BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,414,561	A→G	N233S (AAT→AGT)	acrE →	multidrug efflux pump membrane fusion lipoprotein AcrE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,414,561	0	A	G	100.0%	387.3 / NA	102	N233S (AAT→AGT)	acrE	multidrug efflux pump membrane fusion lipoprotein AcrE
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (55/47); total (55/47)

Only 100 of 103 total aligned reads displayed.

ACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGT  >  NC_000913/3414428‑3414696

aCCGAAGGCGCTCTTGTCACTAATGGGCAAACGATTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCa                                                                                                                                   >  2:424936/1‑140 (MQ=255)

aCCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCa                                                                                                                                   >  2:537213/1‑140 (MQ=255)

    aaGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAg                                                                                                                               >  2:20456/1‑140 (MQ=255)

        cgcTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGaaa                                                                                                                           >  2:727264/1‑140 (MQ=255)

          ctctTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAAc                                                                                                                         >  2:668095/1‑140 (MQ=255)

               gTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGccac                                                                                                                    >  2:1190737/1‑140 (MQ=255)

               gTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGccac                                                                                                                    <  2:780375/140‑1 (MQ=255)

                  aCTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAg                                                                                                                 <  2:40430/140‑1 (MQ=255)

                  aCTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAg                                                                                                                 <  1:643651/140‑1 (MQ=255)

                  aCTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAg                                                                                                                 <  2:557265/140‑1 (MQ=255)

                  aCTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAg                                                                                                                 <  1:989278/140‑1 (MQ=255)

                   cTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGc                                                                                                                <  1:463158/140‑1 (MQ=255)

                       tGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAg                                                                                                                 <  2:441841/135‑1 (MQ=255)

                       tGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAg                                                                                                                 >  1:441841/1‑135 (MQ=255)

                       tGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACg                                                                                                            >  2:1042454/1‑140 (MQ=255)

                       tGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACg                                                                                                            >  1:382316/1‑140 (MQ=255)

                       tGGGCAAACAACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACg                                                                                                            >  2:1164151/1‑140 (MQ=255)

                         ggCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAg                                                                                                                 >  1:900228/1‑133 (MQ=255)

                         ggCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAg                                                                                                                 >  1:935879/1‑133 (MQ=255)

                         ggCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAg                                                                                                                 <  2:935879/133‑1 (MQ=255)

                         ggCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAg                                                                                                                 <  2:900228/133‑1 (MQ=255)

                         ggCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTa                                                                                                          >  1:805287/1‑140 (MQ=255)

                         ggCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTa                                                                                                          >  2:704301/1‑140 (MQ=255)

                                  cTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGATGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTc                                                                                                 >  2:692298/1‑140 (MQ=255)

                                         ggCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGaaa                                                                                          <  1:537213/140‑1 (MQ=255)

                                              cTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGt                                                                                     <  2:821833/140‑1 (MQ=255)

                                                gTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCa                                                                                   <  1:151273/140‑1 (MQ=255)

                                                 tCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCaa                                                                                  >  1:718650/1‑140 (MQ=255)

                                                 tCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCaa                                                                                  >  1:171276/1‑140 (MQ=255)

                                                 tCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCaa                                                                                  >  1:191706/1‑140 (MQ=255)

                                                    agcagcTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAAcc                                                                               <  2:191706/140‑1 (MQ=255)

                                                     gcagcTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCt                                                                              <  1:424936/140‑1 (MQ=255)

                                                       agcTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTAt                                                                            <  1:1042454/140‑1 (MQ=255)

                                                       agcTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTAt                                                                            <  2:1054919/140‑1 (MQ=255)

                                                       agcTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTAt                                                                            <  1:288468/140‑1 (MQ=255)

                                                          tCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCGGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATccc                                                                         <  1:20456/140‑1 (MQ=255)

                                                          tCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATccc                                                                         <  2:718650/140‑1 (MQ=255)

                                                           cGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATcccc                                                                        >  1:126937/1‑140 (MQ=255)

                                                           cGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATcccc                                                                        >  2:848247/1‑140 (MQ=255)

                                                           cGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATcccc                                                                        >  1:251669/1‑140 (MQ=255)

                                                             aTCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCaaa                                                                                 >  1:1081803/1‑129 (MQ=255)

                                                             aTCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCaaa                                                                                 <  2:1081803/129‑1 (MQ=255)

                                                                   tCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCTCTGAAAGGt                                                                >  1:1005160/1‑140 (MQ=255)

                                                                   tCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGt                                                                >  2:1036575/1‑140 (MQ=255)

                                                                    cTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCt                                                                              >  2:615893/1‑125 (MQ=255)

                                                                    cTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCt                                                                              <  1:615893/125‑1 (MQ=255)

                                                                       cGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGc                                                            >  2:424667/1‑140 (MQ=255)

                                                                        gTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCt                                                           >  1:222189/1‑140 (MQ=255)

                                                                        gTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCt                                                           >  1:599631/1‑140 (MQ=255)

                                                                        gTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCt                                                           >  2:267012/1‑140 (MQ=255)

                                                                        gTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCt                                                           >  2:180177/1‑140 (MQ=255)

                                                                        gTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCt                                                           >  2:1253410/1‑140 (MQ=255)

                                                                        gTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCt                                                           >  1:138581/1‑140 (MQ=255)

                                                                        gTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCt                                                           >  1:797564/1‑140 (MQ=255)

                                                                        gTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCt                                                           >  2:643207/1‑140 (MQ=255)

                                                                        gTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCt                                                           >  1:977679/1‑140 (MQ=255)

                                                                        gTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCt                                                           >  1:801366/1‑140 (MQ=255)

                                                                         ttGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCCATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTg                                                          <  2:1183420/140‑1 (MQ=255)

                                                                         ttGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTg                                                          <  2:1247915/140‑1 (MQ=255)

                                                                             tgtgACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGTAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAAt                                                      >  1:988052/1‑140 (MQ=255)

                                                                             tgtgACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCa                                                        <  2:601903/138‑1 (MQ=255)

                                                                             tgtgACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCa                                                        >  1:601903/1‑138 (MQ=255)

                                                                               tgACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACg                                                             <  2:602710/131‑1 (MQ=255)

                                                                               tgACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACg                                                             >  1:602710/1‑131 (MQ=255)

                                                                                 aCCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATtctc                                                  >  2:131740/1‑140 (MQ=255)

                                                                                 aCCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATtctc                                                  >  2:15184/1‑140 (MQ=255)

                                                                                      aTCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGAtg                                             >  2:79922/1‑140 (MQ=255)

                                                                                       tCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGAtgt                                            <  1:273756/140‑1 (MQ=255)

                                                                                         cAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGa                                          <  2:138581/140‑1 (MQ=255)

                                                                                                    ttATGAGGCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGaa                               <  1:1164151/140‑1 (MQ=255)

                                                                                                          ggCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCAcc                         <  2:702588/140‑1 (MQ=255)

                                                                                                          ggCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCAcc                         >  1:1174135/1‑140 (MQ=255)

                                                                                                           gCTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCg                        >  1:851430/1‑140 (MQ=255)

                                                                                                            cTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGTAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCgg                       <  2:988052/140‑1 (MQ=255)

                                                                                                            cTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCgg                       <  2:797564/140‑1 (MQ=255)

                                                                                                            cTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCgg                       <  2:801366/140‑1 (MQ=255)

                                                                                                            cTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCgg                       <  1:692298/140‑1 (MQ=255)

                                                                                                            cTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCgg                       <  1:424667/140‑1 (MQ=255)

                                                                                                            cTGAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCgg                       <  1:1190737/140‑1 (MQ=255)

                                                                                                              gAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCt                     >  2:931706/1‑140 (MQ=255)

                                                                                                              gAAGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCt                     <  1:180177/140‑1 (MQ=255)

                                                                                                               aaGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTc                    >  2:810291/1‑140 (MQ=255)

                                                                                                               aaGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTc                    >  1:1015370/1‑140 (MQ=255)

                                                                                                               aaGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTc                    >  1:838329/1‑140 (MQ=255)

                                                                                                               aaGCAATCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTc                    <  1:79922/140‑1 (MQ=255)

                                                                                                                   aaTCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATa                <  2:599631/140‑1 (MQ=255)

                                                                                                                     tCCGTAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGCCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAAc              >  1:482926/1‑140 (MQ=255)

                                                                                                                         tAGAGCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTa          >  2:840960/1‑140 (MQ=255)

                                                                                                                            agCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGt       <  2:1005160/140‑1 (MQ=255)

                                                                                                                             gCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTg      <  2:977679/140‑1 (MQ=255)

                                                                                                                             gCAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTg      <  1:704301/140‑1 (MQ=255)

                                                                                                                              cAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCgg                       <  1:829084/122‑1 (MQ=255)

                                                                                                                              cAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCgg                       >  2:829084/1‑122 (MQ=255)

                                                                                                                              cAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATACCCCTACGTg      <  2:468110/139‑1 (MQ=255)

                                                                                                                              cAAGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTg      >  1:468110/1‑139 (MQ=255)

                                                                                                                                aGGAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTg   <  1:931706/140‑1 (MQ=255)

                                                                                                                                 ggAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTTTCCGATGTGACCGTTGATGAAAGCACCGGCTCCa                  >  1:213576/1‑124 (MQ=255)

                                                                                                                                 ggAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGt  <  1:840960/140‑1 (MQ=255)

                                                                                                                                 ggAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGt  >  1:483426/1‑140 (MQ=255)

                                                                                                                                 ggAAGTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGt  <  2:171276/140‑1 (MQ=255)

ACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGT  >  NC_000913/3414428‑3414696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`