  <  1:1253711/140‑1 (MQ=255)                                                                                        tACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCt                                              >  1:480205/1‑140 (MQ=255)                                                                                        tACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCt                                              >  2:294029/1‑140 (MQ=255)                                                                                         aCTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCtt                                             >  2:800070/1‑140 (MQ=255)                                                                                          cTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTc                                            >  1:466735/1‑140 (MQ=255)                                                                                             aTCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAgg                                         >  2:1261915/1‑140 (MQ=255)                                                                                             aTCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAgg                                         >  2:633139/1‑140 (MQ=255)                                                                                             aTCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAgg                                         >  1:97339/1‑140 (MQ=255)                                                                                             aTCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAgg                                         >  2:520795/1‑140 (MQ=255)                                                                                              tCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGa                                        >  2:877682/1‑140 (MQ=255)                                                                                              tCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGa                                        >  2:1112924/1‑140 (MQ=255)                                                                                               ccACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGaa                                       >  1:251628/1‑140 (MQ=255)                                                                                                  cccGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGaaaaa                                    >  2:851909/1‑140 (MQ=255)                                                                                                  cccGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGaaaaa                                    >  2:654032/1‑140 (MQ=255)                                                                                                   ccGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAAc                                   <  1:643817/140‑1 (MQ=255)                                                                                                     ggCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGTTTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACgg                                 <  1:843081/140‑1 (MQ=255)                                                                                                     ggCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACgg                                 <  1:126430/140‑1 (MQ=255)                                                                                                     ggCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACgg                                 <  1:848142/140‑1 (MQ=255)                                                                                                     ggCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACgg                                 <  2:480205/140‑1 (MQ=255)                                                                                                     ggCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACgg                                 <  2:466735/140‑1 (MQ=255)                                                                                                      gCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACggg                                <  1:958397/140‑1 (MQ=255)                                                                                                      gCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACggg                                <  1:294029/140‑1 (MQ=255)                                                                                                      gCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACggg                                <  1:520795/140‑1 (MQ=255)                                                                                                      gCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACggg                                <  2:252837/140‑1 (MQ=255)                                                                                                       cTTCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACgggg                               >  1:807661/1‑140 (MQ=255)                                                                                                        ttCCCTGCCGGAATAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACggggg                              <  1:152858/140‑1 (MQ=255)                                                                                                        ttCCCTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACggggg                              <  2:483480/140‑1 (MQ=255)                                                                                                          cccTGCCGGAAGAGCGAGAGACCTGGAAACTCCTTGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACgggggtt                            <  2:290646/140‑3 (MQ=255)                                                                                                          cccTGCCGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCt                            <  2:251628/140‑1 (MQ=255)                                                                                                                cGGAAGAGCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTataaat                      <  2:269029/140‑1 (MQ=255)                                                                                                                     gagCGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGAc                 <  1:397305/140‑1 (MQ=255)                                                                                                                        cGAGAGACCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTa              >  1:281093/1‑140 (MQ=255)                                                                                                                        cGAGAGACCTCGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTa              <  1:877682/140‑1 (MQ=255)                                                                                                                         gagagaCCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTAt             >  1:852127/1‑140 (MQ=255)                                                                                                                             gaCCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTc         <  2:852127/140‑1 (MQ=255)                                                                                                                             gaCCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTc         <  1:709996/140‑1 (MQ=255)                                                                                                                             gaCCTGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTc         <  1:798358/140‑1 (MQ=255)                                                                                                                                 tGGAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTAcc     >  1:960641/1‑140 (MQ=255)                                                                                                                                  ggAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACca    <  1:1261915/140‑1 (MQ=255)                                                                                                                                  ggAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACca    <  1:152871/140‑1 (MQ=255)                                                                                                                                  ggAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACca    <  1:633139/140‑1 (MQ=255)                                                                                                                                  ggAAACTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACca    <  1:750616/140‑1 (MQ=255)                                                                                                                                    aaaCTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACcaca  <  1:1251117/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         AGCTGAAACGCGTAACGTTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTTTCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAGACCTGGAAATTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGTAATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCTATAAATAAGACCTATTTTCTACCACA  >  NC_000913/3362407‑3362677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑