Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,415,885	C→T	P285S (CCG→TCG)	acrF →	multidrug efflux pump RND permease AcrF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,415,885	0	C	T	100.0%	307.0 / NA	89	P285S (CCG→TCG)	acrF	multidrug efflux pump RND permease AcrF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (47/42);  total (47/42)

GTTTAAAAATCCGGAAGAATTCGGCAAAGTGACCCTGCGCGTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAACCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACAC  >  NC_000913/3415749‑3416018                                                                                                                                         |                                                                                                                                      gTTTAAAAATCCGGAAGAATTCGGCAAAGTGACCCTGCGCGTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATcgg                                                                                                                                    >  1:1138135/1‑140 (MQ=255)    tAAAAATCCGGAAGAATTCGGCAAAGTGACCCTGCGCGTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATcggcgg                                                                                                                                 <  1:858388/140‑1 (MQ=255)    tAAAAATCCGGAAGAATTCGGCAAAGTGACCCTGCGCGTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATcggcgg                                                                                                                                 <  2:673954/140‑1 (MQ=255)          tctggAAGAATTCGGCAAAGTGACCCTGCGCGTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGcc                                                                                                                           <  2:785326/137‑1 (MQ=255)          tCCGGAAGAATTCGGCAAAGTGACCCTGCGCGTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGcc                                                                                                                           <  1:153718/140‑1 (MQ=255)           ccGGAAGAATTCGGCAAAGTGACCCTGCGCGTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCt                                                                                                                          <  2:593253/140‑1 (MQ=255)           ccGGAAGAATTCGGCAAAGTGACCCTGCGCGTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCt                                                                                                                          <  1:164374/140‑1 (MQ=255)            cGGAAGAATTCGGCAAAGTGACCCTGCGCGTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCt                                                                                                                          >  1:1024601/1‑139 (MQ=255)            cGGAAGAATTCGGCAAAGTGACCCTGCGCGTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCt                                                                                                                          <  2:1024601/139‑1 (MQ=255)            cGGAAGAATTCGGCAAAGTGACCCTGCGCGTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTg                                                                                                                         >  1:468985/1‑140 (MQ=255)               aagaaTTCGGCAAAGTGACCCTGCGCGTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTgggg                                                                                                                      <  2:429871/140‑1 (MQ=255)                           aaGTGACCCTGCGCGTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCa                                                                                                          <  1:1272989/140‑1 (MQ=255)                                     gcgcGTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGTATTAAGCTGGCAACCGGCGCGa                                                                                                <  2:1279379/140‑1 (MQ=255)                                     gcgcGTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGa                                                                                                <  2:81041/140‑1 (MQ=255)                                       gcgTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGa                                                                                                >  2:349682/1‑138 (MQ=255)                                       gcgTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGa                                                                                                <  1:349682/138‑1 (MQ=255)                                       gcgTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAAt                                                                                              <  1:721144/140‑1 (MQ=255)                                       gcgTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAAt                                                                                              >  1:439871/1‑140 (MQ=255)                                                gTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTGTCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGAt                                                                                     >  1:879400/1‑140 (MQ=255)                                                gTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGAt                                                                                     >  2:992390/1‑140 (MQ=255)                                                 tGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAAt                                                                                              <  2:235754/130‑1 (MQ=255)                                                 tGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAAt                                                                                              >  1:235754/1‑130 (MQ=255)                                                 tGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATa                                                                                    >  2:1162187/1‑140 (MQ=255)                                                   aCGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATAcc                                                                                  >  2:1223363/1‑140 (MQ=255)                                                   aCGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATAcc                                                                                  >  1:92512/1‑140 (MQ=255)                                                      gCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACcgcg                                                                               >  2:773820/1‑140 (MQ=255)                                                       cTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAAt                                                                                              <  2:1180607/124‑1 (MQ=255)                                                       cTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAAt                                                                                              >  1:1180607/1‑124 (MQ=255)                                                         cGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGctct                                                                                         >  1:926242/1‑127 (MQ=255)                                                         cGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGctct                                                                                         <  2:926242/127‑1 (MQ=255)                                                         cGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGaaa                                                                            >  1:1069091/1‑140 (MQ=255)                                                         cGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGaaa                                                                            <  2:138223/140‑1 (MQ=255)                                                         cGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGaaa                                                                            <  1:596670/140‑1 (MQ=255)                                                          ggtggtACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAg                                                                           <  1:1080225/140‑1 (MQ=255)                                                               tACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCAtt                                                                      <  2:752566/140‑1 (MQ=255)                                                                     tGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCa                                                                >  1:835750/1‑140 (MQ=255)                                                                      gAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCaa                                                               >  2:475479/1‑140 (MQ=255)                                                                      gAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCaa                                                               >  1:510059/1‑140 (MQ=255)                                                                      gAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCaa                                                               >  2:536566/1‑140 (MQ=255)                                                                              tCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCg                                                       >  2:386617/1‑140 (MQ=255)                                                                              tCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCg                                                       >  2:180686/1‑140 (MQ=255)                                                                              tCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCg                                                       <  1:895277/140‑1 (MQ=255)                                                                                gCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGa                                                     >  1:297296/1‑140 (MQ=255)                                                                                 cACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGaa                                                    >  1:313453/1‑140 (MQ=255)                                                                                 cACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGaa                                                    >  2:59689/1‑140 (MQ=255)                                                                                  aCGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAAt                                                   <  2:510059/140‑1 (MQ=255)                                                                                     ggTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTAc                                                >  1:800473/1‑140 (MQ=255)                                                                                        tGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGc                                             >  2:1248374/1‑140 (MQ=255)                                                                                              tGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTAc                                                <  1:767508/131‑1 (MQ=255)                                                                                              tGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTAc                                                >  2:767508/1‑131 (MQ=255)                                                                                              tGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCAttct                                       >  2:4881/1‑140 (MQ=255)                                                                                               ggCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCAttctt                                      >  2:1014439/1‑140 (MQ=255)                                                                                                gCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCCttcttc                                     >  2:1249072/1‑140 (MQ=255)                                                                                                gCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCAttcttc                                     >  1:880774/1‑140 (MQ=255)                                                                                                gCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCAttcttc                                     >  1:702516/1‑140 (MQ=255)                                                                                                gCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCAttcttc                                     >  2:364874/1‑140 (MQ=255)                                                                                                 cGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGGATTACAGCCATTCTTcc                                    >  1:1271755/1‑140 (MQ=255)                                                                                                    tGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGc                                 >  1:26714/1‑140 (MQ=255)                                                                                                    tGAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTCCCCGc                                 >  1:1127476/1‑140 (MQ=255)                                                                                                     gAAAACTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCa                                <  2:835750/140‑1 (MQ=255)                                                                                                     gAAAACAATACAGTTACCGCTCGTATCAACGGAAAATCGCCGGCGGCCGTGGGGATTAAGCCGGCAACCGCCGCGAATGCTCTCGATCCCGCGAAAGCCATTAAGACAAAACTGGCGGAATTACAGCCATTCTTCCCGCa                                <  2:1271755/140‑1 (MQ=255)                                                                                                      aaaaCTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAg                               >  2:791486/1‑140 (MQ=255)                                                                                                      aaaaCTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAg                               >  1:520831/1‑140 (MQ=255)                                                                                                       aaaCTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACa                                               <  2:1109470/123‑1 (MQ=255)                                                                                                       aaaCTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACa                                               >  1:1109470/1‑123 (MQ=255)                                                                                                        aaCTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAggg                             <  2:1241119/140‑1 (MQ=255)                                                                                                          cTATAACGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGaa                           <  1:773820/140‑1 (MQ=255)                                                                                                              aaCGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATTCCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGaa                       <  2:1138135/140‑1 (MQ=255)                                                                                                               aCGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAg                      <  1:475479/140‑1 (MQ=255)                                                                                                               aCGTTATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAg                      <  1:180686/140‑1 (MQ=255)                                                                                                                  ttATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGtt                   >  1:311672/1‑140 (MQ=255)                                                                                                                   tATCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTtc                  >  1:166189/1‑140 (MQ=255)                                                                                                                    aTCGCTCGTGTCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTtct                 <  2:879400/140‑1 (MQ=255)                                                                                                                    aTCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTtct                 >  2:635446/1‑140 (MQ=255)                                                                                                                    aTCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTtct                 <  2:1222301/140‑1 (MQ=255)                                                                                                                     tCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTtctc                >  2:472706/1‑140 (MQ=255)                                                                                                                     tCGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTtctc                >  1:1243119/1‑140 (MQ=255)                                                                                                                      cGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTctct               <  1:472706/140‑1 (MQ=255)                                                                                                                      cGCTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTctct               <  1:4881/140‑1 (MQ=255)                                                                                                                        cTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTAc             <  1:992390/140‑1 (MQ=255)                                                                                                                        cTCGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTAc             <  1:791486/140‑1 (MQ=255)                                                                                                                          cGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTAc             <  2:931121/138‑1 (MQ=255)                                                                                                                          cGTATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTAc             >  1:931121/1‑138 (MQ=255)                                                                                                                            tATCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCtt         >  1:631765/1‑140 (MQ=255)                                                                                                                              tCAACGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTAt       <  1:1162187/140‑1 (MQ=255)                                                                                                                                aaCGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGa     <  1:1223363/140‑1 (MQ=255)                                                                                                                                  cGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGaca   <  1:59689/140‑1 (MQ=255)                                                                                                                                  cGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGaca   <  1:635446/140‑1 (MQ=255)                                                                                                                                  cGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGaca   >  1:429602/1‑140 (MQ=255)                                                                                                                                  cGGAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGaca   >  2:1001645/1‑140 (MQ=255)                                                                                                                                   ggAAAATCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGacac  <  2:26714/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      GTTTAAAAATCCGGAAGAATTCGGCAAAGTGACCCTGCGCGTAAACAGTGACGGCTCGGTGGTACGCCTGAAAGATGTCGCACGGGTTGAACTTGGCGGTGAAAACTATAACGTTATCGCTCGTATCAACGGAAAACCGGCGGCGGGCCTGGGGATTAAGCTGGCAACCGGCGCGAATGCTCTCGATACCGCGAAAGCCATTAAGGCAAAACTGGCGGAATTACAGCCATTCTTCCCGCAGGGAATGAAGGTTCTCTACCCTTATGACAC  >  NC_000913/3415749‑3416018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑